1887

Journal of Medical Microbiology

Volume 68, Issue 2

An improved carbapenem inactivation method, CIMTrisII, for carbapenemase production by Gram-negative pathogens Free

Abstract

The modified carbapenem inactivation method (mCIM) is a simple phenotypic screening method for detecting carbapenemase production by Enterobacteriaceae and Pseudomonas aeruginosa . We recently developed another modified carbapenem inactivation method (CIMTris), in which carbapenemase is extracted from bacteria with Tris-HCl buffer, to detect carbapenemase production by Acinetobacter and Pseudomonas species. This study describes an improved carbapenem inactivation method, CIMTrisII, for detecting carbapenemase production by Gram-negative pathogens, including Enterobacteriaceae , Acinetobacter and Pseudomonas species. CIMTrisII was different from CIMTris in the concentration of Meropenem disks (5-µg MEM disks vs. 10-µg MEM disks), the inoculum volume of the bacteria (a 5-µl loopful vs. a 10 µl loopful) and the incubation time (1 vs. 2 h). CIMTrisII showed an overall sensitivity of 99.3 % and an overall specificity of 95.0 % for tested isolates. In comparison, CIMTris showed a sensitivity of 96.1 % and a specificity of 96.3 %, and mCIM showed a sensitivity of 67.1 % and a specificity of 100 %. CIMTrisII is thus deemed useful for detecting carbapenemase production by Gram-negative pathogens.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter species , Carbapenem inactivation method , Carbapenem resistance , Carbapenemase , Enterobacteriaceae and Pseudomonas species
© 2019 The Authors
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2018-12-10
2020-01-22
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An improved carbapenem inactivation method, CIMTrisII, for carbapenemase production by Gram-negative pathogens
J. Med. Microbiol. 68, 124 (2019); https://doi.org/10.1099/jmm.0.000888
/content/journal/jmm/10.1099/jmm.0.000888
/content/journal/jmm/10.1099/jmm.0.000888
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