1887

Abstract

The Roche Cobas 6800 CT/NG assay was compared to the Abbott m2000 Real Time CT/NG assay for detecting Chlamydia trachomatis and Neisseria gonorrhoeae in 714 specimens referred to the bacteriology laboratory at Geneva University Hospitals, between November 2017 and March 2018, and in nine external quality controls for molecular diagnostics (seven from QCMD Glasgow and two from UK NEQAS). For C. trachomatis , the sensitivity of C6800 compared to m2000 was 100 % (95 % confidence interval [CI], 97.5 to 100 %), the specificity was 99.1 % (95 % CI, 98.0 to 99.7 %). For N. gonorrhoeae , the sensitivity of the C6800 compared to m2000 was 100 % (95 % CI, 90.5 to 100 %), whereas the specificity was 99.7 % (95 % CI, 98.9 to 99.9 %). The C6800 CT/NG assay appears to perform with great accuracy the detection of C. trachomatis and N. gonorrhoeae .

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/content/journal/jmm/10.1099/jmm.0.000909
2019-01-03
2019-10-20
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References

  1. Newman L, Rowley J, vander Hoorn S, Wijesooriya NS, Unemo M et al. Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting. PLoS One 2015;10:e0143304 [CrossRef][PubMed]
    [Google Scholar]
  2. Smith DW, Tapsall JW, Lum G. Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network. Commun Dis Intell Q Rep 2005;29:358–365[PubMed]
    [Google Scholar]
  3. Marlowe EM, Hardy D, Krevolin M, Gohl P, Bertram A et al. High-throughput testing of urogenital and extragenital specimens for detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae with cobas CT/NG. Eur J Microbiol Immunol 2017;7:176–186 [CrossRef][PubMed]
    [Google Scholar]
  4. Centers for Disease Control and Prevention Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae-2014. MMWR Recomm Rep 2014;63:1-19 Recommendations and Reports / Vol. 63 / No. 2[PubMed]
    [Google Scholar]
  5. Sandkovsky Jon U, Sayles H, Bares S, Carr R, Tonozzi T et al. High rates of asymptomatic chlamydia and gonorrhea infection among HIV infected MSM. IDWeek 2015, San Diego, 7-11 October; Abstract 120. 2015;https://idsa.confex.com/idsa/2015/webprogram/Paper50444.html
  6. Kent CK, Chaw JK, Wong W, Liska S, Gibson S et al. Prevalence of rectal, urethral, and pharyngeal chlamydia and gonorrhea detected in 2 clinical settings among men who have sex with men: San Francisco, California, 2003. Clin Infect Dis 2005;41:67–74 [CrossRef][PubMed]
    [Google Scholar]
  7. Schachter J, Moncada J, Liska S, Shayevich C, Klausner JD. Nucleic acid amplification tests in the diagnosis of chlamydial and gonococcal infections of the oropharynx and rectum in men who have sex with men. Sex Transm Dis 2008;35:637–642 [CrossRef][PubMed]
    [Google Scholar]
  8. David Hardy PG, Lewinski M, Arcenas R, Seiverth B, Tanja SEM et al. Performance of the cobas CT/NG for use on the cobas 6800/8800 systems for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in oropharyngeal and anorectal swab specimens. Abstract 27th ECCMID 2017
    [Google Scholar]
  9. Greub G, Sahli R, Brouillet R, Jaton K. Ten years of R&D and full automation in molecular diagnosis. Future Microbiol 2016;11:403–425 [CrossRef][PubMed]
    [Google Scholar]
  10. Jaton K, Bille J, Greub G. A novel real-time PCR to detect Chlamydia trachomatis in first-void urine or genital swabs. J Med Microbiol 2006;55:1667–1674 [CrossRef][PubMed]
    [Google Scholar]
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