1887

Abstract

Linezolid-resistant (LZR) has recently emerged in our hospital, and its potential resistance mechanisms are still not clear.

This study aimed to investigate the epidemiology, clinical and genetic characteristics, resistance mechanisms and biofilm formation capacity of LZR s isolated from patients at Huashan Hospital, Shanghai, PR China between 2012 and 2018.

Strains were subjected to antimicrobial susceptibility testing (AST) with antibiotics using the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The presence of , and , as well as mutations in the 23S ribosomal (r)RNA and ribosomal proteins, was investigated using PCR and sequencing techniques. The genetic relationship between isolates was analysed using pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS). Biofilm biomasses were detected by using crystal violet staining.

Twenty-one LZR strains displayed MICs of 32–512 μg ml. All LZR strains showed G2576T and C2104T mutations in the 23S rRNA V region. Besides G2576T and C2104T, no base mutations were detected in the V region. The was detected in 12 strains, while and were not amplified in 21 . strains. PFGE showed that the LZR strains belonged to a single clone. The phylogenetic tree showed that 20 LZR strains were highly similar to LNZR-1, isolated from Harbin (located in the north of China) in 2013, which showed resistance to linezolid.

In this research, -negative strains displayed linezolid MICs of 32 μg ml. In comparison, -positive strains exhibited linezolid MICs of 128–512 μg ml, indicating that high levels of linezolid resistance appear to be related to the presence of . The outbreak of LZR in our hospital needs to be monitored closely.

Funding
This study was supported by the:
  • Dongfang Lin , National Natural Science Foundation of China , (Award 81573470)
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2020-07-30
2020-08-06
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