1887

Abstract

Intra-familial infection, mother-to-child infection, is considered to be one of the main routes of transmission for in developed countries such as Japan. A major role for intra-familial spread in the pathogenicity of is now beyond controversy, although the major route of transmission remains poorly understood. We performed this study to clarify the factors determining intra-familial transmission.

We used several strains isolated from family members to compare infectivity. K21 and K22 strains were isolated from the father and mother, and the K25 strain was isolated from the third child of the family. Mongolian gerbils were inoculated with strains and the infectivity of three strains was compared in each experiment. In addition, the whole genome sequence, adhesion to gastric epithelial cells and the growth of static condition or continuous flow culture among three strains of were analysed.

Most of the colonies were determined as the same molecular type K25 in all of the four grouped animals and K25 was observed as the dominant strain. The stronger adhesion capacity of the K25 strain was observed in comparison with the other two strains through analysis. By assessing the genomic profiles of isolates from three strains, identified TnPZ regions were detected only in the K25 strain.

The infectivity of isolates intra-familial infection and animal infection were prescribed by the adhesion capacity and molecular type of each strain.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000918
2019-04-01
2019-10-21
Loading full text...

Full text loading...

References

  1. Suerbaum S, Michetti P. Helicobacter pylori infection. N Engl J Med 2002;347:1175–1186 [CrossRef]
    [Google Scholar]
  2. Asaka M. Helicobacter pylori infection and gastric cancer. Intern Med 2002;41:1–6 [CrossRef]
    [Google Scholar]
  3. Myint T, Shiota S, Vilaichone RK, Ni N, Aye TT et al. Prevalence of Helicobacter pylori infection and atrophic gastritis in patients with dyspeptic symptoms in Myanmar. World J Gastroenterol 2015;21:629–636 [CrossRef]
    [Google Scholar]
  4. Asaka M, Kato M, Kudo M, Meguro T, Kimura T et al. The role of Helicobacter pylori in peptic ulcer disease. Gastroenterol Jpn 1993;28:163–167 [CrossRef]
    [Google Scholar]
  5. Yucel O. Prevention of Helicobacter pylori infection in childhood. World J Gastroenterol 2014;20:10348–10354 [CrossRef]
    [Google Scholar]
  6. Roosendaal R, Kuipers EJ, Buitenwerf J, van Uffelen C, Meuwissen SG et al. Helicobacter pylori and the birth cohort effect: evidence of a continuous decrease of infection rates in childhood. Am J Gastroenterol 1997;92:1480–1482
    [Google Scholar]
  7. Kurosawa M, Kikuchi S, Inaba Y, Ishibashi T, Kobayashi F. Helicobacter pylori infection among Japanese children. J Gastroenterol Hepatol 2000;15:1382–1385 [CrossRef]
    [Google Scholar]
  8. Kivi M, Tindberg Y. Helicobacter pylori occurrence and transmission: a family affair?. Scand J Infect Dis 2006;38:407–417 [CrossRef]
    [Google Scholar]
  9. Konno M, Fujii N, Yokota S, Sato K, Takahashi M et al. Five-year follow-up study of mother-to-child transmission of Helicobacter pylori infection detected by a random amplified polymorphic DNA fingerprinting method. J Clin Microbiol 2005;43:2246–2250 [CrossRef]
    [Google Scholar]
  10. Parsonnet J, Shmuely H, Haggerty T. Fecal and oral shedding of Helicobacter pylori from healthy infected adults. Jama 1999;282:2240–2245
    [Google Scholar]
  11. Allaker RP, Young KA, Hardie JM, Domizio P, Meadows NJ. Prevalence of Helicobacter pylori at oral and gastrointestinal sites in children: evidence for possible oral-to-oral transmission. J Med Microbiol 2002;51:312–317 [CrossRef]
    [Google Scholar]
  12. Fujimura S, Kato S, Watanabe A. Water source as a Helicobacter pylori transmission route: a 3-year follow-up study of Japanese children living in a unique district. J Med Microbiol 2008;57:909–910 [CrossRef]
    [Google Scholar]
  13. Goh KL, Chan WK, Shiota S, Yamaoka Y. Epidemiology of Helicobacter pylori infection and public health implications. Helicobacter 2011;16:1–9 [CrossRef]
    [Google Scholar]
  14. Fialho AM, Braga AB, Braga Neto MB, Carneiro JG, Rocha AM et al. Younger siblings play a major role in Helicobacter pylori transmission among children from a low-income community in the Northeast of Brazil. Helicobacter 2010;15:491–496 [CrossRef]
    [Google Scholar]
  15. Weyermann M, Rothenbacher D, Brenner H. Acquisition of Helicobacter pylori infection in early childhood: independent contributions of infected mothers, fathers and siblings. Am J Gastroenterol 2009;104:182–189 [CrossRef]
    [Google Scholar]
  16. Malaty HM, Paykov V, Bykova O, Ross A, Graham DP et al. Helicobacter pylori and socioeconomic factors in Russia. Helicobacter 1996;1:82–87 [CrossRef]
    [Google Scholar]
  17. Garg PK, Perry S, Sanchez L, Parsonnet J. Concordance of Helicobacter pylori infection among children in extended-family homes. Epidemiol Infect 2006;134:450–459 [CrossRef]
    [Google Scholar]
  18. Fiedorek SC, Malaty HM, Evans DL, Pumphrey CL, Casteel HB et al. Factors influencing the epidemiology of Helicobacter pylori infection in children. Pediatrics 1991;88:578–582
    [Google Scholar]
  19. Goodman KJ, Correa P. Transmission of Helicobacter pylori among siblings. Lancet 2000;355:358–362 [CrossRef]
    [Google Scholar]
  20. Osaki T, Konno M, Yonezawa H, Hojo F, Zaman C et al. Analysis of intra-familial transmission of Helicobacter pylori in Japanese families. J Med Microbiol 2015;64:67–73 [CrossRef]
    [Google Scholar]
  21. Furuta Y, Konno M, Osaki T, Yonezawa H, Ishige T et al. Microevolution of virulence-related genes in Helicobacter pylori familial infection. PLoS One 2015;10:e0127197 [CrossRef]
    [Google Scholar]
  22. Kersulyte D, Lee W, Subramaniam D, Anant S, Herrera P et al. Helicobacter Pylori's plasticity zones are novel transposable elements. PLoS One 2009;4:e6859 [CrossRef]
    [Google Scholar]
  23. Zaman C, Osaki T, Hanawa T, Yonezawa H, Kurata S et al. Analysis of the microbial ecology between Helicobacter pylori and the gastric microbiota of Mongolian gerbils. J Med Microbiol 2014;63:129–137 [CrossRef]
    [Google Scholar]
  24. Oshio I, Osaki T, Hanawa T, Yonezawa H, Zaman C et al. Vertical Helicobacter pylori transmission from Mongolian gerbil mothers to pups. J Med Microbiol 2009;58:656–662 [CrossRef]
    [Google Scholar]
  25. Osaki T, Matsuki T, Asahara T, Zaman C, Hanawa T et al. Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori. Microb Pathog 2012;53:12–18 [CrossRef]
    [Google Scholar]
  26. Konno M, Yokota S, Suga T, Takahashi M, Sato K et al. Predominance of mother-to-child transmission of Helicobacter pylori infection detected by random amplified polymorphic DNA fingerprinting analysis in Japanese families. Pediatr Infect Dis J 2008;27:999–1003 [CrossRef]
    [Google Scholar]
  27. Akopyanz N, Bukanov NO, Westblom TU, Kresovich S, Berg DE. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 1992;20:5137–5142 [CrossRef]
    [Google Scholar]
  28. Rinttilä T, Kassinen A, Malinen E, Krogius L, Palva A. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 2004;97:1166–1177 [CrossRef]
    [Google Scholar]
  29. Jolley KA, Maiden MC. BIGSdb: scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 2010;11:595 [CrossRef]
    [Google Scholar]
  30. Kotzamanidis C, Kourelis A, Litopoulou-Tzanetaki E, Tzanetakis N, Yiangou M. Evaluation of adhesion capacity, cell surface traits and immunomodulatory activity of presumptive probiotic Lactobacillus strains. Int J Food Microbiol 2010;140:154–163 [CrossRef]
    [Google Scholar]
  31. Kobayashi Y, Okazaki K, Murakami K. Adhesion of Helicobacter pylori to gastric epithelial cells in primary cultures obtained from stomachs of various animals. Infect Immun 1993;61:4058–4063
    [Google Scholar]
  32. Jackman SD, Vandervalk BP, Mohamadi H, Chu J, Yeo S et al. ABySS 2.0: resource-efficient assembly of large genomes using a bloom filter. Genome Res 2017;27:768–777 [CrossRef]
    [Google Scholar]
  33. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014;30:2068–2069 [CrossRef]
    [Google Scholar]
  34. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 2015;31:3691–3693 [CrossRef]
    [Google Scholar]
  35. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403–410 [CrossRef]
    [Google Scholar]
  36. Lai CH, Huang JC, Chiang-Ni C, Li JP, Wu LT et al. Mixed infections of Helicobacter pylori isolated from patients with gastrointestinal diseases in Taiwan. Gastroenterol Res Pract 2016;2016:1–5 [CrossRef]
    [Google Scholar]
  37. Matsuda K, Yamauchi K, Matsumoto T, Sano K, Yamaoka Y et al. Quantitative analysis of the effect of Helicobacter pylori on the expressions of SOX2, CDX2, MUC2, MUC5AC, MUC6, TFF1, TFF2, and TFF3 mRNAs in human gastric carcinoma cells. Scand J Gastroenterol 2008;43:25–33
    [Google Scholar]
  38. Solnick JV, Hansen LM, Salama NR, Boonjakuakul JK, Syvanen M. Modification of Helicobacter pylori outer membrane protein expression during experimental infection of rhesus macaques. Proc Natl Acad Sci USA 2004;101:2106–2111 [CrossRef]
    [Google Scholar]
  39. Tan S, Tompkins LS, Amieva MR. Helicobacter pylori usurps cell polarity to turn the cell surface into a replicative niche. PLoS Pathogens 2009;5:e1000407 [CrossRef]
    [Google Scholar]
  40. Oleastro M, Ménard A. The role of Helicobacter pylori outer membrane proteins in adherence and pathogenesis. Biology 2013;2:1110–1134 [CrossRef]
    [Google Scholar]
  41. McGee DJ, Langford ML, Watson EL, Carter JE, Chen YT et al. Colonization and inflammation deficiencies in Mongolian gerbils infected by Helicobacter pylori chemotaxis mutants. Infect Immun 2005;73:1820–1827 [CrossRef]
    [Google Scholar]
  42. Yamaoka Y, Ojo O, Fujimoto S, Odenbreit S, Haas R et al. Helicobacter pylori outer membrane proteins and gastroduodenal disease. Gut 2006;55:775–781 [CrossRef]
    [Google Scholar]
  43. Sugimoto M, Ohno T, Graham DY, Yamaoka Y. Gastric mucosal interleukin-17 and -18 mRNA expression in Helicobacter pylori-induced Mongolian gerbils. Cancer Sci 2009;100:2152–2159 [CrossRef]
    [Google Scholar]
  44. Zhong Q, Shao SH, Cui LL, Mu RH, Ju XL et al. Type IV secretion system in Helicobacter pylori: a new insight into pathogenicity. Chin Med J 2007;120:2138–2142 [CrossRef]
    [Google Scholar]
  45. Silva B, Nunes A, Vale FF, Rocha R, Gomes JP et al. The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion. Helicobacter 2017;22:e12390 [CrossRef]
    [Google Scholar]
  46. Ganguly M, Sarkar S, Ghosh P, Sarkar A, Alam J et al. Helicobacter pylori plasticity region genes are associated with the gastroduodenal diseases manifestation in India. Gut Pathog 2016;8:10 [CrossRef]
    [Google Scholar]
  47. Gu YF, Li Y, Song Y, Chang X, Qu YM et al. Biological function of hpsh4590 localized in the plasticity zone of Helicobacter pylori. Microb Pathog 2016;93:63–69 [CrossRef]
    [Google Scholar]
  48. Hoskisson PA, Hobbs G. Continuous culture-making a comeback?. Microbiology 2005;151:3153–3159 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000918
Loading
/content/journal/jmm/10.1099/jmm.0.000918
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error