1887

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Volume 67, Issue 5

High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Open Access

Abstract

Adherence capacity is one of the principal virulence factors of , and adhesion virulence factors are controlled by small RNAs (sRNAs) at the post-transcriptional level in various bacteria. Here, we aimed to identify and decipher putative adhesion-related sRNAs in clinical strains of

RNA deep-sequencing was performed to identify potential sRNAs under different adhesion conditions. The expression of sRNAs was analysed by quantitative real-time PCR (qRT-PCR), and bioinformatic methods were used to predict the functional characteristics of sRNAs.

A total of 736 differentially expressed candidate sRNAs were predicted, and these included 352 sRNAs located on the antisense to mRNA (AM) and 384 sRNAs in intergenic regions (IGRs). The top 7 differentially expressed sRNAs were successfully validated by qRT-PCR in UA159, and 2 of these were further confirmed in 100 clinical isolates. Moreover, the sequences of two sRNAs were conserved in other species, indicating a conserved role in such closely related species. A good correlation between the expression of sRNAs and the adhesion of 100 clinical strains was observed, which, combined with GO and KEGG, provides a perspective for the comprehension of sRNA function annotation.

This study revealed a multitude of novel putative adhesion-related sRNAs in and contributed to a better understanding of information concerning the transcriptional regulation of adhesion in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): adherence , clinical strains , dental caries , small RNAs and Streptococcus mutans
© 2018 The Authors
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2018-05-01
2024-04-25
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High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans
J. Med. Microbiol. 67, 641 (2018); https://doi.org/10.1099/jmm.0.000718
/content/journal/jmm/10.1099/jmm.0.000718
/content/journal/jmm/10.1099/jmm.0.000718
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