Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).

This study was supported by the:
  • National Research Foundation
    • Principle Award Recipient: Bernard P Fourie
  • National Research Foundation
    • Principle Award Recipient: Tondani Asaph Mboneni

Article metrics loading...

Loading full text...

Full text loading...



  1. World Health Organization Global Tuberculosis Report 2017 World Health Organization; 2017
    [Google Scholar]
  2. NICD QA Steering Group NICD laboratory Handbook. standard operating procedure. document NIC0104 version 12. http://www.nicd.ac.za/assets/files/NIC104v12.pdf 23 August 2018
  3. Omar SV, Peters RPH, Ismail NA, Dreyer AW, Said HM et al. Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis . J Microbiol Methods 2015; 117:57–63 [View Article][PubMed]
    [Google Scholar]
  4. Daum LT, Peters RPH, Fourie PB, Jonkman K, Worthy SA et al. Molecular detection of Mycobacterium tuberculosis from sputum transported in PrimeStore(®) from rural settings. Int J Tuberc Lung Dis 2015; 19:552–557 [View Article][PubMed]
    [Google Scholar]
  5. Omar SV, Peters RPH, Ismail NA, Jonkman K, Dreyer AW et al. Field evaluation of a novel preservation medium to transport sputum specimens for molecular detection of Mycobacterium tuberculosis in a rural African setting. Trop Med Int Health 2016; 21:776–782 [View Article][PubMed]
    [Google Scholar]
  6. Daum LT, Fourie PB, Peters RPH, Rodriguez JD, Worthy SA et al. Xpert MTB/RIF detection of Mycobacterium tuberculosis from sputum collected in molecular transport medium. Int J Tuberc Lung Dis 2016; 20:1118–1124 [View Article][PubMed]
    [Google Scholar]
  7. Shenai S, Amisano D, Ronacher K, Kriel M, Banada PP et al. Exploring alternative biomaterials for diagnosis of pulmonary tuberculosis in HIV-negative patients by use of the GeneXpert MTB/RIF assay. J Clin Microbiol 2013; 51:4161–4166 [View Article][PubMed]
    [Google Scholar]
  8. Wood RC, Luabeya AK, Weigel KM, Wilbur AK, Jones-Engel L et al. Detection of Mycobacterium tuberculosis DNA on the oral mucosa of tuberculosis patients. Sci Rep 2015; 5:1–5 [View Article][PubMed]
    [Google Scholar]
  9. Feasey NA, Banada PP, Howson W, Sloan DJ, Mdolo A et al. Evaluation of Xpert MTB/RIF for detection of tuberculosis from blood samples of HIV-infected adults confirms Mycobacterium tuberculosis bacteremia as an indicator of poor prognosis. J Clin Microbiol 2013; 51:2311–2316 [View Article][PubMed]
    [Google Scholar]

Data & Media loading...


Supplementary material 1


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error