1887

Abstract

The aim of the present study was to report the molecular characterization of human group A rotaviruses (RVAs) circulating in Tunisia. Stool specimens were collected from children under 5 years of age who had been hospitalized or were consulting for gastroenteritis in Tunisian hospitals between 2015 and 2017. All samples were screened by reverse-transcription polymerase chain reaction (RT-PCR) for the detection of the VP6 gene specific for RVA. RVA-positive samples were further analysed for G/P genotyping by semi-nested multiplex RT-PCR. Among 454 tested samples, 72 (15.8 %) were positive for RVA. G1P[8] was the most prevalent detected strain (41.7%), followed by G9P[8] (32.8%), G2P[4] (7.5%), G12P[8] (7.5%), G1P[6] (3.0%), G2P[8] (1.5%) and G3P[8] (1.5%), with mixed infections in 4.5 % of cases. In the absence of a national anti-rotavirus vaccination strategy, RVAs remain the primary aetiological agent for gastroenteritis in Tunisian children.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001031
2019-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/68/8/1240.html?itemId=/content/journal/jmm/10.1099/jmm.0.001031&mimeType=html&fmt=ahah

References

  1. Tate JE, Burton AH, Boschi-Pinto C, Parashar UD. Global, regional, and national estimates of rotavirus mortality in children <5 years of age, 2000–2013. Clin Infect Dis 2016; 62:S96–S105 [View Article]
    [Google Scholar]
  2. WHO Rotavirus vaccines. Wkly Epidemiol Rec 2009; 84:533–540
    [Google Scholar]
  3. Dennehy PH. Rotavirus vaccines: an overview. Clin Microbiol Rev 2008; 21:198–208 [View Article]
    [Google Scholar]
  4. Matthijnssens J, Otto PH, Ciarlet M, Desselberger U, Van Ranst M et al. VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol 2012; 157:1177–1182 [View Article]
    [Google Scholar]
  5. Mihalov-Kovács E, Gellért Á, Marton S, Farkas SL, Fehér E et al. Candidate new rotavirus species in sheltered dogs, Hungary. Emerg Infect Dis 2015; 21:660–663 [View Article]
    [Google Scholar]
  6. Bányai K, Kemenesi G, Budinski I, Földes F, Zana B et al. Candidate new rotavirus species in Schreiber's bats, Serbia. Infect Genet Evol 2017; 48:19–26 [View Article]
    [Google Scholar]
  7. Estes MK, Greenberg HB. Rotaviruses. In Fields Virology, 6 ed. Philadelphia, PA: Lippincott Williams and Wilkins; 2013
    [Google Scholar]
  8. Rojas MA, Gonçalves JLS, Dias HG, Manchego A, Santos N. Identification of two novel Rotavirus A genotypes, G35 and P[50], from Peruvian alpaca faeces. Infect Genet Evol 2017; 55:71–74 [View Article]
    [Google Scholar]
  9. Santos N, Hoshino Y. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 2005; 15:29–56 [View Article]
    [Google Scholar]
  10. Chouikha A, Fredj MB, Fodha I, Mathlouthi I, Ardhaoui M et al. [Evolution of group A rotavirus strains circulating in Tunisia over a 3-year period (2005–2007)]. Pathol Biol 2011; 59:e79–e83 [View Article]
    [Google Scholar]
  11. Iturriza Gómara M, Wong C, Blome S, Desselberger U, Gray J. Molecular characterization of VP6 genes of human rotavirus isolates: correlation of genogroups with subgroups and evidence of independent segregation. J Virol 2002; 76:6596–6601 [View Article]
    [Google Scholar]
  12. Trabelsi A, Peenze I, Pager C, Jeddi M, Steele D. Distribution of rotavirus VP7 serotypes and VP4 genotypes circulating in Sousse, Tunisia, from 1995 to 1999: emergence of natural human reassortants. J Clin Microbiol 2000; 38:3415–3419
    [Google Scholar]
  13. Sdiri-Loulizi K, Gharbi-Khélifi H, de Rougemont A, Chouchane S, Sakly N et al. Acute infantile gastroenteritis associated with human enteric viruses in Tunisia. J Clin Microbiol 2008; 46:1349–1355 [View Article]
    [Google Scholar]
  14. Soltani MS, Salah AB, Bouanene I, Trabelsi A, Sfar MT et al. Epidemiology and medical cost of hospitalization due to rotavirus gastroenteritis among children under 5 years of age in the central-east of Tunisia. East Mediterr Health J 2015; 21:584–590 [View Article]
    [Google Scholar]
  15. Hassine-Zaafrane M, Sdiri-Loulizi K, Ben Salem I, Kaplon J, Ayouni S et al. The molecular epidemiology of circulating rotaviruses: three-year surveillance in the region of Monastir, Tunisia. BMC Infect Dis 2011; 11:266 [View Article]
    [Google Scholar]
  16. Moussa A, Ben Hadj Fredj M, Fodha I, BenHamida-Rebaï M, Kacem S et al. Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: emergence of the genotype G12. J Med Microbiol 2016; 65:1028–1037 [View Article]
    [Google Scholar]
  17. Chouikha A, Fodha I, Bouslama L, Ben Hadj Fredj M, Jaoua S et al. Emergence and characterization of human rotavirus G9 strains in Tunisia. J Infect Dis 2009; 200:S239–S243 [View Article]
    [Google Scholar]
  18. Mwenda JM, Burke RM, Shaba K, Mihigo R, Tevi-Benissan MC et al. Progress with the implementation of rotavirus surveillance and vaccines in countries of the WHO African region, 2007–2016. Wkly Epidemiol Rec 2017; 92:673–680
    [Google Scholar]
  19. Cook SM, Glass RI, LeBaron CW, Ho MS. Global seasonality of rotavirus infections. Bull World Health Organ 1990; 68:171–177
    [Google Scholar]
  20. Simmonds MK, Armah G, Asmah R, Banerjee I, Damanka S et al. New oligonucleotide primers for P-typing of rotavirus strains: strategies for typing previously untypeable strains. J Clin Virol 2008; 42:368–373 [View Article]
    [Google Scholar]
  21. Das BK, Gentsch JR, Cicirello HG, Woods PA, Gupta A et al. Characterization of rotavirus strains from newborns in New Delhi, India. J Clin Microbiol 1994; 32:1820–1822
    [Google Scholar]
  22. Iturriza-Gómara M, Green J, Brown DW, Desselberger U, Gray JJ. Diversity within the VP4 gene of rotavirus P[8] strains: implications for reverse transcription-PCR genotyping. J Clin Microbiol 2000; 38:898–901
    [Google Scholar]
  23. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M et al. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 1992; 30:1365–1373
    [Google Scholar]
  24. Samajdar S, Varghese V, Barman P, Ghosh S, Mitra U et al. Changing pattern of human group A rotaviruses: emergence of G12 as an important pathogen among children in eastern India. J Clin Virol 2006; 36:183–188 [View Article]
    [Google Scholar]
  25. Moussa A, Fredj MBH, BenHamida-Rebaï M, Fodha I, Boujaafar N et al. Phylogenetic analysis of partial VP7 gene of the emerging human group A rotavirus G12 strains circulating in Tunisia. J Med Microbiol 2017; 66:112–118 [View Article]
    [Google Scholar]
  26. Chouikha A, Fodha I, Noomen S, Bouzid L, Mastouri M et al. Group A rotavirus strains circulating in the eastern center of Tunisia during a ten-year period (1995-2004). J Med Virol 2007; 79:1002–1008 [View Article]
    [Google Scholar]
  27. Trabelsi A, Fodha I, Chouikha A, Ben Hadj Fredj M, Mastouri M et al. Rotavirus strain diversity in the centre coast of Tunisia from 2000 through 2003. J Infect Dis 2010; 202:S252–S257 [View Article]
    [Google Scholar]
  28. Ben Hadj Fredj M, Zeller M, Fodha I, Heylen E, Chouikha A et al. Molecular characterization of the NSP4 gene of human group A rotavirus strains circulating in Tunisia from 2006 to 2008. Infect Genet Evol 2012; 12:997–1004 [View Article]
    [Google Scholar]
  29. O'Halloran F, Lynch M, Cryan B, O'Shea H, Fanning S. Molecular characterization of rotavirus in Ireland: detection of novel strains circulating in the population. J Clin Microbiol 2000; 38:3370–3374
    [Google Scholar]
  30. Reidy N, O'Halloran F, Fanning S, Cryan B, O'Shea H. Emergence of G3 and G9 rotavirus and increased incidence of mixed infections in the southern region of Ireland 2001-2004. J Med Virol 2005; 77:571–578 [View Article]
    [Google Scholar]
  31. Lennon G, Reidy N, Cryan B, Fanning S, O'Shea H. Changing profile of rotavirus in Ireland: predominance of P[8] and emergence of P[6] and P[9] in mixed infections. J Med Virol 2008; 80:524–530 [View Article]
    [Google Scholar]
  32. Cashman O, Collins PJ, Lennon G, Cryan B, Martella V et al. Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006-2009. Epidemiol Infect 2012; 140:247–259 [View Article]
    [Google Scholar]
  33. Collins PJ, Mulherin E, O'Shea H, Cashman O, Lennon G et al. Changing patterns of rotavirus strains circulating in Ireland: re-emergence of G2P[4] and identification of novel genotypes in Ireland. J Med Virol 2015; 87:764–773 [View Article]
    [Google Scholar]
  34. Gunn L, Feeney SA, Cashman O, Collins PJ, Coyle PV et al. Molecular characterization of group A rotavirus found in elderly patients in Ireland; predominance of G1P[8], continued presence of G9P[8], and emergence of G2P[4]. J Med Virol 2012; 84:2008–2017 [View Article]
    [Google Scholar]
  35. Mouna BH, Hamida-Rebaï MB, Heylen E, Zeller M, Moussa A et al. Sequence and phylogenetic analyses of human rotavirus strains: comparison of VP7 and VP8(∗) antigenic epitopes between Tunisian and vaccine strains before national rotavirus vaccine introduction. Infect Genet Evol 2013; 18:132–144 [View Article]
    [Google Scholar]
  36. Azaran A, Makvandi M, Teimoori A, Ebrahimi S, Heydari F et al. Distribution of rotavirus genotypes Ccirculating in Ahvaz, Iran in 2016. Iran Biomed J 2018; 22:107–116
    [Google Scholar]
  37. Roczo-Farkas S, Kirkwood CD, Bines JE. Commun Dis Intell Q Rep 2017; 41:E455–E71
    [Google Scholar]
  38. Badur S, Öztürk S, Pereira P, AbdelGhany M, Khalaf M et al. Systematic review of the rotavirus infection burden in the WHO-EMRO region. Hum Vaccin Immunother 20191–15 [View Article]
    [Google Scholar]
  39. Gentsch JR, Laird AR, Bielfelt B, Griffin DD, Bányai K et al. Serotype diversity and reassortment between human and animal rotavirus strains: implications for rotavirus vaccine programs. J Infect Dis 2005; 192:S146–S159 [View Article]
    [Google Scholar]
  40. Gentsch JR, Woods PA, Ramachandran M, Das BK, Leite JP et al. Review of G and P typing results from a global collection of rotavirus strains: implications for vaccine development. J Infect Dis 1996; 174:S30–S36 [View Article]
    [Google Scholar]
  41. Armah GE, Pager CT, Asmah RH, Anto FR, Oduro AR et al. Prevalence of unusual human rotavirus strains in Ghanaian children. J Med Virol 2001; 63:67–71 [View Article]
    [Google Scholar]
  42. Gouvea V, Santos N. Rotavirus serotype G5: an emerging cause of epidemic childhood diarrhea. Vaccine 1999; 17:1291–1292
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001031
Loading
/content/journal/jmm/10.1099/jmm.0.001031
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error