1887

Abstract

We investigated the reliability of nalidixic acid (NA) susceptibility as a marker of ciprofloxacin susceptibility in Salmonella, analysing 302 stool isolates. NC53 of the MicroScan system was used for NA susceptibility tests and the E-test was used for ciprofloxacin susceptibility tests. Among the isolates, 178 (58.9 %) were serogroup B, 74 (24.5 %) were serogroup D, 27 (8.9 %) were serogroup C and 23 (7.6 %) were from other minor serogroups. Globally, susceptibility to NA correctly predicted the susceptibility of Salmonella to ciprofloxacin, with a sensitivity of 81.5 %, a specificity of 97.6 %, and positive and negative predictive values of 88 and 96 %, respectively. However, there were differences among the serogroups in terms of sensitivity (P<0.001) and positive predictive values (P=0.013). NA is a reliable marker for serogroup D, but not for serogroups B or C. According to these findings, NA susceptibility measured with the MicroScan system can be used as a marker of ciprofloxacin resistance in some serogroups in our setting.

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2018-05-16
2019-10-13
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References

  1. Crum-Cianflone NF. Salmonellosis and the gastrointestinal tract: more than just peanut butter. Curr Gastroenterol Rep 2008;10:424–431 [CrossRef][PubMed]
    [Google Scholar]
  2. Sánchez-Capilla AD, Sorlózano-Puerto A, Rodríguez-Granger J, Martínez-Brocal A, Navarro-Marí JM et al. Infectious etiology of diarrheas studied in a third-level hospital during a five-year period. Rev Esp Enferm Dig 2015;107:89–97[PubMed]
    [Google Scholar]
  3. Hohmann EL. Nontyphoidal salmonellosis. Clin Infect Dis 2001;32:263–269 [CrossRef][PubMed]
    [Google Scholar]
  4. Cummings KJ, Warnick LD, Gröhn YT, Hoelzer K, Root TP et al. Clinical features of human salmonellosis caused by bovine-associated subtypes in New York. Foodborne Pathog Dis 2012;9:796–802 [CrossRef][PubMed]
    [Google Scholar]
  5. Jiménez-Guerra G, Foronda García-Hidalgo C, Moreno-Torres IS, Gutiérrez Fernández J, Navarro Marí JM. Sensibilidad antibiótica de Campylobacter spp. y de Salmonella enterica en pacientes con enteritis. Rev Soc Andaluza Microbiol Parasitol Clín 2017;1:10
    [Google Scholar]
  6. Gómez-Camarasa C, Gutiérrez-Fernández J, Rodríguez-Granger JM, Sampedro-Martínez A, Sorlózano-Puerto A et al. Evaluation of the rapid RIDAQUICK Campylobacter® test in a general hospital. Diagn Microbiol Infect Dis 2014;78:101–104 [CrossRef][PubMed]
    [Google Scholar]
  7. CLSI Performance Standards for Antimicrobial Susceptibility Testing, 27th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2017
    [Google Scholar]
  8. Mehta CR, Patel NR. Algorithm 643: FEXACT: a FORTRAN subroutine for Fisher's exact test on unordered r×c contingency tables. ACM Trans Math Softw 1986;12:154–161 [CrossRef]
    [Google Scholar]
  9. Angelo KM, Reynolds J, Karp BE, Hoekstra RM, Scheel CM et al. Antimicrobial resistance among nontyphoidal Salmonella isolated from blood in the United States, 2003–2013. J Infect Dis 2016;214:1565–1570 [CrossRef][PubMed]
    [Google Scholar]
  10. Ryan MP, Dillon C, Adley CC. Nalidixic acid-resistant strains of Salmonella showing decreased susceptibility to fluoroquinolones in the midwestern region of the Republic of Ireland due to mutations in the gyrA gene. J Clin Microbiol 2011;49:2077–2079 [CrossRef][PubMed]
    [Google Scholar]
  11. Crump JA, Barrett TJ, Nelson JT, Angulo FJ. Reevaluating fluoroquinolone breakpoints for Salmonella enterica serotype Typhi and for non-Typhi Salmonellae. Clin Infect Dis 2003;37:75–81 [CrossRef][PubMed]
    [Google Scholar]
  12. Hakanen A, Kotilainen P, Jalava J, Siitonen A, Huovinen P. Detection of decreased fluoroquinolone susceptibility in Salmonellas and validation of nalidixic acid screening test. J Clin Microbiol 1999;37:3572–3577[PubMed]
    [Google Scholar]
  13. Ray P, Sharma J, Marak RS, Garg RK. Predictive efficacy of nalidixic acid resistance as a marker of fluoroquinolone resistance in Salmonella enterica var Typhi. Indian J Med Res 2006;124:105–108[PubMed]
    [Google Scholar]
  14. Kapil A, Renuka, Das B. Nalidixic acid susceptibility test to screen ciprofloxacin resistance in Salmonella typhi. Indian J Med Res 2002;115:49–54[PubMed]
    [Google Scholar]
  15. Hakanen AJ, Lindgren M, Huovinen P, Jalava J, Siitonen A et al. New quinolone resistance phenomenon in Salmonella enterica: nalidixic acid-susceptible isolates with reduced fluoroquinolone susceptibility. J Clin Microbiol 2005;43:5775–5778 [CrossRef][PubMed]
    [Google Scholar]
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