RT Journal Article SR Electronic(1) A1 Rohit, Anusha A1 Suresh Kumar, D. A1 Dhinakaran, I. A1 Joy, Jessy A1 Vijay Kumar, Deekshit A1 Kumar Ballamoole, Krishna A1 Karunasagar, I. A1 Karola, Prior A1 Dag, HarmsenYR 2019 T1 Whole-genome-based analysis reveals multiclone Serratia marcescens outbreaks in a non-Neonatal Intensive Care Unit setting in a tertiary care hospital in India JF Journal of Medical Microbiology, VO 68 IS 4 SP 616 OP 621 DO https://doi.org/10.1099/jmm.0.000947 PB Microbiology Society, SN 1473-5644, AB We report the use of next generation sequencing (NGS) for investigating an outbreak of 13 cases of Serratia marcescens blood stream infections in a non-Neonatal Intensive Care Unit (non-NICU) setting in a tertiary care hospital in India over 5 months. Thirteen cases of sepsis due to S. marcescens were identified in various Intensive Care Units (ICUs) over 5 months. Environmental surveillance identified isolates in the adult ICU (AICU). Antibiogram did not correlate with timeline. Sequencing libraries were prepared using Nextera XT chemistry (Illumina). Based on NGS, two clusters were identified. Cluster 1 had environmental and clinical isolates from the AICU and cluster 2 were isolates from the Coronary Care Unit (CCU). NICU and Paediatric ICU isolates did not belong to any cluster. Polyclonal outbreaks best identified by NGS can occur simultaneously. Good infection prevention practices like hand hygiene for compounded medicines and surface cleaning helped end the outbreak., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000947