%0 Journal Article %A Osmanov, Ali %A Wise, Andrew %A Denning, David W. %T In vitro and in vivo efficacy of miramistin against drug-resistant fungi %D 2019 %J Journal of Medical Microbiology, %V 68 %N 7 %P 1047-1052 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.001007 %K antifungal %K Aspergillus %K antifungal resistance %K antiseptic %K Candida %I Microbiology Society, %X Introduction. Miramistin is a topical antiseptic with broad antimicrobial activity that was developed in the Soviet Union during the Cold War. Aim. To investigate the antifungal activity of miramistin against clinically relevant drug-resistant fungi. Methodology. The in vitro activity of miramistin was determined following Clinical and Laboratory Standards Institute (CLSI) guidelines. Mammalian cell toxicity was tested using a McCoy cell line and topical and systemic tolerability, and in vivo efficacy was tested using Galleria mellonella models. Results. The minimal inhibitory concentration (MIC) range against fungi was 1.56–25 mg l−1 (GM 3.13 mg l−1 ). In the G. mellonella model, miramistin provided potent survival benefits for Candida albicans and Aspergillus fumigatus infection. Miramistin was tolerated by McCoy cell lines at concentrations up to 1000 mg l−1 and was systemically safe in G. mellonella at 2000 mg kg−1. Topical administration at 32 000 mg l−1 was well tolerated with no adverse effects. Conclusion. These findings support further investigation of miramistin and suggest its possible use for treatment of superficial fungal infections. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001007