1887

Abstract

The use of silver as an antimicrobial therapeutic is limited by its toxicity to host cells compared with that required to kill bacterial pathogens.

To use aptamer targeting of DNA scaffolded silver nanoclusters as an antimicrobial agent for treating infections.

Antimicrobial activity was assessed in planktonic cultures and using an invertebrate model of infection.

The aptamer conjugates that we call aptabiotics have potent antimicrobial activity. Targeted silver nanoclusters were more effective at killing than the equivalent quantity of untargeted silver nanoclusters. The aptabiotics have an IC of 1.3–2.6 µM against planktonically grown bacteria. Propidium iodide staining showed that they rapidly depolarize bacterial cells to kill approximately 50 % of the population within 10 min following treatment. testing in the model of infection prolonged survival from an otherwise lethal infection.

Using as a model, we show that targeting of DNA-scaffolded silver nanoclusters with an aptamer has effective fast-acting antimicrobial activity and in an animal model.

Funding
This study was supported by the:
  • Darren Day , Wellington Medical Research Foundation , (Award 2015/257)
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2020-03-03
2020-06-03
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