1887

Journal of Medical Microbiology logo

Volume 68, Issue 3

In vitro activities of daptomycin combined with fosfomycin or rifampin on planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis Free

Abstract

Purpose. This study aimed to explore daptomycin combined with fosfomycin or rifampin against the planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis .

Methodology. Four linezolid-resistant and four linezolid-sensitive isolates of E. faecalis which formed biofilms were collected for this study. Biofilm biomasses were detected by crystal violet staining and the adherent cells in the mature biofilms were quantified by c.f.u. determination.

Results. Daptomycin alone, or combined with fosfomycin or rifampin (4×MIC) demonstrated bactericidal activities on the planktonic cells, and daptomycin combined with fosfomycin killed more planktonic cells (at least 1−log10 c.f.u. ml) than daptomycin or fosfomycin alone. Daptomycin alone (16×MIC) showed anti-biofilm activities against the mature biofilms and bactericidal activities on the adherent cells, while daptomycin combined with fosfomycin (16×MIC) demonstrated significantly more anti-biofilm activities than daptomycin or fosfomycin alone and effectively killed the adherent cells in the mature biofilms. The high concentration of daptomycin (512 mg l ) combined with fosfomycin indicated more bactericidal activities on the adherent cells and more anti-biofilm activities against the mature biofilms than daptomycin 64 mg l (16×MIC) combined with fosfomycin. The addition of rifampin increased the anti-biofilm and bactericidal activities of daptomycin against the mature biofilms and the adherent cells of two isolates, however, which was not observed in other isolates.

Conclusions. Daptomycin combined with fosfomycin demonstrated better effect on the planktonic and adherent linezolid-resistant isolates of E. faecalis than daptomycin or fosfomycin alone. The role of rifampin in the treatment of E. faecalis isolates is discrepant and needs more studies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , daptomycin , Enterococcus faecalis , fosfomycin , linezolid-resistant and rifampin
© 2019 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000945
2019-03-14
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/68/3/493.html?itemId=/content/journal/jmm/10.1099/jmm.0.000945&mimeType=html&fmt=ahah

References

  1. Beganovic M, Luther MK, Rice LB, Arias CA, Rybak MJ et al. A review of combination antimicrobial therapy for Enterococcusfaecalis bloodstream infections and infective endocarditis. Clin Infect Dis 2018; 67:303–309 [View Article]
     [Google Scholar]
  2. Flokas ME, Karageorgos SA, Detsis M, Alevizakos M, Mylonakis E. Vancomycin-resistant enterococci colonisation, risk factors and risk for infection among hospitalised paediatric patients: a systematic review and meta-analysis. Int J Antimicrob Agents 2017; 49:565–572 [View Article]
     [Google Scholar]
  3. Meka VG, Gold HS. Antimicrobial resistance to linezolid. Clin Infect Dis 2004; 39:1010–1015 [View Article]
     [Google Scholar]
  4. Zheng JX, Wu Y, Lin ZW, Pu ZY, Yao WM et al. Characteristics of and virulence factors associated with biofilm formation in clinical Enterococcus faecalis isolates in China. Front Microbiol 2017; 8:2338 [View Article]
     [Google Scholar]
  5. Cai J, Wang Y, Schwarz S, Lv H, Li Y et al. Enterococcal isolates carrying the novel oxazolidinone resistance gene optrA from hospitals in Zhejiang, Guangdong, and Henan, China, 2010–2014. Clin Microbiol Infect 2015; 21:1095.e1–4 [View Article]
     [Google Scholar]
  6. Lazaris A, Coleman DC, Kearns AM, Pichon B, Kinnevey PM et al. Novel multiresistance cfr plasmids in linezolid-resistant methicillin-resistant Staphylococcus epidermidis and vancomycin-resistant Enterococcus faecium (VRE) from a hospital outbreak: co-location of cfr and optrA in VRE. J Antimicrob Chemother 2017; 72:3252–3257 [View Article]
     [Google Scholar]
  7. Pfaller MA, Mendes RE, Streit JM, Hogan PA, Flamm RK. Five-year summary of in Vitro activity and resistance mechanisms of linezolid against clinically important gram-positive cocci in the United States from the LEADER surveillance program (2011 to 2015). Antimicrob Agents Chemother 2017; 61: pii e00609–00617 [View Article]
     [Google Scholar]
  8. Sandoe JA, Witherden IR, Cove JH, Heritage J, Wilcox MH. Correlation between enterococcal biofilm formation in vitro and medical-device-related infection potential in vivo. J Med Microbiol 2003; 52:547–550 [View Article]
     [Google Scholar]
  9. Seno Y, Kariyama R, Mitsuhata R, Monden K, Kumon H. Clinical implications of biofilm formation by Enterococcus faecalis in the urinary tract. Acta Med Okayama 2005; 59:79–87 [View Article]
     [Google Scholar]
  10. Toledo-Arana A, Valle J, Solano C, Arrizubieta MJ, Cucarella C et al. The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation. Appl Environ Microbiol 2001; 67:4538–4545 [View Article]
     [Google Scholar]
  11. Duprè I, Zanetti S, Schito AM, Fadda G, Sechi LA. Incidence of virulence determinants in clinical Enterococcus faecium and Enterococcus faecalis isolates collected in Sardinia (Italy). J Med Microbiol 2003; 52:491–498 [View Article]
     [Google Scholar]
  12. Mohamed JA, Huang W, Nallapareddy SR, Teng F, Murray BE. Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis. Infect Immun 2004; 72:3658–3663 [View Article]
     [Google Scholar]
  13. Lewis K. Riddle of biofilm resistance. Antimicrob Agents Chemother 2001; 45:999–1007 [View Article]
     [Google Scholar]
  14. Luther MK, Arvanitis M, Mylonakis E, LaPlante KL. Activity of daptomycin or linezolid in combination with rifampin or Gentamicin against biofilm-forming Enterococcus faecalis or E. faecium in an in vitro pharmacodynamic model using simulated endocardial vegetations and an in vivo survival assay using Galleria mellonella larvae. Antimicrob Agents Chemother 2014; 58:4612–4620 [View Article]
     [Google Scholar]
  15. Oliva A, Furustrand Tafin U, Maiolo EM, Jeddari S, Bétrisey B et al. Activities of fosfomycin and rifampin on planktonic and adherent Enterococcus faecalis strains in an experimental foreign-body infection model. Antimicrob Agents Chemother 2014; 58:1284–1293 [View Article]
     [Google Scholar]
  16. Tang HJ, Chen CC, Zhang CC, Su BA, Li CM et al. In vitro efficacy of fosfomycin-based combinations against clinical vancomycin-resistant Enterococcus isolates. Diagn Microbiol Infect Dis 2013; 77:254–257 [View Article]
     [Google Scholar]
  17. Wang C, Li M, Dong D, Wang J, Ren J et al. Role of ClpP in biofilm formation and virulence of Staphylococcus epidermidis. Microbes Infect 2007; 9:1376–1383 [View Article]
     [Google Scholar]
  18. Zhang QZ, Zhao KQ, Wu Y, Li XH, Yang C et al. 5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm. PLoS One 2017; 12:e0174627 [View Article]
     [Google Scholar]
  19. Rice LB, Eliopoulos GM, Moellering RC Jr. In vitro synergism between daptomycin and fosfomycin against Enterococcus faecalis isolates with high-level gentamicin resistance. Antimicrob Agents Chemother 1989; 33:470–473 [View Article]
     [Google Scholar]
  20. Descourouez JL, Jorgenson MR, Wergin JE, Rose WE. Fosfomycin synergy in vitro with amoxicillin, daptomycin, and linezolid against vancomycin-resistant Enterococcus faecium from renal transplant patients with infected urinary stents. Antimicrob Agents Chemother 2013; 57:1518–1520 [View Article]
     [Google Scholar]
  21. Hall Snyder AD, Werth BJ, Nonejuie P, McRoberts JP, Pogliano J et al. Fosfomycin enhances the activity of daptomycin against vancomycin-resistant enterococci in an in vitro pharmacokinetic-pharmacodynamic model. Antimicrob Agents Chemother 2016; 60:5716–5723 [View Article]
     [Google Scholar]
  22. Miró JM, Entenza JM, Del Río A, Velasco M, Castañeda X et al. High-dose daptomycin plus fosfomycin is safe and effective in treating methicillin-susceptible and methicillin-resistant Staphylococcus aureus endocarditis. Antimicrob Agents Chemother 2012; 56:4511–4515 [View Article]
     [Google Scholar]
  23. Kullar R, Casapao AM, Davis SL, Levine DP, Zhao JJ et al. A multicentre evaluation of the effectiveness and safety of high-dose daptomycin for the treatment of infective endocarditis. J Antimicrob Chemother 2013; 68:2921–2926 [View Article]
     [Google Scholar]
  24. Mikuniya T, Kato Y, Ida T, Maebashi K, Monden K et al. Treatment of Pseudomonas aeruginosa biofilms with a combination of fluoroquinolones and fosfomycin in a rat urinary tract infection model. J Infect Chemother 2007; 13:285–290 [View Article]
     [Google Scholar]
  25. Corvec S, Furustrand Tafin U, Betrisey B, Borens O, Trampuz A. Activities of fosfomycin, tigecycline, colistin, and Gentamicin against extended-spectrum-β-lactamase-producing Escherichia coli in a foreign-body infection model. Antimicrob Agents Chemother 2013; 57:1421–1427 [View Article]
     [Google Scholar]
  26. Garrigós C, Murillo O, Lora-Tamayo J, Verdaguer R, Tubau F et al. Fosfomycin-daptomycin and other fosfomycin combinations as alternative therapies in experimental foreign-body infection by methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2013; 57:606–610 [View Article]
     [Google Scholar]
  27. Raz R. Fosfomycin: an old-new antibiotic. Clin Microbiol Infect 2012; 18:4–7 [View Article]
     [Google Scholar]
  28. Zimmerli W, Widmer AF, Blatter M, Frei R, Ochsner PE. Role of rifampin for treatment of orthopedic implant-related staphylococcal infections: a randomized controlled trial. foreign-body infection (FBI) Study Group. JAMA 1998; 279:1537–1541
     [Google Scholar]
  29. Baldoni D, Haschke M, Rajacic Z, Zimmerli W, Trampuz A. Linezolid alone or combined with rifampin against methicillin-resistant Staphylococcus aureus in experimental foreign-body infection. Antimicrob Agents Chemother 2009; 53:1142–1148 [View Article]
     [Google Scholar]
  30. Vergidis P, Rouse MS, Euba G, Karau MJ, Schmidt SM et al. Treatment with linezolid or vancomycin in combination with rifampin is effective in an animal model of methicillin-resistant Staphylococcus aureus foreign body osteomyelitis. Antimicrob Agents Chemother 2011; 55:1182–1186 [View Article]
     [Google Scholar]
  31. Holmberg A, Mörgelin M, Rasmussen M. Effectiveness of ciprofloxacin or linezolid in combination with rifampicin against Enterococcus faecalis in biofilms. J Antimicrob Chemother 2012; 67:433–439 [View Article]
     [Google Scholar]
  32. Minardi D, Cirioni O, Ghiselli R, Silvestri C, Mocchegiani F et al. Efficacy of tigecycline and rifampin alone and in combination against Enterococcus faecalis biofilm infection in a rat model of ureteral stent. J Surg Res 2012; 176:1–6 [View Article]
     [Google Scholar]
  33. Erskine E, MacPhee CE, Stanley-Wall NR. Functional amyloid and other protein fibers in the biofilm matrix. J Mol Biol 2018; 430:3642–3656 [View Article]
     [Google Scholar]
  34. Koo H, Yamada KM. Dynamic cell-matrix interactions modulate microbial biofilm and tissue 3D microenvironments. Curr Opin Cell Biol 2016; 42:102–112 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000945
Loading
In vitro activities of daptomycin combined with fosfomycin or rifampin on planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis
J. Med. Microbiol. 68, 493 (2019); https://doi.org/10.1099/jmm.0.000945
/content/journal/jmm/10.1099/jmm.0.000945
/content/journal/jmm/10.1099/jmm.0.000945
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error