1887

Abstract

Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).

Funding
This study was supported by the:
  • Bernard P Fourie , National Research Foundation
  • Tondani Asaph Mboneni , National Research Foundation
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/content/journal/jmm/10.1099/jmm.0.001239
2020-08-05
2020-10-29
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