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Volume 70, Issue 2

Relatedness of type IV pilin PilA amongst geographically diverse isolated from cattle with infectious bovine keratoconjunctivitis Open Access

Abstract

is frequently isolated from the eyes of cattle with infectious bovine keratoconjunctivitis (IBK; pinkeye). As with which has been causally linked to IBK, expresses an RTX (repeats in the structural toxin) cytotoxin that is related to cytotoxin. Pilin, another pathogenic factor in , is required for corneal attachment. Seven antigenically distinct pilin serogroups have been described in .

Multiple different serogroups exist amongst type IV pilin encoded by , however, it is not known whether exhibits a similar degree of diversity in type IV pilin that it encodes.

This study was done to characterize a structural pilin (PilA) encoded by isolated from cases of IBK to determine if diversity exists amongst PilA sequences.

Ninety-four isolates of collected between 2002 and 2017 from 23 counties throughout California and from five counties in four other Western states were evaluated.

DNA sequencing and determination of deduced amino acid sequences revealed ten (designated groups A through J) unique PilA sequences that were ~96.1–99.3 % identical. Pilin groups A and C matched previously reported putative PilA sequences from isolated from IBK-affected cattle in the USA (Virginia, Nebraska, and Kansas) and Asia (Kazakhstan). The ten pilin sequences identified were only ~74–76 % identical to deduced amino acid sequences of putative pilin proteins identified from the previously reported whole-genome sequences of derived from deep nasopharyngeal swabs of IBK-asymptomatic cattle.

Compared to the diversity reported between structural pilin proteins amongst different serogroups of , PilA from geographically diverse isolates derived from IBK-affected cattle are more conserved.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): infectious bovine keratoconjunctivitis , Moraxella bovis , Moraxella bovoculi , PilA , pilin and pinkeye
Funding
This study was supported by the:
  • National Institute of Food and Agriculture (Award 1009855)
    • Principle Award Recipient: JohnA Angelos
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-01-06
2024-04-20
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Relatedness of type IV pilin PilA amongst geographically diverse Moraxella bovoculi isolated from cattle with infectious bovine keratoconjunctivitis
J. Med. Microbiol. 70, 001293 (2021); https://doi.org/10.1099/jmm.0.001293
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