1887

Abstract

A multidrug-resistant Pseudomonas aeruginosa PS1 isolated from urine clinical sample was investigated in this study. The strain exhibited resistance to piperacillin/tazobactam, ciprofloxacin, imipenem, ceftazidime but it was susceptible to colistin. Analysis of whole-genome sequencing data by ResFinder detected various resistance determinants including qnrVC1 and bla NDM-1. The multiresistant P. aeruginosa isolate belonged to ST773 high-risk clone. The qnrVC1 and bla NDM-1 determinants were incorporated into different integrons. Expression of bla NDM-1 was fourfold and qnrVC1 was twofold increased, compared to that of rpsL housekeeping gene. Mutations in gyrA Thr83Leu and parC Ser87Leu were detected and additionally qnrVC1 expression indicates protective effect of QnrVC1 pentapeptid protein on gyrase and topoisomerase. High-risk P. aeruginosa clones integrate various carbapenemase and other resistance determinants into their genomes that facilitates further dissemination of multiresistance among clinical isolates. We report bla NDM-1 and qnrVC1 genes in P. aeruginosa ST773 international high-risk clone.

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2019-01-22
2024-04-16
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References

  1. Bonomo RA, Szabo D. Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa. Clin Infect Dis 2006; 43:S49–S56 [View Article]
    [Google Scholar]
  2. Laudy AE, Róg P, Smolińska-Król K, Ćmiel M, Słoczyńska A et al. Prevalence of ESBL-producing Pseudomonas aeruginosa isolates in Warsaw, Poland, detected by various phenotypic and genotypic methods. PLoS One 2017; 12:e0180121 [View Article]
    [Google Scholar]
  3. Cornaglia G, Giamarellou H, Rossolini GM. Metallo-β-lactamases: a last frontier for β-lactams?. Lancet Infect Dis 2011; 11:381–393 [View Article]
    [Google Scholar]
  4. Rodríguez-Martínez JM, Machuca J, Cano ME, Calvo J, Martínez-Martínez L et al. Plasmid-mediated quinolone resistance: two decades on. Drug Res Update 2016; 29:13–29 [View Article]
    [Google Scholar]
  5. Fonseca EL, dos Santos Freitas F, Vieira VV, Vicente ACP. New qnr gene cassettes associated with superintegron repeats in Vibrio cholerae O1. Emerg Infect Dis 2008; 14:1129–1131 [View Article]
    [Google Scholar]
  6. Fonseca EL, Vicente ACP. Epidemiology of qnrVC alleles and emergence out of the Vibrionaceae family. J Med Microbiol 2013; 62:1628–1630 [View Article]
    [Google Scholar]
  7. Belotti PT, Thabet L, Laffargue A, André C, Coulange-Mayonnove L et al. Description of an original integron encompassing blaVIM-2, qnrVC1 and genes encoding bacterial group II intron proteins in Pseudomonas aeruginosa. J Antimicrob Chemother 2015; 70:2237–2240 [View Article]
    [Google Scholar]
  8. Heang V, Hout B, Prouty MG, Supraprom C, Ford GW et al. Detection of qnrVC and rmtB genes from a multidrug-resistant Ralstonia pickettii wound infection isolate in Cambodia. Int J Antimicrob Agents 2014; 44:84–85 [View Article]
    [Google Scholar]
  9. Wright LL, Turton JF, Livermore DM, Hopkins KL, Woodford N. Dominance of international 'high-risk clones' among metallo-β-lactamase-producing Pseudomonas aeruginosa in the UK. J Antimicrob Chemother 2015; 70:103–110 [View Article]
    [Google Scholar]
  10. Woodford N, Turton JF, Livermore DM. Multiresistant Gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance. FEMS Microbiol Rev 2011; 35:736–755 [View Article]
    [Google Scholar]
  11. Edelstein MV, Skleenova EN, Shevchenko OV, D'souza JW, Tapalski DV et al. Spread of extensively resistant VIM-2-positive ST235 Pseudomonas aeruginosa in Belarus, Kazakhstan, and Russia: a longitudinal epidemiological and clinical study. Lancet Infect Dis 2013; 13:867–876 [View Article]
    [Google Scholar]
  12. Silva FM, Carmo MS, Silbert S, Gales AC. SPM-1-producing Pseudomonas aeruginosa: analysis of the ancestor relationship using multilocus sequence typing, pulsed-field gel electrophoresis, and automated ribotyping. Microb Drug Resist 2011; 17:215–220 [View Article]
    [Google Scholar]
  13. Saiman L, Burns JL, Whittier S, Krzewinski J, Marshall SA et al. Evaluation of reference dilution test methods for antimicrobial susceptibility testing of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis. J Clin Microbiol 1999; 37:2987–2991
    [Google Scholar]
  14. Lindsey RL, Pouseele H, Chen JC, Strockbine NA, Carleton HA. Implementation of whole genome sequencing (WGS) for identification and characterization of shiga toxin-producing Escherichia coli (STEC) in the United States. Front Microbiol 2016; 7:766 [View Article]
    [Google Scholar]
  15. Watts JEM, Schreier HJ, Lanska L, Hale MS. The rising tide of antimicrobial resistance in aquaculture: sources, sinks and solutions. Mar Drugs 2017; 15:158 [View Article]
    [Google Scholar]
  16. da Fonseca ÉL, Vicente ACP. Functional characterization of a Cassette-specific promoter in the class 1 integron-associated qnrVC1 gene. Antimicrob Agents Chemother 2012; 56:3392–3394 [View Article]
    [Google Scholar]
  17. Ellington MJ, Woodford N. Fluoroquinolone resistance and plasmid addiction systems: self-imposed selection pressure?. J Antimicrob Chemother 2006; 57:1026–1029 [View Article]
    [Google Scholar]
  18. Libisch B, Giske CG, Kovács B, Tóth TG, Füzi M. Identification of the first VIM metallo-beta-lactamase-producing multiresistant Aeromonas hydrophila strain. J Clin Microbiol 2008; 46:1878–1880 [View Article]
    [Google Scholar]
  19. Libisch B, Muzslay M, Gacs M, Minárovits J, Knausz M et al. Molecular epidemiology of VIM-4 metallo-beta-lactamase-producing Pseudomonas sp. isolates in Hungary. Antimicrob Agents Chemother 2006; 50:4220–4223 [View Article]
    [Google Scholar]
  20. Kristóf K, Tóth A, Damjanova I, Jánvári L, Konkoly-Thege M et al. Identification of a blaVIM-4 gene in the internationally successful Klebsiella pneumoniae ST11 clone and in a Klebsiella oxytoca strain in Hungary. J Antimicrob Chemother 2010; 65:1303–1305 [View Article]
    [Google Scholar]
  21. Yousefi S, Nahaei MR, Farajnia S, Aghazadeh M, Iversen A et al. A multiresistant clone of Pseudomonas aeruginosa sequence type 773 spreading in a burn unit in Orumieh, Iran. APMIS 2013; 121:146–152 [View Article]
    [Google Scholar]
  22. Kim MJ, Bae IK, Jeong SH, Kim SH, Song JH et al. Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. J Antimicrob Chemother 2013; 68:2820–2824 [View Article]
    [Google Scholar]
  23. Tada T, Nhung PH, Miyoshi-Akiyama T, Shimada K, Tsuchiya M et al. Multidrug-resistant sequence type 235 Pseudomonas aeruginosa clinical isolates producing IMP-26 with increased carbapenem-hydrolyzing activities in Vietnam. Antimicrob Agents Chemother 2016; 60:6853–6858 [View Article]
    [Google Scholar]
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