1887

Abstract

An important factor for delayed healing of chronic wounds is the presence of bacteria. Quorum sensing (QS), a cell density-dependent signalling system, controls the production of many virulence factors and biofilm formation in .

Inhibition by sodium salicylate (NaSa) of QS-regulated virulence expression was evaluated in QS-characterized clinical wound isolates of cultured in serum-containing medium.

Fourteen clinical strains from chronic wounds were evaluated for the production of QS signals and virulence factors. Inhibition of QS by NaSa in clinical strains, wild-type PAO1 and QS reporter strains was evaluated using assays for the production of biofilm, pyocyanin, siderophores, alkaline protease, elastase and stapholytic protease.

Six clinical strains secreted several QS-associated virulence factors and signal molecules and two were negative for all factors. Sub-inhibitory concentrations of NaSa downregulated the expression of the QS-related genes , and and reduced the secretion of several virulence factors in PAO1 and clinical strains cultured in serum. Compared to serum-free media, the presence of serum increased the expression of QS genes and production of siderophores and pyocyanin but decreased biofilm formation.

from chronic wound infections showed different virulence properties. While very few strains showed no QS activity, approximately half were highly virulent and produced QS signals, suggesting that the targeting of QS is a viable and relevant strategy for infection control. NaSa showed activity as a QS-inhibitor by lowering the virulence phenotypes and QS signals at both transcriptional and extracellular levels.

Funding
This study was supported by the:
  • Sofia Almqvist , Mölnlycke Health Care AB (SE)
  • Erik Gerner , Mölnlycke Health Care AB (SE)
  • Margarita Trobos , Doctor Felix Neubergh Foundation (SE)
  • Erik Gerner , Doctor Felix Neubergh Foundation (SE)
  • Margarita Trobos , Adlerbertska Stiftelserna
  • Erik Gerner , Adlerbertska Stiftelserna
  • Erik Gerner , Stiftelsen Handlanden Hjalmar Svenssons
  • Margarita Trobos , CARe - Centre for Antibiotic Resistance Research at University of Gothenburg
  • Margarita Trobos , European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 754412 , (Award MoRE2020 - Region Västra Götaland)
  • Margarita Trobos , Stiftelsen för Strategisk Forskning , (Award RMA15-0110 2016)
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2020-04-22
2020-06-02
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