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Volume 68, Issue 4

Clinical and microbial epidemiology of otomycosis in the city of Yasuj, southwest Iran, revealing as the dominant causative agent Free

Abstract

Otomycosis is a mycotic infection of the external auditory canal and can be caused by a wide range of fungal species. In this study, we aimed to identify fungal isolates from patients suspected of otomycosis.

External ear canal samples were taken from patients referred to the outpatient department of Shahid-Mofatteh Clinic in the city of Yasuj, Iran, and examined by direct microscopy and culture. DNA of the isolated fungi was tested by internal transcribed spacer PCR restriction fragment length polymorphism analysis for identification of yeasts and β-tubulin sequencing for identification of species.

Among 275 patients suspected of otomycosis, 144 cases (83 female and 61 male) were confirmed with otomycosis. For 89% (=128) of positive cultures, microscopy was also positive, while there were no cases with a microscopy-positive and culture-negative result. The predominant predisposing factor was self-cleaning of the external ear using unhygienic tools, and the main risk occupation was ‘housewife’. The most common isolated fungi were typically (=120), including 73 isolates of section , 43 of section , 3 of section and 1 of section . After sequencing, 44 out of 73 strains primarily identified as turned out to be . Thirty-five isolates were identified as , including (=22), (=12) and (=1).

was the most common species involved in otomycosis. This work corroborates the difficulty of precise identification of species within the black by morphological characteristics.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aspergillus tubingensis , Candida , housewife , Iran , Otomycosis and Yasuj
© 2019 The Authors
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2019-04-01
2024-04-25
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Clinical and microbial epidemiology of otomycosis in the city of Yasuj, southwest Iran, revealing Aspergillus tubingensis as the dominant causative agent
J. Med. Microbiol. 68, 585 (2019); https://doi.org/10.1099/jmm.0.000948
/content/journal/jmm/10.1099/jmm.0.000948
/content/journal/jmm/10.1099/jmm.0.000948
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