Microcolonies: a novel morphological form of pathogenic spp. Free

Abstract

The class unites cell wall lacking bacteria many of which are membrane parasites and opportunistic bacteria.

. This study describes a novel morphological form found in the five species belonging to the bacterial class Mollicutes, and referred to as microcolonies (MCs).

MCs were obtained as described below and characterized with bacteriological and immunological methods, and microscopy.

In contrast to typical colonies (TCs), MCs are characterized by tiny propeller-shaped colonies formed by rod-like cells tightly packed in parallel rows. These colonies were observed within routinely cultivated cultures of type strains 7–12 days post-plating. Rod-like cells were visualized using a scanning electron microscope within TCs with a ‘fried-egg-like’ appearance. MCs were not observed to revert to TCs. MCs were resistant to antibiotics and other treatments effective against TCs. Pure MC cultures were generated by treatment of cultures with hyperimmune serum, antibiotics or argon non-thermal plasma. MCs of strain H-34 were characterized in detail to confirm that they belonged to that species. MCs tested positive via PCR with -specific primers, direct fluorescence and epifluorescence tests, and Western blotting with the camel-derived nanobody aMh-FcG2a, which is specific to the MH3620 transporter protein. Meanwhile, MCs behaved differently in standard bacteriological tests. Pure MC cultures were also isolated directly from clinical samples of the serum, synovial liquid and urine of patients within flammatory urogenital tract diseases, asthma or arthritis. In total, 79 independent MC cultures were isolated from clinical samples including (=70), (=2), (=2) and spp. (=5).

. MCs play an unknown role in infection pathology and display prominent antibiotic resistance, making them a challenge for the future studies on Mollicutes.

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2019-10-31
2024-03-28
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References

  1. Razin S, Hayflick L. Highlights of mycoplasma research--an historical perspective. Biologicals 2010; 38:183–190 [View Article]
    [Google Scholar]
  2. Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas . Microbiol Mol Biol Rev 1998; 62:1094–1156
    [Google Scholar]
  3. Hayflick L, Chanock RM. Mycoplasma species of man. Bacteriol Rev 1965; 29:185–221
    [Google Scholar]
  4. Narita M. Pathogenesis of extrapulmonary manifestations of Mycoplasma pneumoniae infection with special reference to pneumonia. J Infect Chemother 2010; 16:162–169 [View Article]
    [Google Scholar]
  5. Daley GM, Russell DB, Tabrizi SN, McBride J. Mycoplasma genitalium: a review. Int J STD AIDS 2014; 25:475–487 [View Article]
    [Google Scholar]
  6. Othman N, Isaacs D, Daley AJ, Kesson AM. Mycoplasma pneumoniae infection in a clinical setting. Pediatr Int 2008; 50:662–666 [View Article]
    [Google Scholar]
  7. Murtha AP, Edwards JM. The role of mycoplasma and ureaplasma in adverse pregnancy outcomes. Obstet Gynecol Clin North Am 2014; 41:615–627 [View Article]
    [Google Scholar]
  8. Rakovskaia I V, Gorina LG, Balabanov DN, Levina GA, Barkhatova OI et al. [Generalized mycoplasma infection in patients and carriers]. Zh Mikrobiol Epidemiol Immunobiol 2013; 2:37–43
    [Google Scholar]
  9. Watanabe H, Uruma T, Nakamura H, Aoshiba K. The role of Mycoplasma pneumoniae infection in the initial onset and exacerbations of asthma. Allergy Asthma Proc 2014; 35:204–210 [View Article]
    [Google Scholar]
  10. Yano T, Ichikawa Y, Komatu S, Arai S, Oizumi K. Association of Mycoplasma pneumoniae antigen with initial onset of bronchial asthma. Am J Respir Crit Care Med 1994; 149:1348–1353 [View Article]
    [Google Scholar]
  11. Waites KB, Xiao L, Liu Y, Balish MF, Atkinson TP. Mycoplasma pneumoniae from the respiratory tract and beyond. Clin Microbiol Rev 2017; 30:747–809 [View Article]
    [Google Scholar]
  12. Bredt W. Celular morphology of newly isolated Mycoplasma hominis strains. J Bacteriol 1971; 105:449–450
    [Google Scholar]
  13. Razin S. Methods of Mycoplasma . In Razin S, Tully J. eds New York: Academic Press; 1983 pp 83–86
  14. Klieneberger-Nobel E. Mycoplasma, a brief historical review. Ann N Y Acad Sci 1967; 143:713–718 [View Article]
    [Google Scholar]
  15. Dybvig K, Simecka JW, Watson HL, Cassell GH. High-frequency variation in Mycoplasma pulmonis colony size. J Bacteriol 1989; 171:5165–5168 [View Article]
    [Google Scholar]
  16. Kammer GM, Pollack JD, Klainer AS. Scanning-beam electron microscopy of Mycoplasma pneumoniae . J Bacteriol 1971; 104:499–502
    [Google Scholar]
  17. Boatman E. Morphology and ultrastucture of mycoplasmatales. In Barrile M, Razin S. eds The Mycoplasmas. V. 1, Cell Biology New York: Academic Press; 1979 pp 63–102
    [Google Scholar]
  18. Quinlan DC, Maniloff J. Deoxyribonucleic acid synthesis in synchronously growing Mycoplasma gallisepticum. J Bacteriol 1973; 115:117–120
    [Google Scholar]
  19. Burmistrova DA, Tillib SV, Shcheblyakov DV, Dolzhikova IV, Shcherbinin DN et al. Genetic passive immunization with adenoviral vector expressing chimeric nanobody-Fc molecules as therapy for genital infection caused by Mycoplasma hominis. PLoS One 2016; 11:e0150958 [View Article]
    [Google Scholar]
  20. Razin S, Rottem S. Techniques for the manipulation of Mycoplasma membranes. In Madd A. edr Biochemical Analysis of Membranes London: Academic Press; 1976
    [Google Scholar]
  21. Senterfit L. Preparation of antigens and antisera. Methods in Mycoplasmology, V. 2 New York: Academic Press; 1983 pp 401–404
    [Google Scholar]
  22. Del Guidice RA, Barile MF. Immunofluorescent procedures for Mycoplasma identification. Dev Biol Stand 1974; 23:134–137
    [Google Scholar]
  23. Bradbury JM, Oriel CA, Jordan FT. Simple method for immunofluorescent identification of Mycoplasma colonies. J Clin Microbiol 1976; 3:449–452
    [Google Scholar]
  24. Shimizu T, Steffes B, Pompl R, Jamitzky F, Bunk W et al. Characterization of microwave plasma torch for decontamination. Plasma Process Polym 2008; 5:577–582 [View Article]
    [Google Scholar]
  25. Ermolaeva SA, Rakovskaya IV, Miller GG, Sysolyatina EV, Mukhachev AY et al. Nonthermal plasma affects viability and morphology of Mycoplasma hominis and Acholeplasma laidlawii . J Appl Microbiol 2014; 116:1129–1136 [View Article]
    [Google Scholar]
  26. Tourtellotte ME, Jacobs RE. Physiological and serologic comparisons of PPLO from various sources. Ann N Y Acad Sci 1960; 79:521–530 [View Article]
    [Google Scholar]
  27. Hahn RG, Kenny GE. Differences in arginine requirement for growth among arginine-utilizing Mycoplasma species. J Bacteriol 1974; 117:611–618
    [Google Scholar]
  28. Hayflick L. The Mycoplasmatales and the L-Phase of Bacteria Mycoplasma New York: Academic Press; 1969
    [Google Scholar]
  29. Levina G, Barkhatova O, Rakovaskaya I. Stress causes formation of morphologically new type of Mycoplasma hominis. FEBS OPEN Bio 2018144
    [Google Scholar]
  30. Maniloff; J, Nealson K, Psenner R, Maria L, Robert F. Nannobacteria: size limits and evidence. Science 1997; 276:1776 [View Article]
    [Google Scholar]
  31. Kajander EO, Ciftçioglu N, Olavi Kajander E, Seegmiller JE. Nanobacteria: an alternative mechanism for pathogenic intra- and extracellular calcification and stone formation. Proc Natl Acad Sci USA 1998; 95:8274–8279 [View Article]
    [Google Scholar]
  32. Keren I, Kaldalu N, Spoering A, Wang Y, Lewis K. Persister cells and tolerance to antimicrobials. FEMS Microbiol Lett 2004; 230:13–18 [View Article]
    [Google Scholar]
  33. Kussell E, Leibler S. Phenotypic diversity, population growth, and information in fluctuating environments. Science 2005; 309:2075–2078 [View Article]
    [Google Scholar]
  34. Lewis K. Persister cells. Annu Rev Microbiol 2010; 64:357–372 [View Article]
    [Google Scholar]
  35. Proctor RA, Kriegeskorte A, Kahl BC, Becker K, Löffler B et al. Staphylococcus aureus small colony variants (SCVs): a road map for the metabolic pathways involved in persistent infections. Front Cell Infect Microbiol 2014; 4:99 [View Article]
    [Google Scholar]
  36. Edwards AM. Phenotype switching is a natural consequence of Staphylococcus aureus replication. J Bacteriol 2012; 194:5404–5412 [View Article]
    [Google Scholar]
  37. Kriegeskorte A, Lorè NI, Bragonzi A, Riva C, Kelkenberg M et al. Thymidine-dependent staphylococcus aureus small-colony variants are induced by trimethoprim-sulfamethoxazole (SXT) and have increased fitness during SXT challenge. Antimicrob Agents Chemother 2015; 59:7265–7272 [View Article]
    [Google Scholar]
  38. Painter KL, Strange E, Parkhill J, Bamford KB, Armstrong-James D et al. Staphylococcus aureus adapts to oxidative stress by producing H2O2-resistant small-colony variants via the SOS response. Infect Immun 2015; 83:1830–1844 [View Article]
    [Google Scholar]
  39. Proctor RA, van Langevelde P, Kristjansson M, Maslow JN, Arbeit RD. Persistent and relapsing infections associated with small-colony variants of Staphylococcus aureus. Clin Infect Dis 1995; 20:95–102 [View Article]
    [Google Scholar]
  40. Lysnyansky I, Ayling RD. Mycoplasma bovis: mechanisms of resistance and trends in antimicrobial susceptibility. Front Microbiol 2016; 7:595 [View Article]
    [Google Scholar]
  41. Ito S, Shimada Y, Yamaguchi Y, Yasuda M, Yokoi S et al. Selection of Mycoplasma genitalium strains harbouring macrolide resistance-associated 23S rRNA mutations by treatment with a single 1 G dose of azithromycin. Sex Transm Infect 2011; 87:412–414 [View Article]
    [Google Scholar]
  42. Dégrange S, Renaudin H, Charron A, Pereyre S, Bébéar C et al. Reduced susceptibility to tetracyclines is associated in vitro with the presence of 16S rRNA mutations in Mycoplasma hominis and Mycoplasma pneumoniae . J Antimicrob Chemother 2008; 61:1390–1392 [View Article]
    [Google Scholar]
  43. Amram E, Mikula I, Schnee C, Ayling RD, Nicholas RAJ et al. 16S rRNA gene mutations associated with decreased susceptibility to tetracycline in Mycoplasma bovis . Antimicrob Agents Chemother 2015; 59:796–802 [View Article]
    [Google Scholar]
  44. Balwit JM, van Langevelde P, Vann JM, Proctor RA. Gentamicin-Resistant menadione and hemin auxotrophic Staphylococcus aureus persist within cultured endothelial cells. J Infect Dis 1994; 170:1033–1037 [View Article]
    [Google Scholar]
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