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Abstract
Klebsiella pneumoniae causes a diversity of infections in both healthcare and community settings. This pathogen is showing an increased ability to accumulate antimicrobial resistance and virulence genes, making it a public health concern. Here we describe the whole-genome sequence characteristics of an ST15 colistin-resistant K. pneumoniae isolate obtained from a blood culture of a 79-year-old female patient admitted to a university hospital in Brazil. Kp14U04 was resistant to most clinically useful antimicrobial agents, remaining susceptible only to aminoglycosides and fosfomycin. The colistin resistance in this isolate was due to a ~1.3 kb deletion containing four genes, namely mgrB, yebO, yobH and the transcriptional regulator kdgR. The study isolate presented a variety of antimicrobial resistance genes, including the carbapenemase-encoding gene bla KPC-2, the extended-spectrum beta-lactamase (ESBL)-encoding gene bla SHV-28 and the beta-lactamase-encoding gene bla OXA-1. Additionally, Kp14U04 harboured a multiple stress resistance protein, efflux systems and regulators, heavy metal resistance and virulence genes, plasmids, prophage-related sequences and genomic islands. These features revealed the high potential of this isolate to resist antimicrobial therapy, survive in adverse environments, cause infections and overcome host defence mechanisms.
- Received:
- Accepted:
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Funding
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Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (BR)
- Principle Award Recipient: BeatrizMeurer Moreira
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Conselho Nacional de Desenvolvimento Científico e Tecnológico
(Award 465718/2014-0)
- Principle Award Recipient: BeatrizMeurer Moreira
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Conselho Nacional de Desenvolvimento Científico e Tecnológico
(Award 88881.068043/2014-01)
- Principle Award Recipient: BeatrizMeurer Moreira
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
(Award 001)
- Principle Award Recipient: LuisGuilherme de Araujo Longo