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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 69, Issue 10

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein No Access

Abstract

This study evaluated the effect of etomidate against biofilms of spp. and analysed through molecular docking the interaction of this drug with ALS3, an important protein for fungal adhesion. Three fluconazole-resistant fungi were used: , and . Growing biofilms were exposed to etomidate at 31.25–500 µg ml. Then, an ALS3 adhesive protein from was analysed through a molecular mapping technique, composed of a sequence of algorithms to perform molecular mapping simulation based on classic force field theory. Etomidate showed antifungal activity against growing biofilms of resistant and at all concentrations used in the study. The etomidate coupling analysis revealed three interactions with the residues of interest compared to hepta-threonine, which remained at the ALS3 site. In addition, etomidate decreased the expression of mannoproteins on the surface of . These results revealed that etomidate inhibited the growth of biofilms.

  • Received:
  • Accepted:
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Keyword(s): ALS3 , antifungal activity , biofilm , Candida spp. , etomidate and molecular docking
© 2020 The Authors
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/content/journal/jmm/10.1099/jmm.0.001241
2020-09-07
2026-02-16

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/content/journal/jmm/10.1099/jmm.0.001241
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Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein
J. Med. Microbiol. 69, 1221 (2020); https://doi.org/10.1099/jmm.0.001241
/content/journal/jmm/10.1099/jmm.0.001241
/content/journal/jmm/10.1099/jmm.0.001241
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