1887

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Volume 67, Issue 9

Pasteurella multocida inactivated with ferric chloride and adjuvanted with bacterial DNA is a potent and efficacious vaccine in Balb/c mice Free

Abstract

Purpose. Pasteurella multocida (P. multocida) is a principal pathogen of domestic animals and an opportunistic pathogen of humans. It is the causative agent of pneumonia and haemorrhagic septicaemia in cattle, sheep and goats, fowl cholera in chickens and progressive atrophic rhinitis in swine. In this study, we investigated the humoral and cellular immune responses and protective immunity conferred by an iron-inactivated vaccine with bacterial DNA (IIV+bDNA) as an adjuvant in mice.

Methodology. P. multocida was grown in BHI broth, inactivated with formalin and FeCl3 and adjuvanted with alum and bDNA. Mice were immunized with two whole-cell inactivated vaccine doses 2 weeks apart. The animals were challenged 4 weeks after booster immunization. Immunogens (vaccines and bDNA) posed no safety problems when mice were injected subcutaneously (s/c) with these preparations. The serum antibody titres were tested by ELISA. At 28 days post immunization, cell-mediated immunity responses were determined. The responses were measured by assay of IL-6 and IL-12 in lymphocyte spleen culture supernatants.

Results. ELISA results showed that the levels of antibodies in iron inactivated with bDNA adjuvant groups were higher than in the formalin inactivated with alum adjuvant vaccine group. The protection rate of IIV+bDNA adjuvant vaccine was superior to that of the other vaccines and it protected 100 % of the challenge group mice. Following immunization, bDNA promoted increased production of interleukins compared to the control groups.

Conclusion. These studies indicate that bDNA is effective as an immune adjuvant, and along with stimulatory bDNA represent promising new humoral and cellular immune enhancers for vaccination applications. In addition, this vaccine is able to provide long-term protection against infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): adjuvant , bacterial DNA , ferric chloride , Pasteurella multocida , spleen culture and vaccine
© 2018 The Authors
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2018-07-17
2024-04-18
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References

  1. Kumar A, Mohanty NN, Chacko N, Yogisharadhya R, Shivachandra SB. Structural features of a highly conserved Omp16 protein of Pasteurella multocida strains and comparison with related peptidoglycan-associated lipoproteins (PAL). Indian J Microbiol 2015; 55:50–56 [View Article]
     [Google Scholar]
  2. Daneshlari S, Tahamtan Y, Kargar M. Molecular analysis of virulence factors of Pasteurella multocida from sheep and goats. Online J Vet Res 2013; 17:675–684
     [Google Scholar]
  3. Shirzad-Aski H, Tabatabaei M. Molecular characterization of Pasteurella multocida isolates obtained from poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis. Mol Biol Res Commun 2016; 5:123–132[PubMed]
     [Google Scholar]
  4. Silveira M, Vargas S, Mendonça M, Cunha C, Hartwig D et al. Xanthan gum enhances humoral immune response elicited by a DNA vaccine against leptospirosis in mice. BMC Proc 2014; 8:P153 [View Article]
     [Google Scholar]
  5. Wilson BA, Ho M. Pasteurella multocida: from zoonosis to cellular microbiology. Clin Microbiol Rev 2013; 26:631–655 [View Article][PubMed]
     [Google Scholar]
  6. Ahmad AM. Efforts towards the development of recombinant vaccines against Pasteurella multocida. Sci World J 2014; 9:1–7
     [Google Scholar]
  7. Bacelo KL, Hartwig DD, Seixas FK, Schuch R, Moreira A et al. Xanthan gum as an adjuvant in a subunit vaccine preparation against leptospirosis. Biomed Res Int 2014; 2014:1–10 [View Article]
     [Google Scholar]
  8. Ashry El SH, Ahmad TA. The use of propolis as vaccine's adjuvant. Vaccine 2012; 31:31–39 [View Article][PubMed]
     [Google Scholar]
  9. Mapletoft JW, Oumouna M, Kovacs-Nolan J, Latimer L, Mutwiri G et al. Intranasal immunization of mice with a formalin-inactivated bovine respiratory syncytial virus vaccine co-formulated with CpG oligodeoxynucleotides and polyphosphazenes results in enhanced protection. J Gen Virol 2008; 89:250–260 [View Article][PubMed]
     [Google Scholar]
  10. Weiner GJ, Liu HM, Wooldridge JE, Dahle CE, Krieg AM. Immunostimulatory oligodeoxynucleotides containing the CpG motif are effective as immune adjuvants in tumor antigen immunization. Proc Natl Acad Sci USA 1997; 94:10833–10837 [View Article][PubMed]
     [Google Scholar]
  11. Trevani AS, Chorny A, Salamone G, Vermeulen M, Gamberale R et al. Bacterial DNA activates human neutrophils by a CpG-independent pathway. Eur J Immunol 2003; 33:3164–3174 [View Article][PubMed]
     [Google Scholar]
  12. Kumar D, Singh A. Salmonella typhimurium grown in iron-rich media, inactivated with ferric chloride and adjuvanted with homologous bacterial DNA is potent and efficacious vaccine in mice. Vaccine 2005; 23:5590–5598 [View Article][PubMed]
     [Google Scholar]
  13. Herath C, Kumar P, Singh M, Kumar D, Ramakrishnan S et al. Experimental iron-inactivated Pasteurella multocida A: 1 vaccine adjuvanted with bacterial DNA is safe and protects chickens from fowl cholera. Vaccine 2010; 28:2284–2289 [View Article][PubMed]
     [Google Scholar]
  14. Randhawa MA. Calculation of LD50 values from the method of Miller and Tainter, 1944. J Ayub Med Coll Abbottabad 2009; 21:184–185[PubMed]
     [Google Scholar]
  15. Cheng HR, Jiang N. Extremely rapid extraction of DNA from bacteria and yeasts. Biotechnol Lett 2006; 28:55–59 [View Article][PubMed]
     [Google Scholar]
  16. Singh S, Singh VP, Cheema PS, Sandey M, Ranjan R et al. Immune response to dna vaccine expressing transferrin binding protein a gene of Pasteurella multocida. Braz J Microbiol 2011; 42:750–760 [View Article][PubMed]
     [Google Scholar]
  17. Sahragard I, Tahamtan Y, Valadan M, Hyati M, Moazeni F et al. Development of rapid PCR method for simultaneous identification of species, specific capsular type, and toxigenicity of Pasteurella sp. isolates. Comp Clin Path 2012; 21:1333–1336 [View Article]
     [Google Scholar]
  18. Gong Q, Qin CL, Niu MF, Cheng M, Sun XF et al. Immune efficacy of OmpH and OmpA DNA vaccines against avian Pasteurella multocida. Iran J Vet Res 2013; 14:197–202
     [Google Scholar]
  19. Nies DH. Microbial heavy-metal resistance. Appl Microbiol Biotechnol 1999; 51:730–750 [View Article][PubMed]
     [Google Scholar]
  20. Radhi SN. Optimization of heavy metals chlorides resistance by Staphylococcus aureus and its ability to remove them. Iraqi J Sci 2012; 53:778–785
     [Google Scholar]
  21. Qureshi S, Saxena HM. Estimation of titers of antibody against Pasteurella multocida in cattle vaccinated with haemorrhagic septicemia alum precipitated vaccine. Vet World 2014; 7:224–228 [View Article]
     [Google Scholar]
  22. Okay S, Özcengiz E, Gürsel I, Özcengiz G. Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice. Res Vet Sci 2012; 93:1261–1265 [View Article][PubMed]
     [Google Scholar]
  23. Bode C, Zhao G, Steinhagen F, Kinjo T, Klinman DM. CpG DNA as a vaccine adjuvant. Expert Rev Vaccines 2011; 10:499–511 [View Article][PubMed]
     [Google Scholar]
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Pasteurella multocida inactivated with ferric chloride and adjuvanted with bacterial DNA is a potent and efficacious vaccine in Balb/c mice
J. Med. Microbiol. 67, 1383 (2018); https://doi.org/10.1099/jmm.0.000794
/content/journal/jmm/10.1099/jmm.0.000794
/content/journal/jmm/10.1099/jmm.0.000794
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