%0 Journal Article %A Uechi, Kohei %A Tada, Tatsuya %A Kuwahara-Arai, Kyoko %A Sekiguchi, Jun-ichiro %A Yanagisawa, Izumi %A Tome, Takaaki %A Nakasone, Isamu %A Maeda, Shiro %A Mya, San %A Zan, Khin Nyein %A Tin, Htay Htay %A Kirikae, Teruo %A Fujita, Jiro %T An improved carbapenem inactivation method, CIMTrisII, for carbapenemase production by Gram-negative pathogens %D 2019 %J Journal of Medical Microbiology, %V 68 %N 2 %P 124-131 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000888 %K Carbapenemase %K Enterobacteriaceae %K Carbapenem resistance %K Carbapenem inactivation method %K Acinetobacter species %K Pseudomonas species %I Microbiology Society, %X The modified carbapenem inactivation method (mCIM) is a simple phenotypic screening method for detecting carbapenemase production by Enterobacteriaceae and Pseudomonas aeruginosa . We recently developed another modified carbapenem inactivation method (CIMTris), in which carbapenemase is extracted from bacteria with Tris-HCl buffer, to detect carbapenemase production by Acinetobacter and Pseudomonas species. This study describes an improved carbapenem inactivation method, CIMTrisII, for detecting carbapenemase production by Gram-negative pathogens, including Enterobacteriaceae , Acinetobacter and Pseudomonas species. CIMTrisII was different from CIMTris in the concentration of Meropenem disks (5-µg MEM disks vs. 10-µg MEM disks), the inoculum volume of the bacteria (a 5-µl loopful vs. a 10 µl loopful) and the incubation time (1 vs. 2 h). CIMTrisII showed an overall sensitivity of 99.3 % and an overall specificity of 95.0 % for tested isolates. In comparison, CIMTris showed a sensitivity of 96.1 % and a specificity of 96.3 %, and mCIM showed a sensitivity of 67.1 % and a specificity of 100 %. CIMTrisII is thus deemed useful for detecting carbapenemase production by Gram-negative pathogens. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000888