1887

Abstract

and are related ruminant pathogens endemic to different global regions. Potential co-infections combined with the lack of species-specific serological assays challenge accurate diagnosis. Serological screening revealed low seropositivity with the peptide-based ELISA (1/84; 1.2%) in Australian sheep yet moderate seropositivity in a Swiss flock with history of -associated abortions (17/63; 26.9%). By whole cell antigen complement fixation tests (CFT) and ELISA, chlamydial seropositivity was significantly higher in all groups, suggesting cross-reactivity between these two chlamydial species and non-specificity of the tests. However, only DNA could be detected by qPCR in seropositive Australian animals screened, suggesting chlamydial seropositivity was due to cross-reactivity with endemic . These results suggest ascribing seropositivity to chlamydial species in livestock using whole-cell antigen CFT or ELISA should be treated with caution; and that peptide-based ELISA and qPCR provide greater chlamydial species-specificity.

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/content/journal/jmm/10.1099/jmm.0.000951
2019-04-01
2019-12-06
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References

  1. Longbottom D, Coulter LJ. Animal chlamydioses and zoonotic implications. J Comp Pathol 2003;128:217–244 [CrossRef]
    [Google Scholar]
  2. Pospischil A, Thoma R, Hilbe M, Grest P, Gebbers JO. Abortion in woman caused by caprine Chlamydophila abortus (Chlamydia psittaci serovar 1). Swiss Med Wkly 2002;132:64–66
    [Google Scholar]
  3. Animal Health Australia 2016;Animal health in Australia 2015 Canberra, Australia: Animal Health Australia;
  4. West DM. Ovine abortion in New Zealand. N Z Vet J 2002;50:93–95 [CrossRef]
    [Google Scholar]
  5. Seaman JT. Chlamydia isolated from abortion in sheep. Aust Vet J 1985;62:436 [CrossRef]
    [Google Scholar]
  6. McCauley LM, Lancaster MJ, Butler KL, Ainsworth CG. Serological analysis of Chlamydophila abortus in Australian sheep and implications for the rejection of breeder sheep for export. Aust Vet J 2010;88:32–38 [CrossRef]
    [Google Scholar]
  7. Walker E, Lee EJ, Timms P, Polkinghorne A. Chlamydia pecorum infections in sheep and cattle: A common and under-recognised infectious disease with significant impact on animal health. Vet J 2015;206:252–260 [CrossRef]
    [Google Scholar]
  8. Bommana S, Walker E, Desclozeaux M, Timms P, Polkinghorne A. Humoral immune response against two surface antigens of Chlamydia pecorum in vaccinated and naturally infected sheep. PLoS One 2017;12:e0188370–11 [CrossRef]
    [Google Scholar]
  9. McCauley LM, Lancaster MJ, Young P, Butler KL, Ainsworth CG. Comparison of ELISA and CFT assays for Chlamydophila abortus antibodies in ovine sera. Aust Vet J 2007;85:325–328 [CrossRef]
    [Google Scholar]
  10. Vretou E, Radouani F, Psarrou E, Kritikos I, Xylouri E et al. Evaluation of two commercial assays for the detection of Chlamydophila abortus antibodies. Vet Microbiol 2007;123:153–161 [CrossRef]
    [Google Scholar]
  11. Wilson K, Livingstone M, Longbottom D. Comparative evaluation of eight serological assays for diagnosing Chlamydophila abortus infection in sheep. Vet Microbiol 2009;135:38–45 [CrossRef]
    [Google Scholar]
  12. Desclozeaux M, Jelocnik M, Whitting K, Saifzadeh S, Bommana S et al. Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes. Vaccine 2017;35:3461–3465 [CrossRef]
    [Google Scholar]
  13. Walker E, Jelocnik M, Bommana S, Timms P, Carver S et al. Understanding the health and production impacts of endemic Chlamydia pecorum infections in lambs. Vet Microbiol 2018;217:90–96 [CrossRef]
    [Google Scholar]
  14. Marsh J, Kollipara A, Timms P, Polkinghorne A. Novel molecular markers of Chlamydia pecorum genetic diversity in the koala (Phascolarctos cinereus). BMC Microbiology 2011;11:77 [CrossRef]
    [Google Scholar]
  15. Gerber A, Thoma R, Vretou E, Psarrou E, Kaiser C et al. Ovine enzootic abortion (OEA): a comparison of antibody responses in vaccinated and naturally-infected Swiss sheep over a two year period. BMC Vet Res 2007;3:24 [CrossRef]
    [Google Scholar]
  16. Laroucau K, Vorimore F, Bertin C, Yousef Mohamad K, Thierry S et al. Genotyping of Chlamydophila abortus strains by multilocus VNTR analysis. Vet Microbiol 2009;137:335–344 [CrossRef]
    [Google Scholar]
  17. Storz J. Psittacosis-lymphogranuloma infection of sheep: antigenic structures and interrelations of PL agents associated with polyarthritis, enzootic abortion, intrauterine and latent intestinal infections. J Comp Pathol 1966;76:351–362
    [Google Scholar]
  18. Branley J, Bachmann NL, Jelocnik M, Myers GSA, Polkinghorne A. Australian human and parrot Chlamydia psittaci strains cluster within the highly virulent 6BC clade of this important zoonotic pathogen. Scientific Reports 2016;6:30019 [CrossRef]
    [Google Scholar]
  19. Page LA, Cutlip RC. Chlamydia polyarthritis in Iowa lambs. Iowa Veterinarian 1968;39:10–18
    [Google Scholar]
  20. Livingstone M, Entrican G, Wattegedera S, Buxton D, McKendrick IJ et al. Antibody responses to recombinant protein fragments of the major outer membrane protein and polymorphic outer membrane protein POMP90 in Chlamydophila abortus-infected pregnant sheep. Clin Diagn Lab Immunol 2005;12:770–777 [CrossRef]
    [Google Scholar]
  21. Casson N, Entenza JM, Greub G. Serological cross-reactivity between different Chlamydia-like organisms. J Clin Microbiol 2007;45:234–236 [CrossRef]
    [Google Scholar]
  22. Bommana S, Walker E, Desclozeaux M, Jelocnik M, Timms P et al. Molecular and serological dynamics of Chlamydia pecorum infection in a longitudinal study of prime lamb production. PeerJ 2018;6:e4296 [CrossRef]
    [Google Scholar]
  23. Sachse K, Rahman KS, Schnee C, Müller E, Peisker M et al. A novel synthetic peptide microarray assay detects Chlamydia species-specific antibodies in animal and human sera. Sci Rep 2018;8:4701 [CrossRef]
    [Google Scholar]
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