1887

Abstract

Autoinducer-2 (AI-2) quorum sensing is a bacterial communication system that responds to cell density. The system requires activity to produce AI-2, which can regulate gene expression and processes such as biofilm formation.

To investigate the role of in biofilm formation and gene expression in the nosocomial pathogen .

A gene deletion was made in KP563, an extensively drug-resistant isolate. AI-2 production was assessed in wild-type and strains grown in media supplemented with different carbohydrates. Potential roles of in biofilm formation were investigated using a microtiter plate biofilm assay and scanning electron microscopy. Quantitative RT-PCR evaluated the expression of lipopolysaccharide ( and ), polysaccharide (), and type 3 fimbriae () synthesis genes in wild-type and mutant biofilm extracts.

AI-2 production was dependent on the presence of . AI-2 accumulation was highest during early stationary phase in media supplemented with glucose, sucrose or glycerol. Changes in biofilm architecture were observed in the mutant, with less surface coverage and reduced macrocolony formation; however, no differences in biofilm formation between the wild-type and mutant using a microtiter plate assay were observed. In mutant biofilm extracts, the expression of was down-regulated, and the expression of , which encodes a porin for poly-β−1,6-N-acetyl--glucosamine (PNAG) polysaccharide secretion, was upregulated.

Relationships among AI-2-mediated quorum sensing, biofilm formation and gene expression of outer-membrane components were identified in . These inter-connected processes could be important for bacterial group behaviour and persistence.

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/content/journal/jmm/10.1099/jmm.0.001148
2020-01-28
2020-02-28
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