1887

Abstract

Transmission of Enterobacterales in neonatal intensive care units (NICU) can cause outbreaks of colonization and invasive infections among neonates. Two clusters of nosocomial transmission of identified by MALDI-ToF mass-spectrometry were suspected at two NICUs in July and August 2016.

To assess the potential transmission of among neonates.

Whole-genome sequencing (WGS) was performed of isolates obtained through targeted surveillance of patients and environmental sampling.

WGS data revealed that patient and environmental isolates represented two species, and . Core-genome multi-locus sequence typing (cgMLST) of the isolates identified three separate transmission clusters, in Hospital A a cluster of isolates in 12 children and two environmental samples and a second cluster of isolates in five children. In Hospital B a cluster of isolates from three children and five unrelated isolates of and two unrelated isolates of were found.

can cause hospital outbreaks of colonization and infection similar to other spp.

Preliminary results from this study were presented at the 27th European Congress of Clinical Microbiology and Infectious Diseases, April 22-25, 2018, Vienna, Austria.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001143
2020-03-03
2020-06-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/69/3/396.html?itemId=/content/journal/jmm/10.1099/jmm.0.001143&mimeType=html&fmt=ahah

References

  1. Brisse S, Grimont F, Grimont PAD. The genus Klebsiella . In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. (editors) The Prokaryotes: Volume 6: Proteobacteria: Gamma Subclass New York, NY: Springer New York; 2006 pp 159–196
    [Google Scholar]
  2. Rosenblueth M, Martínez L, Silva J, Martínez-Romero E. Klebsiella variicola, a novel species with clinical and plant-associated isolates. Syst Appl Microbiol 2004; 27:27–35 [CrossRef]
    [Google Scholar]
  3. Maatallah M, Vading M, Kabir MH, Bakhrouf A, Kalin M et al. Klebsiella variicola is a frequent cause of bloodstream infection in the Stockholm area, and associated with higher mortality compared to K. pneumoniae . PLoS One 2014; 9:e113539 [CrossRef]
    [Google Scholar]
  4. Seki M, Gotoh K, Nakamura S, Akeda Y, Yoshii T et al. Fatal sepsis caused by an unusual Klebsiella species that was misidentified by an automated identification system. J Med Microbiol 2013; 62:801–803 [CrossRef]
    [Google Scholar]
  5. Long SW, Linson SE, Ojeda Saavedra M, Cantu C, Davis JJ et al. Whole-genome sequencing of human clinical Klebsiella pneumoniae isolates reveals misidentification and misunderstandings of Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae . mSphere 2017; 2: [CrossRef]
    [Google Scholar]
  6. Holt KE, Wertheim H, Zadoks RN, Baker S, Whitehouse CA et al. Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc Natl Acad Sci U S A 2015; 112:E3574–E3581 [CrossRef]
    [Google Scholar]
  7. Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD et al. Extended-Spectrum beta-lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV- and CTX-M-type beta-lactamases. Antimicrob Agents Chemother 2003; 47:3554–3560 [CrossRef]
    [Google Scholar]
  8. Haller S, Eller C, Hermes J, Kaase M, Steglich M et al. What caused the outbreak of ESBL-producing Klebsiella pneumoniae in a neonatal intensive care unit, Germany 2009 to 2012? Reconstructing transmission with epidemiological analysis and whole-genome sequencing. BMJ Open 2015; 5:e007397 [CrossRef]
    [Google Scholar]
  9. Parm Ü., Metsvaht T, Sepp E, Ilmoja M-L, Pisarev H et al. Mucosal surveillance cultures in predicting gram-negative late-onset sepsis in neonatal intensive care units. J Hosp Infect 2011; 78:327–332
    [Google Scholar]
  10. Di DYW, Jang J, Unno T, Hur H-G. Emergence of Klebsiella variicola positive for NDM-9, a variant of new Delhi metallo-β-lactamase, in an urban river in South Korea. J Antimicrob Chemother 2017; 72:1063–1067 [CrossRef]
    [Google Scholar]
  11. Lu Y, Feng Y, McNally A, Zong Z. Occurrence of colistin-resistant hypervirulent Klebsiella variicola . J Antimicrob Chemother 2018; 73:3001–3004 [CrossRef]
    [Google Scholar]
  12. Farzana R, Jones LS, Rahman MA, Andrey D, Sands K et al. Outbreak of hypervirulent multi-drug resistant Klebsiella variicola causing high mortality in neonates in Bangladesh. Clin Infect Dis 2019; 68:1225–1227 [CrossRef]
    [Google Scholar]
  13. Jünemann S, Sedlazeck FJ, Prior K, Albersmeier A, John U et al. Updating benchtop sequencing performance comparison. Nat Biotechnol 2013; 31:294–296 [CrossRef]
    [Google Scholar]
  14. Flandrois J-P, Perrière G, Gouy M. leBIBIQBPP: a set of databases and a webtool for automatic phylogenetic analysis of prokaryotic sequences. BMC Bioinformatics 2015; 16:251 [CrossRef]
    [Google Scholar]
  15. Fonseca EL, Ramos ND, Andrade BGN, Morais LLCS, Marin MFA et al. A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diagn Microbiol Infect Dis 2017; 87:315–317 [CrossRef]
    [Google Scholar]
  16. Imai K, Ishibashi N, Kodana M, Tarumoto N, Sakai J et al. Clinical characteristics in blood stream infections caused by Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae: a comparative study, Japan, 2014-2017. BMC Infect Dis 2019; 19:946
    [Google Scholar]
  17. Potter RF, Lainhart W, Twentyman J, Wallace MA, Wang B et al. Population structure, antibiotic resistance, and uropathogenicity of Klebsiella variicola. mBio 2018; 9:e02481–18
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001143
Loading
/content/journal/jmm/10.1099/jmm.0.001143
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error