%0 Journal Article %A García-Reyes, Selene %A Soberón-Chávez, Gloria %A Cocotl-Yanez, Miguel %T The third quorum-sensing system of Pseudomonas aeruginosa: Pseudomonas quinolone signal and the enigmatic PqsE protein %D 2020 %J Journal of Medical Microbiology, %V 69 %N 1 %P 25-34 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.001116 %K quorum-sensing %K pyocyanin %K Pseudomonas aeruginosa %K 2-alkyl-4-quinolone %K virulence %I Microbiology Society, %X Pseudomonas aeruginosa is an opportunistic pathogen that produces several virulence factors such as lectin A, pyocyanin, elastase and rhamnolipids. These compounds are controlled transcriptionally by three quorum-sensing circuits, two based on the synthesis and detection of N-acyl-homoserine-lactone termed the Las and Rhl system and a third system named the Pseudomonas quinolone signal (PQS) system, which is responsible for generating 2-alkyl-4(1 h)-quinolones (AQs). The transcriptional regulator called PqsR binds to the promoter of pqsABCDE in the presence of PQS or HHQ creating a positive feedback-loop. PqsE, encoded in the operon for AQ synthesis, is a crucial protein for pyocyanin production, activating the Rhl system by a still not fully understood mechanism. In turn, the regulation of the PQS system is modulated by Las and Rhl systems, which act positively and negatively, respectively. This review focuses on the PQS system, from its discovery to its role in Pseudomonas pathogenesis, such as iron depletion and pyocyanin synthesis that involves the PqsE protein – an intriguing player of this system. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001116