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Volume 69, Issue 2

Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults Open Access

Abstract

. The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.

. Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.

. In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.

. Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): influenza , lineage , multiplex PCR , subtype and validation
Funding
This study was supported by the:
  • Influenza virus surveillance was funded by the Public Health Agency of Canada (PHAC), the Canadian Institutes of Health Research (CIHR), and through a Collaborative Research Agreement between GlaxoSmithKline Biologicals SA, and the CIRN SOS Network (Award PCIRN 96974 and 124309, and CIRN 137470)
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2019-07-02
2024-04-19
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Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults
J. Med. Microbiol. 69, 256 (2020); https://doi.org/10.1099/jmm.0.001032
/content/journal/jmm/10.1099/jmm.0.001032
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