@article{mbs:/content/journal/jmm/10.1099/jmm.0.001076, author = "Cardenas-Alvarez, Maria X. and Townsend Ramsett, Megan K. and Malekmohammadi, Sahar and Bergholz, Teresa M.", title = "Evidence of hypervirulence in Listeria monocytogenes clonal complex 14", journal= "Journal of Medical Microbiology", year = "2019", volume = "68", number = "11", pages = "1677-1685", doi = "https://doi.org/10.1099/jmm.0.001076", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001076", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "Galleria mellonella", keywords = "virulence", keywords = "clonal complex", keywords = "Listeria monocytogenes", keywords = "clinical outcomes", abstract = " Purpose. Listeria monocytogenes is a foodborne pathogen that causes central nervous system (CNS) and maternal-neonatal (MN) infections, bacteremia (BAC), and gastroenteritis in humans and ruminants. Specific clonal complexes (CC) have been associated with severe listeriosis cases, however, less is known about differences among subgroup virulence patterns. This study aimed to assess variation in virulence across different CC and clinical outcomes. Methodology. Galleria mellonella larvae were used to compare virulence phenotypes of 34 L . monocytogenes strains representing isolates from CC1, CC6 (from lineage I), and CC7, CC9, CC14, CC37 and CC204 (from lineage II) classified by clinical outcome: BAC, CNS and MN infection. Larvae survival, LD50, cytotoxicity, health index scores and bacterial concentrations post-infection were evaluated as quantifiable indicators of virulence. Results. Isolates belonging to CC14 and MN-associated infections are hypervirulent in G. mellonella as they led to lower G. mellonella survival rates and health index scores, as well as reduced cytotoxic effects when compared to other CC and clinical outcomes included here. CC14 isolates also showed increased bacterial concentrations at 8 and 24 h post-infection, indicating ability to survive the initial immune response and proliferate within G. mellonella larvae. Conclusion. Subgroups of L. monocytogenes possess different virulence phenotypes that may be associated with niche-specificity. While hypervirulent clones have been identified so far in lineage I, our data demonstrate that hypervirulent clones are not restricted to lineage I, as CC14 belongs to lineage II. Identification of subgroups with a higher ability to cause disease may facilitate surveillance and management of listeriosis.", }