1887

Abstract

The aim was to evaluate several microbiological tools for the identification of non-gonococcal spp. isolated from semen samples from Lebanese men and to determine the putative link between the presence of commensal species and infertility.

Within a cross-sectional retrospective study design, the whole population included in this investigation was divided in 2 categories: 173 patients with symptoms of infertility and 139 patients with normal seminograms. Epidemiological and microbiological investigations were performed for 59 strains of through several phenotypic and genotypic tools, including seminograms, an analytical profile index of and (API-NH), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), PCR, 16S rRNA and gene sequencing, and antimicrobial susceptibility testing.

The risk of infection was twice as high in infertile patients compared to the control group [odds ratio (OR): 1.95, confidence interval (CI): 1.05–3.65, =0.03]. Unreliable diagnosis of urogenital infection has serious health and social consequences. Our findings showed that API-NH and 16S rRNA sequencing are poor tools to identify at the species level. Therefore, reliable diagnosis of cases using MALDI-TOF MS and/or sequencing is needed to provide critical treatment decisions and prevent antimicrobial resistance spreading in the community.

This work predicted a strong and significant association between the presence of spp. in semen and male infertility among the Lebanese population. For a better understanding of this association, it is recommended that more genomic and large-scale epidemiological investigations are undertaken to reach definitive conclusions.

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2019-07-01
2024-04-20
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References

  1. Rotman E, Seifert HS. The genetics of Neisseria species. Annu Rev Genet 2014; 48:405–431 [View Article]
    [Google Scholar]
  2. Motamedifar M, Malekzadegan Y, Namdari P, Dehghani B, Jahromi BN et al. The prevalence of bacteriospermia in infertile men and association with semen quality in Southwestern Iran. Infect Disord Drug Targets 2018; 19: [View Article]
    [Google Scholar]
  3. Gerbase AC, Rowley JT, Heymann DH, Berkley SF, Piot P. Global prevalence and incidence estimates of selected curable STDs. Sex Transm Infect 1998; 74:S12–16
    [Google Scholar]
  4. Janda WM, Bohnoff M, Morello JA, Lerner SA. Prevalence and site-pathogen studies of Neisseria meningitidis and N gonorrhoeae in homosexual men. JAMA 1980; 244:2060–2064 [View Article]
    [Google Scholar]
  5. Liu G, Tang CM, Exley RM. Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus. Microbiology 2015; 161:1297–1312 [View Article]
    [Google Scholar]
  6. Hsiao JF, Lee MH, Chia JH, Ho WJ, Chu JJ et al. Neisseria elongata endocarditis complicated by brain embolism and abscess. J Med Microbiol 2008; 57:376–381 [View Article]
    [Google Scholar]
  7. Walker CK, Sweet RL. Gonorrhea infection in women: prevalence, effects, screening, and management. Int J Womens Health 2011; 3:197–206
    [Google Scholar]
  8. Gimenes F, Souza RP, Bento JC, Teixeira JJ, Maria-Engler SS et al. Male infertility: a public health issue caused by sexually transmitted pathogens. Nat Rev Urol 2014; 11:672–687 [View Article]
    [Google Scholar]
  9. Berjis K, Ghiasi M, Sangy S. Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality. Iran J Microbiol 2018; 10:111–116
    [Google Scholar]
  10. La Vignera S, Condorelli RA, Vicari E, Salmeri M, Morgia G et al. Microbiological investigation in male infertility: a practical overview. J Med Microbiol 2014; 63:1–14 [View Article]
    [Google Scholar]
  11. Bennett JS, Jolley KA, Earle SG, Corton C, Bentley SD et al. A genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus Neisseria . Microbiology 2012; 158:1570–1580 [View Article]
    [Google Scholar]
  12. Knapp JS. Historical perspectives and identification of Neisseria and related species. Clin Microbiol Rev 1988; 1:415–431 [View Article]
    [Google Scholar]
  13. Dossett JH, Appelbaum PC, Knapp JS, Totten PA. Proctitis associated with Neisseria cinerea misidentified as Neisseria gonorrhoeae in a child. J Clin Microbiol 1985; 21:575–577
    [Google Scholar]
  14. Ng LK, Martin IE. The laboratory diagnosis of Neisseria gonorrhoeae . Can J Infect Dis Med Microbiol 2005; 16:15–25
    [Google Scholar]
  15. Claesson MJ, Wang Q, O'Sullivan O, Greene-Diniz R, Cole JR et al. Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions. Nucleic Acids Res 2010; 38:e200 [View Article]
    [Google Scholar]
  16. Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010; 16:231–245 [View Article]
    [Google Scholar]
  17. Hjelmevoll SO, Olsen ME, Sollid JU, Haaheim H, Unemo M et al. A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene. J Mol Diagn 2006; 8:574–581 [View Article]
    [Google Scholar]
  18. Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP et al. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 2000; 38:3623–3630
    [Google Scholar]
  19. Yoon SH, SM H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617
    [Google Scholar]
  20. Bennett JS, Watkins ER, Jolley KA, Harrison OB, Maiden MC. Identifying Neisseria species by use of the 50S ribosomal protein L6 (rplF) gene. J Clin Microbiol 2014; 52:1375–1381 [View Article]
    [Google Scholar]
  21. Clinical and Laboratory Standards Institue Document M100-S27 Performance standards for antimicrobial susceptibility testing, twenty -seventh informational supplement. Wayme, PA: CLSI; 2017
    [Google Scholar]
  22. European Committe on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters, version 7.1; 2017
  23. Ehret JM, Nims LJ, Judson FN. A clinical isolate of Neisseria gonorrhoeae with in vitro resistance to erythromycin and decreased susceptibility to azithromycin. Sex Transm Dis 1996; 23:270–272 [View Article]
    [Google Scholar]
  24. Crosnoe LE, Kim ED. Impact of age on male fertility. Curr Opin Obstet Gynecol 2013; 25:181–185 [View Article]
    [Google Scholar]
  25. Whiley DM, Anderson TP, Barratt K, Beaman MH, Buda PJ et al. Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target. Pathology 2006; 38:445–448 [View Article]
    [Google Scholar]
  26. Rosselli R, Romoli O, Vitulo N, Vezzi A, Campanaro S et al. Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon. Sci Rep 2016; 6:32165 [View Article]
    [Google Scholar]
  27. Sellami H, Znazen A, Sellami A, Mnif H, Louati N et al. Molecular detection of Chlamydia trachomatis and other sexually transmitted bacteria in semen of male partners of infertile couples in Tunisia: the effect on semen parameters and spermatozoa apoptosis markers. PLoS One 2014; 9:e98903 [View Article]
    [Google Scholar]
  28. Abusarah EA, Awwad ZM, Charvalos E, Shehabi AA. Molecular detection of potential sexually transmitted pathogens in semen and urine specimens of infertile and fertile males. Diagn Microbiol Infect Dis 2013; 77:283–286 [View Article]
    [Google Scholar]
  29. Berry L, Stanley B. Comparison of self-collected meatal swabs with urine specimens for the diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae in men. J Med Microbiol 2017; 66:134–136 [View Article]
    [Google Scholar]
  30. Whiley DM, Limnios A, Moon NJ, Gehrig N, Goire N et al. False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011. Euro Surveill 2011; 16:
    [Google Scholar]
  31. Buchanan R, Ball D, Dolphin H, Dave J. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the identification of Neisseria gonorrhoeae . Clin Microbiol Infect 2016; 22:815.e5–815.e7 [View Article]
    [Google Scholar]
  32. Kawahara-Matsumizu M, Yamagishi Y, Mikamo H. Misidentification of Neisseria cinerea as Neisseria meningitidis by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Jpn J Infect Dis 2018; 71:85–87 [View Article]
    [Google Scholar]
  33. Ilina EN, Borovskaya AD, Malakhova MM, Vereshchagin VA, Kubanova AA et al. Direct bacterial profiling by matrix-assisted laser Desorption−Ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria . J Mol Diagn 2009; 11:75–86 [View Article]
    [Google Scholar]
  34. Muzzi A, Mora M, Pizza M, Rappuoli R, Donati C. Conservation of meningococcal antigens in the genus Neisseria . mBio 2013; 4:e00163–13 [View Article]
    [Google Scholar]
  35. El Moujaber G, Osman M, Rafei R, Dabboussi F, Hamze M. Molecular mechanisms and epidemiology of resistance in Streptococcus pneumoniae in the Middle East region. J Med Microbiol 2017; 66:847–858 [View Article]
    [Google Scholar]
  36. Jamal S, Al Atrouni A, Rafei R, Dabboussi F, Hamze M et al. Molecular mechanisms of antimicrobial resistance in Acinetobacter baumannii, with a special focus on its epidemiology in Lebanon. J Glob Antimicrob Resist 2018; 15:154–163 [View Article]
    [Google Scholar]
  37. Osman M, Al Mir H, Rafei R, Dabboussi F, Madec JY et al. Epidemiology of antimicrobial resistance in Lebanese extra-hospital settings: an overview. J Glob Antimicrob Resist 2018; 17:123–129 [View Article]
    [Google Scholar]
  38. Moghnieh RA, Kanafani ZA, Tabaja HZ, Sharara SL, Awad LS et al. Epidemiology of common resistant bacterial pathogens in the countries of the Arab League. Lancet Infect Dis 2018; 18:e379–e394 [View Article]
    [Google Scholar]
  39. Kulkarni SV, Bala M, Muqeeth SA, Sasikala G, Nirmalkar AP et al. Antibiotic susceptibility pattern of Neisseria gonorrhoeae strains isolated from five cities in India during 2013–2016. J Med Microbiol 2018; 67:22–28 [View Article]
    [Google Scholar]
  40. Al Omari S, Al Mir H, Wrayde S, Merhabi S, Dhaybi I et al. First antibiotic awareness week nationwide initiative in Lebanon: knowledge, attitude and practice towards antibiotics. J Hosp Infect 2019; 101:475–479
    [Google Scholar]
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