Evaluation of the usefulness of selected methods for the detection of carbapenemases in strains Free

Abstract

rods, belonging to the family , are generally opportunistic pathogens commonly associated with nosocomial infections, especially in intensive care units. Interestingly, strains of this genus also show multi-drug resistance. In recent years, multiple studies have indicated that the prevalence of carbapenem resistance has increased rapidly among representatives.

The aim of this study was to assess the usefulness of selected phenotypic and genotypic methods for the detection of the most important carbapenemases in strains.

The study involved 51 strains. The ability to produce carbapenemases was determined by phenotypic methods (double disc synergy test, test with four discs and three inhibitors, CarbaNP test, culture on chromogenic medium, panels of automatic method – Phoenix, CIM test and modified Hodge test). The potential for carbapenemase synthesis was also evaluated using real-time PCR, detecting , , and genes.

Using the phenotypic methods, positive results were obtained for all of the analysed strains. Using PCR, carbapenemase synthesis potential was confirmed on the molecular level; the gene was detected in 23 strains, the gene in 26 strains and the gene in two strains.

There was complete agreement between the carbapenemases detected by the genetic method and the results obtained with phenotypic methods.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001202
2020-05-27
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/69/6/792.html?itemId=/content/journal/jmm/10.1099/jmm.0.001202&mimeType=html&fmt=ahah

References

  1. Vanstone GL, Woodhead S, Roulston K, Sharma H, Wey E et al. Improving the detection of carbapenemase-producing organisms (CPO) in a low-prevalence setting: evaluation of four commercial methods and implementation of an algorithm of testing. J Med Microbiol 2018; 67:208–214 [View Article][PubMed]
    [Google Scholar]
  2. Munoz-Price LS, Poirel L, Bonomo RA, Schwaber MJ, Daikos GL et al. Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. Lancet Infect Dis 2013; 13:785–796 [View Article][PubMed]
    [Google Scholar]
  3. Sękowska A, Hryniewicz W, Gniadkowski M, Deptuła A, Kusza K et al. Antimicrobial susceptibility of metallo-beta-lactamase positive and negative Klebsiella pneumoniae strains isolated from intensive care unit patients. Pol J Microbiol 2010; 59:67–69 [View Article][PubMed]
    [Google Scholar]
  4. EUCAST The European Committee on Antimicrobial Susceptibility Testing - EUCAST. https://www.eucast.org ; 2019
  5. Nordmann P, Poirel L, Dortet L. Rapid detection of carbapenemase-producing Enterobacteriaceae . Emerg Infect Dis 2012; 18:1503–1507 [View Article][PubMed]
    [Google Scholar]
  6. van der Zwaluw K, de Haan A, Pluister GN, Bootsma HJ, de Neeling AJ et al. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods. PLoS One 2015; 10:e0123690 [View Article]
    [Google Scholar]
  7. Amjad A, Mirza IA, Abbasi SA, Farwa U, Malik N et al. Modified Hodge test: a simple and effective test for detection of carbapenemase production. Iranian J Microbiol 2011; 3:189–193
    [Google Scholar]
  8. Koppe U, von Laer A, Kroll LE, Noll I, Feig M et al. Carbapenem non-susceptibility of Klebsiella pneumoniae isolates in hospitals from 2011 to 2016, data from the German Antimicrobial Resistance Surveillance (ARS). Antimicrob Res Infect Control 2018; 7:71 [View Article]
    [Google Scholar]
  9. Thomson G, Turner D, Brasso W, Kircher S, Guillet T et al. High-Stringency evaluation of the automated BD Phoenix CPO detect and rapidec Carba NP. Tests for Detection and classification of carbapenemases. J Clin Microbiol 2017; 55:3437–3443 [View Article]
    [Google Scholar]
  10. Baraniak A, Izdebski R, Fiett J, Gawryszewska I, Bojarska K et al. NDM-producing Enterobacteriaceae in Poland, 2012–14: inter-regional outbreak of Klebsiella pneumoniae ST11 and sporadic cases. J Antimicrob Chemother 2016; 71:85–91 [View Article]
    [Google Scholar]
  11. Giske CD, Fröding I, Hasan CM, Turlej-Rogacka A, Toleman M et al. Diverse sequence types of Klebsiella pneumonia . Antimicrob Agents Chemother 2012; 56:2735–2738
    [Google Scholar]
  12. Sonnevend A, Al Baloushi A, Ghazawi A, Hashmey R, Girgis S et al. Emergence and spread United Arab Emirates. J Med Microbiol 2013; 62:1044–1050
    [Google Scholar]
  13. Shoma S, Kamruzzaman M, Ginn AN, Iredell JR, Partridge SR. Characterization of multidrug-resistant Klebsiella pneumoniae from Australia. Diagn Microbiol Infect Dis 2014; 78:93–97 [View Article]
    [Google Scholar]
  14. Jain A, Hopkins KL, Turton J, Doumith M, Hill R et al. Ndm carbapenemsases in United Kingdom. J Ant Chemother 2014; 69:1777–1784
    [Google Scholar]
  15. van Duin D, Doi Y. The global epidemiology of carbapenemase-producing Enterobacteriaceae . Virulence 2017; 8:460–469 [View Article]
    [Google Scholar]
  16. Ojdana D, Sacha PT, Gutowska A, Majewski P, Wieczorek P et al. Antibiotic resistance profiles of KPC and NDM carbapenemases-producing Klebsiella pneumoniae . Zakażenia XXI wieku 2019; 2019:131–137 [View Article]
    [Google Scholar]
  17. Qamar S, Shaheen N, Shakoor S, Farooqi J, Jabeen K et al. Frequency of colistin and fosfomycin resistance in carbepenem-resistant Enterobacteriaceae from a teriary care hospital in Karachi. Infect Drug Resist 2017; 10:231–236 [View Article]
    [Google Scholar]
  18. Falagas ME, Tansarli GS, Karageorgopoulos DE, Vardakas KZ. Deaths attributable to carbapenem-resisatnt Enterobacteriaceae infections. Emerg Infect Dis 2014; 20:1170–1175 [View Article]
    [Google Scholar]
  19. Dortet L, Bréchard L, Poirel L, Nordmann P. Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test. J Med Microbiol 2014; 63:772–776 [View Article]
    [Google Scholar]
  20. Ong CH, Ratnayake L, Ang MLT, Lin RTP, Chan DSG. Diagnostic Accuracy of BD Phoenix CPO Detect for Carbapenemase Production in 190 Enterobacteriaceae Isolates. J Clin Microbiol 2018; 56:Pii: e01043-18. [View Article][PubMed]
    [Google Scholar]
  21. Simon M, Gatermann S, Pfeifer Y, Reischl U, Gessner A et al. Evaluation of the automated BD Phoenix CPO detect panel in combination with the β-CARBA assay for detection and classification of carbapenemase-producing Enterobacterales . J Microbiol Methods 2019; 156:29–33 [View Article]
    [Google Scholar]
  22. Teo JWP, La M-V, Lin RTP. Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD Max open system. Diagn Microbiol Infect Dis 2016; 86:358–361 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001202
Loading
/content/journal/jmm/10.1099/jmm.0.001202
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed