RT Journal Article SR Electronic(1) A1 Hiroi, Satoshi A1 Kawahata, Takuya A1 Furubayashi, KeiichiYR 2020 T1 First isolation of human adenovirus type 85 by molecular analysis of adenoviruses in cases of urethritis JF Journal of Medical Microbiology, VO 69 IS 2 SP 265 OP 269 DO https://doi.org/10.1099/jmm.0.001149 PB Microbiology Society, SN 1473-5644, AB Introduction. Human adenovirus (HAdV) has been reported as a potential cause of sexually transmitted urethritis. Aim. We aimed to investigate HAdVs associated with urethritis in Osaka, Japan through molecular characterization. Methodology. Urine samples were obtained from male patients with urethritis from 2015 to 2018. Molecular analysis of the isolated strains and follow-up real-time polymerase chain reaction analyses of the clinical samples were performed. Results. The isolates were classified into five types belonging to species D (18 cases) or E (one case). HAdV-85 (species D) was detected for the first time in a urethritis case. Follow-up examination demonstrated that HAdV was isolated from urine samples half a month after the first sampling in four cases, and that viral DNA could be detected after 1 month in two cases. Conclusion. The HAdV types detected from urethritis cases were related to respiratory and ocular HAdV infections, while a novel HAdV type identified as a cause of conjunctivitis also causes urethritis. Sexual contact should be avoided for 1 month after HAdV genital infection., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001149