1887

Abstract

In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commercial poultry flocks, and may be linked to zoonotic infections in humans. Here, we describe the genotypic and phenotypic characteristics of a multidrug-resistant APEC ST69 isolate (APECA2), recovered in 2016 from a diseased broiler at post-mortem examination in Germany. The isolate was resistant to several antibiotics of human and veterinary importance, including colistin. The mcr-1 gene was detected on a mobile genetic element located on an IncHI2/ST4 plasmid, which was characterized using long-read Nanopore and short-read Illumina sequencing of purified plasmid. Isolate APECA2 displayed resistance to chicken serum and harbours numerous virulence genes. This study highlights the public health importance of enhanced antimicrobial resistance surveillance and strict antimicrobial stewardship in human and veterinary healthcare.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000882
2018-11-26
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/68/1/111.html?itemId=/content/journal/jmm/10.1099/jmm.0.000882&mimeType=html&fmt=ahah

References

  1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016;16:161–168 [CrossRef][PubMed]
    [Google Scholar]
  2. Schwarz S, Johnson AP. Transferable resistance to colistin: a new but old threat. J Antimicrob Chemother 2016;71:2066–2070 [CrossRef][PubMed]
    [Google Scholar]
  3. Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill 2016;21:30280
    [Google Scholar]
  4. Yin W, Li H, Shen Y, Liu Z, Wang S et al. Novel plasmid-mediated colistin resistance gene mcr-3 in Escherichia coli. MBio 2017;8:e0054317 [CrossRef][PubMed]
    [Google Scholar]
  5. Borowiak M, Fischer J, Hammerl JA, Hendriksen RS, Szabo I et al. Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B. J Antimicrob Chemother 2017;72:3317–3324 [CrossRef][PubMed]
    [Google Scholar]
  6. Carattoli A, Villa L, Feudi C, Curcio L, Orsini S et al. Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016. Euro Surveill 2017;22:30589 [CrossRef][PubMed]
    [Google Scholar]
  7. Collingwood C, Kemmett K, Williams N, Wigley P. Is the concept of avian pathogenic Escherichia coli as a single pathotype fundamentally flawed?. Front Vet Sci 2014;1:5 [CrossRef][PubMed]
    [Google Scholar]
  8. Ewers C, Göttig S, Bülte M, Fiedler S, Tietgen M et al. Genome sequence of avian Escherichia coli strain IHIT25637, an extraintestinal pathogenic E. coli strain of ST131 encoding colistin resistance determinant MCR-1. Genome Announc 2016;4:e0086316 [CrossRef][PubMed]
    [Google Scholar]
  9. Antipov D, Hartwick N, Shen M, Raiko M, Lapidus A et al. plasmidSPAdes: assembling plasmids from whole genome sequencing data. Bioinformatics 2016;32:3380–3387 [CrossRef][PubMed]
    [Google Scholar]
  10. Antipov D, Korobeynikov A, McLean JS, Pevzner PA. hybridSPAdes: an algorithm for hybrid assembly of short and long reads. Bioinformatics 2016;32:1009–1015 [CrossRef][PubMed]
    [Google Scholar]
  11. Darling AE, Mau B, Perna NT. progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One 2010;5:e11147 [CrossRef][PubMed]
    [Google Scholar]
  12. Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H et al. Multilocus sequence typing of total-genome-sequenced bacteria. J Clin Microbiol 2012;50:1355–1361 [CrossRef][PubMed]
    [Google Scholar]
  13. Clermont O, Christenson JK, Denamur E, Gordon DM. The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environ Microbiol Rep 2013;5:58–65 [CrossRef][PubMed]
    [Google Scholar]
  14. Joensen KG, Scheutz F, Lund O, Hasman H, Kaas RS et al. Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli. J Clin Microbiol 2014;52:1501–1510 [CrossRef][PubMed]
    [Google Scholar]
  15. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother 2012;67:2640–2644 [CrossRef][PubMed]
    [Google Scholar]
  16. Veldman K, van Essen-Zandbergen A, Rapallini M, Wit B, Heymans R et al. Location of colistin resistance gene mcr-1 in Enterobacteriaceae from livestock and meat. J Antimicrob Chemother 2016;71:2340–2342 [CrossRef][PubMed]
    [Google Scholar]
  17. Poirel L, Kieffer N, Nordmann P. In Vitro study of ISApl1-mediated mobilization of the colistin resistance gene mcr-1. Antimicrob Agents Chemother 2017;61:e0012717 [CrossRef][PubMed]
    [Google Scholar]
  18. Snesrud E, Mcgann P, Chandler M. The birth and demise of the ISApl1-mcr-1-ISApl1 composite transposon: the vehicle for transferable colistin resistance. MBio 2018;9:e0238117 [CrossRef][PubMed]
    [Google Scholar]
  19. Kallonen T, Brodrick HJ, Harris SR, Corander J, Brown NM et al. Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131. Genome Res 2017;27:1437–1449 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000882
Loading
/content/journal/jmm/10.1099/jmm.0.000882
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error