%0 Journal Article %A de Araújo Longo, Luís Guilherme %A Fontana, Herrison %A Santos de Sousa, Viviane %A Chilinque Zambão da Silva, Natalia %A Souto Martins, Ianick %A Meurer Moreira, Beatriz %T Emergence of mgrB locus deletion mediating polymyxin resistance in pandemic KPC-producing Klebsiella pneumoniae ST15 lineage %D 2021 %J Journal of Medical Microbiology, %V 70 %N 3 %@ 1473-5644 %C 001309 %R https://doi.org/10.1099/jmm.0.001309 %K Klebsiella pneumoniae colistin resistance %K mgrB deletion %K multidrug resistance %I Microbiology Society, %X Klebsiella pneumoniae causes a diversity of infections in both healthcare and community settings. This pathogen is showing an increased ability to accumulate antimicrobial resistance and virulence genes, making it a public health concern. Here we describe the whole-genome sequence characteristics of an ST15 colistin-resistant K. pneumoniae isolate obtained from a blood culture of a 79-year-old female patient admitted to a university hospital in Brazil. Kp14U04 was resistant to most clinically useful antimicrobial agents, remaining susceptible only to aminoglycosides and fosfomycin. The colistin resistance in this isolate was due to a ~1.3 kb deletion containing four genes, namely mgrB, yebO, yobH and the transcriptional regulator kdgR. The study isolate presented a variety of antimicrobial resistance genes, including the carbapenemase-encoding gene bla KPC-2, the extended-spectrum beta-lactamase (ESBL)-encoding gene bla SHV-28 and the beta-lactamase-encoding gene bla OXA-1. Additionally, Kp14U04 harboured a multiple stress resistance protein, efflux systems and regulators, heavy metal resistance and virulence genes, plasmids, prophage-related sequences and genomic islands. These features revealed the high potential of this isolate to resist antimicrobial therapy, survive in adverse environments, cause infections and overcome host defence mechanisms. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001309