1887

Abstract

is the cause of shigellosis or bacillary dysentery, the occurrence of which is estimated to be 165 million cases per year worldwide, resulting in 1.1 million deaths. Rapid and reliable assays for detecting and identifying in food, environmental and clinical samples are therefore necessary. species are traditionally identified by their O antigens. This study developed a DNA microarray targeting O-serotype-specific genes to detect all 34 distinct O-antigen forms of , including types 1–18, types 1–13, types 1–5 and 6, and . A total of 282 strains were used to test the specificity of the microarray, including 186 and representative strains, 86 clinical isolates and ten strains of other bacterial species that are commonly isolated from food or clinical stool specimens. The oligonucleotide probes were printed on the microarray in concentrations from 1 to 100 μM, and 10 μM proved to be the optimal probe concentration. The detection sensitivity for each serotype was 50 ng genomic DNA or 1 c.f.u. in 25 g milk powder sample following a 6 h enrichment in broth. The microarray is specific, sensitive and reproducible, and, to our knowledge, is the first report of a microarray for serotyping all O-antigen forms of .

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2009-01-01
2019-10-23
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References

  1. Ahmed, F., Clemens, J. D., Rao, M. R., Ansaruzzaman, M. & Haque, E. ( 1997; ). Epidemiology of shigellosis among children exposed to cases of Shigelladysentery: a multivariate assessment. Am J Trop Med Hyg 56 , 258 –264.
    [Google Scholar]
  2. Bennish, M. L. ( 1991; ). Potentially lethal complications of shigellosis. Rev Infect Dis 13 (Suppl. 4), S319–S324.[CrossRef]
    [Google Scholar]
  3. Beutin, L., Tao, J., Feng, L., Krause, G., Zimmermann, S., Gleier, K., Xia, Q. & Wang, L. ( 2005; ). Sequence analysis of the Escherichia coli O15 antigen gene cluster and development of a PCR assay for rapid detection of intestinal and extraintestinal pathogenic E. coli O15 strains. J Clin Microbiol 43, 703–710.[CrossRef]
    [Google Scholar]
  4. Bopp, C. A., Brenner, F. W., Fields, P. I., Wells, J. G. & Strockbine, N. A. ( 2003; ). Escherichia, Shigella, and Salmonella. In Manual of Clinical Microbiology, 8th edn, pp. 654–671. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  5. Casalino, M., Latella, M. C., Prosseda, G. & Colonna, B. ( 2003; ). CadC is the preferential target of a convergent evolution driving enteroinvasive Escherichia coli toward a lysine decarboxylase-defective phenotype. Infect Immun 71, 5472–5479.[CrossRef]
    [Google Scholar]
  6. Cheah, K. Y., Beger, D. W. & Manning, P. A. ( 1991; ). Molecular cloning and genetic analysis of the rfb region from Shigella flexneri type 6 in Escherichia coli K-12. FEMS Microbiol Lett 83, 213–218.
    [Google Scholar]
  7. DebRoy, C., Fratamico, P. M., Roberts, E., Davis, M. A. & Liu, Y. ( 2005; ). Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups. Appl Environ Microbiol 71, 4919–4924.[CrossRef]
    [Google Scholar]
  8. DuPont, H. L., Levine, M. M., Hornick, R. B. & Formal, S. B. ( 1989; ). Inoculum size in shigellosis and implications for expected mode of transmission. J Infect Dis 159, 1126–1128.[CrossRef]
    [Google Scholar]
  9. Feng, L., Tao, J., Guo, H., Xu, J., Li, Y., Rezwan, F., Reeves, P. & Wang, L. ( 2004a; ). Structure of the Shigella dysenteriae 7 O antigen gene cluster and identification of its antigen specific genes. Microb Pathog 36, 109–115.[CrossRef]
    [Google Scholar]
  10. Feng, L., Wang, W., Tao, J., Guo, H., Krause, G., Beutin, L. & Wang, L. ( 2004b; ). Identification of Escherichia coli O114 O-antigen gene cluster and development of an O114 serogroup-specific PCR assay. J Clin Microbiol 42, 3799–3804.[CrossRef]
    [Google Scholar]
  11. Fratamico, P. M., Briggs, C. E., Needle, D., Chen, C. Y. & DebRoy, C. ( 2003; ). Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes. J Clin Microbiol 41, 3379–3383.[CrossRef]
    [Google Scholar]
  12. Johnson, J. R. ( 2000; ). Shigella and Escherichia coli at the crossroads: machiavellian masqueraders or taxonomic treachery? J Med Microbiol 49, 583–585.
    [Google Scholar]
  13. Koterski, J. F., Nahvi, M., Venkatesan, M. M. & Haimovich, B. ( 2005; ). Virulent Shigella flexneri causes damage to mitochondria and triggers necrosis in infected human monocyte-derived macrophages. Infect Immun 73, 504–513.[CrossRef]
    [Google Scholar]
  14. Kotloff, K. L., Winickoff, J. P., Ivanoff, B., Clemens, J. D., Swerdlow, D. L., Sansonetti, P. J., Adak, G. K. & Levine, M. M. ( 1999; ). Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull World Health Organ 77, 651–666.
    [Google Scholar]
  15. Lai, V., Wang, L. & Reeves, P. R. ( 1998; ). Escherichia coli clone Sonnei (Shigella sonnei) had a chromosomal O-antigen gene cluster prior to gaining its current plasmid-borne O-antigen genes. J Bacteriol 180, 2983–2986.
    [Google Scholar]
  16. Lan, R. & Reeves, P. R. ( 2002; ). Escherichia coli in disguise: molecular origins of Shigella. Microbes Infect 4, 1125–1132.[CrossRef]
    [Google Scholar]
  17. Li, Y., Liu, D., Cao, B., Han, W., Liu, Y., Liu, F., Guo, X., Bastin, D. A., Feng, L. & Wang, L. ( 2006; ). Development of a serotype-specific DNA microarray for identification of some Shigella and pathogenic Escherichia coli strains. J Clin Microbiol 44, 4376–4383.[CrossRef]
    [Google Scholar]
  18. Liu, B., Knirel, Y. A., Feng, L., Perepelov, A. V., Senchenkova, S. N., Wang, Q., Reeves, P. & Wang, L. ( 2008; ). Structure and genetics of Shigella O antigens. FEMS Microbiol Rev 32, 627–653.[CrossRef]
    [Google Scholar]
  19. Maurelli, A. T., Fernandez, R. E., Bloch, C. A., Rode, C. K. & Fasano, A. ( 1998; ). “Black holes” and bacterial pathogenicity: a large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli. Proc Natl Acad Sci U S A 95, 3943–3948.[CrossRef]
    [Google Scholar]
  20. NIAID ( 2003; ). NIAID Biodefense Research Agenda for Category B and C Priority Pathogens. NIH Publication No. 03-5315.
  21. Panicker, G., Call, D. R., Krug, M. J. & Bej, A. K. ( 2004; ). Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays. Appl Environ Microbiol 70, 7436–7444.[CrossRef]
    [Google Scholar]
  22. Rouillard, J. M., Zuker, M. & Gulari, E. ( 2003; ). OligoArray 2.0: design of oligonucleotide probes for DNA microarrays using a thermodynamic approach. Nucleic Acids Res 31, 3057–3062.[CrossRef]
    [Google Scholar]
  23. Schena, M. ( 2003; ). Microarray surfaces. In Microarray Analysis, pp. 95–120. New York: Wiley.
  24. Shiga, K. ( 1906; ). Observations on the epidemiology of dysentery in Japan. Philipp J Sci 1, 485
    [Google Scholar]
  25. Simmons, D. A. R. & Romanowska, E. ( 1987; ). Structure and biology of Shigella flexneri O antigens. J Med Microbiol 23, 289–302.[CrossRef]
    [Google Scholar]
  26. Tao, J., Feng, L., Guo, H., Li, Y. & Wang, L. ( 2004; ). The O-antigen gene cluster of Shigella boydii O11 and functional identification of its wzy gene. FEMS Microbiol Lett 234, 125–132.[CrossRef]
    [Google Scholar]
  27. Tao, J., Wang, L., Liu, D., Li, Y., Bastin, D. A., Geng, Y. & Feng, L. ( 2005; ). Molecular analysis of Shigella boydii O1 O-antigen gene cluster and its PCR typing. Can J Microbiol 51, 387–392.[CrossRef]
    [Google Scholar]
  28. Venkatesan, M. M., Buysse, J. M. & Kopecko, D. J. ( 1989; ). Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shigella spp. and enteroinvasive Escherichia coli. J Clin Microbiol 27, 2687–2691.
    [Google Scholar]
  29. Wang, L., Liu, B., Kong, Q., Steinruck, H., Krause, G., Beutin, L. & Feng, L. ( 2005; ). Molecular markers for detection of pathogenic Escherichia coli strains belonging to serogroups O 138 and O 139. Vet Microbiol 111, 181–190.[CrossRef]
    [Google Scholar]
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