Antifungal susceptibility and virulence of species complex: an overview of their pathogenic potential Free

Abstract

Antifungal resistance and several putative virulence factors have been associated with the pathogenicity of the species complex. The objective of this study was to evaluate the antifungal susceptibility, the production of virulence factors and the pathogenicity of the complex.

Overall, 49 isolates of , 19 and nine were used. The planktonic and biofilm susceptibility to fluconazole, itraconazole, voriconazole, amphotericin B and caspofungin was assessed using a broth microdilution assay. Finally, the production of biofilm and hydrolytic enzymes and the fungal pathogenicity against were investigated.

Overall, one was resistant to caspofungin and susceptible-dose-dependent to itraconazole, the other two were susceptible-dose-dependent to fluconazole and itraconazole, and one was susceptible-dose-dependent to azoles. A total of 67.5 % of the isolates were biofilm producers. Amphotericin B and caspofungin caused the greatest reduction in the metabolic activity and biomass of mature biofilms. Phospholipase and protease production was observed in 55.1 % of , 42.1 % of and 33.3 % of isolates. Moreover, 57.9 % of and 20.4 % of isolates were β-haemolytic, and all were α-haemolytic. Finally, the complex caused high mortality of after 96 h of exposure.

These results reinforce the heterogeneity of these cryptic species for their antifungal susceptibility, virulence and pathogenic potential, emphasizing the relevance of monitoring these emerging pathogens.

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2018-07-01
2024-03-28
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