Performance of bioMérieux Lowenstein–Jensen slopes in plastic tube packaging, compared to existing phenotypic methods, for efficient recovery of the Mycobacterium tuberculosis complex
Purpose. Lowenstein–Jensen (LJ) medium used for cultivating Mycobacterium tuberculosis (MTB) is marketed in glass packaging. Breakage of glass slope is a major biosafety risk, especially during processing and storage, which gets magnified in large laboratories. We evaluated the performance of new bioMérieux (bMx) LJ slopes in plastic packaging, compared to bMx glass LJ medium and Becton Dickinson Mycobacterial Growth Indicator Tube (MGIT), for MTB recovery.
Methodology. Consecutive pulmonary/extra-pulmonary samples (n=240) were processed using routine methods of decontamination, inoculation and incubation.
Results. Plastic LJ slopes detected all 213 true-positive cases. The mean time-to-growth detection was 17.97 days for plastic LJ slopes, compared to 18.08 and 13.53 days for glass LJ slopes and MGIT, respectively. No statistically significant difference was observed between the two LJ slopes (P< 0.05). Both LJ slopes had a sensitivity and specificity of 100%, with respect to MGIT.
Conclusion. Plastic LJ slopes are a good alternative to the traditional glass slopes. The medium quality did not differ with the packaging material. Increased surface area of these slants allowed enhanced growth, and the clear plastic material allowed accurate recording of growth. The wide mouth of these containers eased inoculation. Increased biosafety, by elimination of breakage risk, is the biggest advantage of this modification.
KentPT, KubicaGP. Department of Health and Human Services, Public Health Services, Center for Disease Control. Public Health Mycobacteriology: a Guide for Level III Lab Atlanta, GA: CDC; 1985
NambiarR, ChatellierS, BereksiN, van BelkumA, SinghN et al. Evaluation of Mycotube, a modified version of Lowenstein–Jensen (LJ) Medium, for efficient recovery of Mycobacterium tuberculosis (Mtb). Eur J Clin Microbiol Infect Dis2017; 36:1981–1988 [View Article]
EssaSA, Abdel-SameaSAR, IsmaeilYM, MohammadAA. Comparative study between using Lowenstein–Jensen and Bio-FM media in identification of Mycobacterium tuberculosis. Egypt J Chest Dis Tuberc2013; 62:249–255 [View Article]
TortoliE, CicheroP, PiersimoniC, SimonettiMT, GesuG et al. Use of BACTEC MGIT 960 for recovery of mycobacteria from clinical specimens: multicenter study. J Clin Microbiol1999; 37:3578–3582
American Thoracic Society Diagnostic standards and classification of tuberculosis in adults and children. Am J Respir Crit Care Med2000; 161:1376–1395 [View Article]
RodriguesC, ShenaiS, SadaniM, SukhadiaN, JaniM et al. Evaluation of the Bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high through put tertiary care centre. Indian J Med Microbiol2009; 27:217–221 [View Article]
ChihotaVN, GrantAD, FieldingK, NdibongoB, van ZylA et al. Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting. Int J Tuberc Lung Dis2010; 14:1024–1031
RageadeF, PicotN, Blanc-MichaudA, ChatellierS, MirandeC et al. Performance of solid and liquid culture media for the detection of Mycobacterium tuberculosis in clinical materials: meta-analysis of recent studies. Eur J Clin Microbiol Infect Dis2014; 33:867–870 [View Article]
Performance of bioMérieux Lowenstein–Jensen slopes in plastic tube packaging, compared to existing phenotypic methods, for efficient recovery of the Mycobacterium tuberculosis complex