%0 Journal Article %A Rohit, Anusha %A Suresh Kumar, D. %A Dhinakaran, I. %A Joy, Jessy %A Vijay Kumar, Deekshit %A Kumar Ballamoole, Krishna %A Karunasagar, I. %A Karola, Prior %A Dag, Harmsen %T Whole-genome-based analysis reveals multiclone Serratia marcescens outbreaks in a non-Neonatal Intensive Care Unit setting in a tertiary care hospital in India %D 2019 %J Journal of Medical Microbiology, %V 68 %N 4 %P 616-621 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000947 %K next generation sequencing %K outbreak investigation %K non-NICU Serratia marcescens %I Microbiology Society, %X We report the use of next generation sequencing (NGS) for investigating an outbreak of 13 cases of Serratia marcescens blood stream infections in a non-Neonatal Intensive Care Unit (non-NICU) setting in a tertiary care hospital in India over 5 months. Thirteen cases of sepsis due to S. marcescens were identified in various Intensive Care Units (ICUs) over 5 months. Environmental surveillance identified isolates in the adult ICU (AICU). Antibiogram did not correlate with timeline. Sequencing libraries were prepared using Nextera XT chemistry (Illumina). Based on NGS, two clusters were identified. Cluster 1 had environmental and clinical isolates from the AICU and cluster 2 were isolates from the Coronary Care Unit (CCU). NICU and Paediatric ICU isolates did not belong to any cluster. Polyclonal outbreaks best identified by NGS can occur simultaneously. Good infection prevention practices like hand hygiene for compounded medicines and surface cleaning helped end the outbreak. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000947