RT Journal Article SR Electronic(1) A1 Germ, Julija A1 Cerar Kišek, Tjaša A1 Kokošar Ulčar, Barbara A1 Lejko Zupanc, Tatjana A1 Mrvič, Tatjana A1 Kerin Povšič, Milena A1 Seme, Katja A1 Pirs, MatejaYR 2019 T1 Surveillance cultures for detection of rectal and lower respiratory tract carriage of colistin-resistant Gram-negative bacilli in intensive care unit patients: comparison of direct plating and pre-enrichment step JF Journal of Medical Microbiology, VO 68 IS 9 SP 1269 OP 1278 DO https://doi.org/10.1099/jmm.0.001029 PB Microbiology Society, SN 1473-5644, AB Purpose. Increasing consumption of colistin as treatment for infections with multidrug-resistant (MDR) Gram-negative bacilli (GNB) has been accompanied by increasingly frequent reports of colistin-resistant (ColR) MDR GNB. Higher selective pressure creates a favourable environment that can facilitate the spread of ColR isolates. Monitoring of asymptomatic ColR GNB carriage can give us a better understanding of this emerging healthcare problem, particularly in wards with higher polymyxin selective pressure and prevalence of carbapenem-resistant GNB. Our aim was to assess the ColR GNB colonization rate in intensive care unit (ICU) patients and evaluate the performance of two surveillance protocols using a selective medium. Methodology. A prospective study included 739 surveillance samples (rectal swabs and tracheal aspirates) from 330 patients that were screened for ColR GNB carriage using SuperPolymyxin medium. Two approaches were used: direct sample plating and overnight pre-enrichment of samples followed by plating. Colistin resistance was confirmed with broth microdilution. ColR isolates were molecularly screened for plasmid-mediated mcr genes. Results. A total of 44/739 samples (45 ColR GNB isolates) were positive for ColR GNB, which included 31/330 (9.4 %) colonized patients; mcr genes were not detected. The direct plating method only identified 17/45 (37.8 %) isolates correctly, whereas the pre-enrichment protocol identified all 45 ColR GNB. Conclusion. The colonization rate among our ICU patients was 9.4  %. Based on our findings, the pre-enrichment step is necessary for the determination of ColR GNB carriage – even though the time to result takes an additional day, fewer than half of ColR GNB carriers were detected using the direct plating protocol., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001029