Conventional culture method and qPCR using 16S rDNA for tissue bank: a comparison using a model of cardiac tissue contamination

References

1. Brubaker S, Lotherington K, Zhao J, Hamilton B, Rockl G et al. Tissue recovery practices and bioburden: a systematic review. Cell Tissue Bank 2016; 17:561–571 [View Article][PubMed]
   [Google Scholar]
2. Kraft L, Ribeiro VST, Wollmann LC, Suss PH, Tuon FF. Bioburden in transport solutions of human cardiovascular tissues: a comparative evaluation of direct inoculation and membrane filter technique. Cell Tissue Bank 2018; 19:447–454 [View Article][PubMed]
   [Google Scholar]
3. Germain M, Thibault L, Jacques A, Tremblay J, Bourgeois R. Heart valve allograft decontamination with antibiotics: impact of the temperature of incubation on efficacy. Cell Tissue Bank 2010; 11:197–204 [View Article][PubMed]
   [Google Scholar]
4. van Kats JP, van Tricht C, van Dijk A, van der Schans M, van den Bogaerdt A et al. Microbiological examination of donated human cardiac tissue in heart valve banking. Eur J Cardiothorac Surg 2010; 37:163–169 [View Article][PubMed]
   [Google Scholar]
5. ANVISA Boas Práticas em Tecidos humanos para uso terapêutico. Resolução RDC n55 2015
   [Google Scholar]
6. Heng WL, Seck T, Tay CP, Chua A, Song C et al. Homograft banking in Singapore: two years of cardiovascular tissue banking in Southeast Asia. Cell Tissue Bank 2013; 14:187–194 [View Article][PubMed]
   [Google Scholar]
7. Villalba R, Solis F, Fornés G, Jimenez A, Eisman M et al. In vitro susceptibility of high virulence microorganisms isolated in heart valve banking. Cell Tissue Bank 2012; 13:441–445 [View Article][PubMed]
   [Google Scholar]
8. Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF et al. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev 2006; 19:165–256 [View Article][PubMed]
   [Google Scholar]
9. Kraft L, Tuon FF, Ribeiro VST, Wollmann LC, Suss PH et al. Comparison of automated and conventional microbiological examination of donated human cardiac tissue in heart valve banking. Cell Tissue Bank 2018 [View Article][PubMed]
   [Google Scholar]
10. Fournier PE, Thuny F, Richet H, Lepidi H, Casalta JP et al. Comprehensive diagnostic strategy for blood culture-negative endocarditis: a prospective study of 819 new cases. Clin Infect Dis 2010; 51:131–140 [View Article][PubMed]
    [Google Scholar]
11. Miyazato A, Ohkusu K, Tabata M, Uwabe K, Kawamura T et al. Comparative molecular and microbiological diagnosis of 19 infective endocarditis cases in which causative microbes were identified by PCR-based DNA sequencing from the excised heart valves. J Infect Chemother 2012; 18:318–323 [View Article][PubMed]
    [Google Scholar]
12. Wimberly BT, Brodersen DE, Clemons WM, Morgan-Warren RJ, Carter AP et al. Structure of the 30S ribosomal subunit. Nature 2000; 407:327–339 [View Article][PubMed]
    [Google Scholar]
13. Peters RP, van Agtmael MA, Gierveld S, Danner SA, Groeneveld AB et al. Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit. J Clin Microbiol 2007; 45:3641–3646 [View Article][PubMed]
    [Google Scholar]
14. Marín M, Muñoz P, Sánchez M, del Rosal M, Alcalá L et al. Molecular diagnosis of infective endocarditis by real-time broad-range polymerase chain reaction (PCR) and sequencing directly from heart valve tissue. Medicine 2007; 86:195–202 [View Article][PubMed]
    [Google Scholar]
15. Millar BC, Jiru X, Moore JE, Earle JA. A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material. J Microbiol Methods 2000; 42:139–147 [View Article][PubMed]
    [Google Scholar]
16. Yang S, Lin S, Kelen GD, Quinn TC, Dick JD et al. Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens. J Clin Microbiol 2002; 40:3449–3454 [View Article][PubMed]
    [Google Scholar]
17. Varettas K. RT-PCR testing of allograft musculoskeletal tissue: is it time for culture-based methods to move over?. Pathology 2014; 46:640–643 [View Article][PubMed]
    [Google Scholar]
18. Cherkaoui A, Emonet S, Ceroni D, Candolfi B, Hibbs J et al. Development and validation of a modified broad-range 16S rDNA PCR for diagnostic purposes in clinical microbiology. J Microbiol Methods 2009; 79:227–231 [View Article][PubMed]
    [Google Scholar]
19. Insa R, Marín M, Martín A, Martín-Rabadán P, Alcalá L et al. Systematic use of universal 16S rRNA gene polymerase chain reaction (PCR) and sequencing for processing pleural effusions improves conventional culture techniques. Medicine 2012; 91:103–110 [View Article][PubMed]
    [Google Scholar]
20. Rampini SK, Bloemberg GV, Keller PM, Büchler AC, Dollenmaier G et al. Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections. Clin Infect Dis 2011; 53:1245–1251 [View Article][PubMed]
    [Google Scholar]
21. Rogers GB, Russell LE, Preston PG, Marsh P, Collins JE et al. Characterisation of bacteria in ascites–reporting the potential of culture-independent, molecular analysis. Eur J Clin Microbiol Infect Dis 2010; 29:533–541 [View Article][PubMed]
    [Google Scholar]
22. Vollmer T, Piper C, Horstkotte D, Körfer R, Kleesiek K et al. 23S rDNA real-time polymerase chain reaction of heart valves: a decisive tool in the diagnosis of infective endocarditis. Eur Heart J 2010; 31:1105–1113 [View Article][PubMed]
    [Google Scholar]
23. Schabereiter-Gurtner C, Nehr M, Apfalter P, Makristathis A, Rotter ML et al. Evaluation of a protocol for molecular broad-range diagnosis of culture-negative bacterial infections in clinical routine diagnosis. J Appl Microbiol 2008; 104:1228–1237 [View Article][PubMed]
    [Google Scholar]
24. Dalla-Costa LM, Morello LG, Conte D, Pereira LA, Palmeiro JK et al. Comparison of DNA extraction methods used to detect bacterial and yeast DNA from spiked whole blood by real-time PCR. J Microbiol Methods 2017; 140:61–66 [View Article][PubMed]
    [Google Scholar]
25. Reesink HW, Mohammadi T, Pietersz RN, Savelkoul PH. Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products. Clin Chem Lab Med 2008; 46:954–962 [View Article][PubMed]
    [Google Scholar]
26. Regan JF, Furtado MR, Brevnov MG, Jordan JA. A sample extraction method for faster, more sensitive PCR-based detection of pathogens in blood culture. J Mol Diagn 2012; 14:120–129 [View Article][PubMed]
    [Google Scholar]
27. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Kaczmarski EB et al. Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol 2000; 38:1747–1752[PubMed]
    [Google Scholar]
28. Dreier J, Störmer M, Kleesiek K. Real-time polymerase chain reaction in transfusion medicine: applications for detection of bacterial contamination in blood products. Transfus Med Rev 2007; 21:237–254 [View Article][PubMed]
    [Google Scholar]
29. Hoorfar J, Malorny B, Abdulmawjood A, Cook N, Wagner M et al. Practical considerations in design of internal amplification controls for diagnostic PCR assays. J Clin Microbiol 2004; 42:1863–1868 [View Article][PubMed]
    [Google Scholar]
30. Boussier R, Rogez S, François B, Denes E, Ploy MC et al. Two-step bacterial broad-range polymerase chain reaction analysis of heart valve tissue improves bacteriological diagnosis of infective endocarditis. Diagn Microbiol Infect Dis 2013; 75:240–244 [View Article][PubMed]
    [Google Scholar]
31. Harris KA, Yam T, Jalili S, Williams OM, Alshafi K et al. Service evaluation to establish the sensitivity, specificity and additional value of broad-range 16S rDNA PCR for the diagnosis of infective endocarditis from resected endocardial material in patients from eight UK and Ireland hospitals. Eur J Clin Microbiol Infect Dis 2014; 33:2061–2066 [View Article][PubMed]
    [Google Scholar]