1887

Abstract

has emerged as a highly antimicrobial-resistant healthcare-associated pathogen, in particular for patients admitted to neonatal intensive care. The objective of this study was to study the nature of SCC types among MDR-SH strains isolated from paediatric patients.

strains (=60) were isolated from paediatric patients. Antibiotic resistance patterns were established using the disk agar diffusion and micro-broth dilution methods. SCC typing was performed using whole-genome sequencing (WGS) and an additional PCR analysis.

All isolates demonstrated multidrug resistance. Using WGS, various novel types and combinations of SCC types were found, including a new composite island [SCCmec type V (Vd)+SCC] comprising 30 % of the strains. SCC type V was identified in 23 % of the isolates. A combination of the gene enclosed by two copies and absence of the and genes was identified in 11 strains. In total, regulatory genes were absent in all SH isolates used in this study.

A high diversity of SCC elements with the prevalence of a new composite island was determined among MRSH strains. The structure of the composite island represented by MDR-SH strains in this study, in combination with the presence of a restriction-modification system type III, is described for the first time in this study. The presence of an 8 bp direct repeat (DR) and the sequences flanking the DR may support the integration of the gene complex as a composite transposon (IS-IS) independently from recombinase genes.

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2018-07-01
2020-01-29
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