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Abstract

Purpose. In traditional Korean medicine, Artemisia apiacea H. (ART) and Scutellaria baicalensis G. (SCU) are combined for the treatment of malaria and other malaria-like diseases. Because SCU is well-known as an antibacterial agent, the antimicrobial effect of a mixture of ART and SCU was investigated.

Methodology. Plant samples were purchased from Kyungdong mart and extracted with 70 % ethanol. The in vitro antimicrobial activity of ART and SCU against pathogenic fungi (Aspergillus niger, Aspergillus oryzae, Candida albicans, Candida tropicalis and Candida glabrata), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) was evaluated using a broth microdilution assay, modified-disc diffusion and agar dilution methods with further CH2Cl2-fractionated ART, SCU and a mixture of ART/SCU (at a ratio of 3 : 5) (THAN-1).

Results. ART and SCU were effective against A. niger, C. albicans, B. subtilis and S. aureus. The range of minimum inhibitory concentration (MIC) values was 0.03125 to 4 mg ml in the ART and SCU treatments. ART exhibited stronger activity than SCU. Interestingly, a 3 : 5 ratio mixture of ART and SCU (THAN-1) showed stronger antimicrobial activity than ART or SCU used individually.

Conclusion. A treatment using a mixture of herbs such as THAN-1 would be useful in the suppression of the growth of pathogenic bacterial and fungal strains.

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2018-03-05
2019-10-23
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References

  1. Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. J Adv Pharm Technol Res 2011; 2: 104 [CrossRef] [PubMed]
    [Google Scholar]
  2. Iwu MW, Duncan AR, Okunji CO. New antimicrobials of plant origin. Perspectives on New Crops and New Uses Alexandria, VA: ASHS Press; 1999; pp. 457– 462
    [Google Scholar]
  3. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12: 564– 582 [PubMed]
    [Google Scholar]
  4. Dalene BS. The benefits of liquid herbal extracts. http://natural-fertility-info.com/benefits-of-liquid-herbal-extracts.html [Accessed 24 Mar 2017]
  5. Lee SJ, Kim HM, Lee JM, Park HS, Lee S. Artemisterol, a new steryl ester from the whole plant of Artemisia apiacea. J Asian Nat Prod Res 2008; 10: 281– 283 [CrossRef] [PubMed]
    [Google Scholar]
  6. Pan SY, Zhou SF, Gao SH, Yu ZL, Zhang SF et al. New perspectives on how to discover drugs from herbal medicines: CAM's outstanding contribution to modern therapeutics. Evid Based Complement Alternat Med 2013; 2013: 1– 25 [CrossRef] [PubMed]
    [Google Scholar]
  7. Ryu JC, Park SM, Hwangbo M, Byun SH, Ku SK et al. Methanol extract of Artemisia apiacea hance attenuates the expression of inflammatory mediators via NF-κB inactivation. Evid Based Complement Alternat Med 2013; 2013: 1– 12
    [Google Scholar]
  8. Kim KS, Shim SH, Jang JM, Cheong JH, Kim BK. A study on hair-growth activity of Artemisia apiacea Hance. Yakhakhoe-chi 1999; 43: 798– 801
    [Google Scholar]
  9. Hsu E. The history of qing hao in the Chinese materia medica. Trans R Soc Trop Med Hyg 2006; 100: 505– 508 [CrossRef] [PubMed]
    [Google Scholar]
  10. Tan RX, Zheng WF, Tang HQ. Biologically active substances from the genus Artemisia. Planta Med 1998; 64: 295– 302 [CrossRef] [PubMed]
    [Google Scholar]
  11. Tian XY, Cheung LM, Leung K, Qi C, Deng B et al. The effects of Scutellaria baicalensis extract on embryonic development in mice. Birth Defects Res B Dev Reprod Toxicol 2009; 86: 79– 84 [CrossRef] [PubMed]
    [Google Scholar]
  12. Britto A, Sebastian SR, Sujin RM. Antibacterial activity of selected species of Lamiaceae against human pathogens. Indian J Nat Prod Resour 2012; 3: 334– 342
    [Google Scholar]
  13. Park YH, Seo WT. Flavonoid Production from a Cell Suspension Culture of Scutellaria Baicalensis G. Biochemical Engineering for 2001 Tokyo: Springer; 1992; pp. 250– 253
    [Google Scholar]
  14. Heo HJ, Kim DO, Choi SJ, Shin DH, Lee CY. Potent Inhibitory effect of flavonoids in Scutellaria baicalensis on amyloid beta protein-induced neurotoxicity. J Agric Food Chem 2004; 52: 4128– 4132 [CrossRef] [PubMed]
    [Google Scholar]
  15. Li-Weber M. New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat Rev 2009; 35: 57– 68 [CrossRef] [PubMed]
    [Google Scholar]
  16. Li HB, Chen F. Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography. J Chromatogr A 2005; 1074: 107– 110 [PubMed] [Crossref]
    [Google Scholar]
  17. Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother 2001; 48: 5– 16 [CrossRef] [PubMed]
    [Google Scholar]
  18. Serrano MC, Ramírez M, Morilla D, Valverde A, Chávez M et al. A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp. J Antimicrob Chemother 2004; 53: 739– 742 [CrossRef] [PubMed]
    [Google Scholar]
  19. Hanson CW, Martin WJ. Modified agar dilution method for rapid antibiotic susceptibility testing of anaerobic bacteria. Antimicrob Agents Chemother 1978; 13: 383– 388 [CrossRef] [PubMed]
    [Google Scholar]
  20. Avula B, Wang YH, Smillie TJ, Mabusela W, Vincent L et al. Comparison of LC–UV, LC–ELSD and LC–MS methods for the determination of sesquiterpenoids in various species of Artemisia. Chromatographia 2009; 70: 797– 803 [CrossRef]
    [Google Scholar]
  21. Moon YH, Min BS, Bae KH, Lee YH. Antibacterial constituents from Scutellariae Radix against Streptococcus mutans OMZ176. Korean J Pharmacogn 1997; 28: 99– 103
    [Google Scholar]
  22. Zhao Q, Chen XY, Martin C. Scutellaria baicalensis, the golden herb from the garden of Chinese medicinal plants. Sci Bull 2016; 61: 1391– 1398 [CrossRef] [PubMed]
    [Google Scholar]
  23. Johann S, Oliveira VL, Pizzolatti MG, Schripsema J, Braz-Filho R et al. Antimicrobial activity of wax and hexane extracts from Citrus spp. peels. Mem Inst Oswaldo Cruz 2007; 102: 681– 685 [CrossRef] [PubMed]
    [Google Scholar]
  24. Seo KS, Jeong HJ, Yun KW. Antimicrobial activity and chemical components of two plants, Artemisia capillaris and Artemisia iwayomogi, used as Korean herbal Injin. J Ecol Environ 2010; 33: 141– 147 [CrossRef]
    [Google Scholar]
  25. Gasiorowski K, Lamer-Zarawska E, Leszek J, Parvathaneni K, Yendluri BB et al. Flavones from root of Scutellaria baicalensis Georgi: drugs of the future in neurodegeneration?. CNS Neurol Disord Drug Targets 2011; 10: 184– 191 [CrossRef] [PubMed]
    [Google Scholar]
  26. Cai W, Fu Y, Zhang W, Chen X, Zhao J et al. Synergistic effects of baicalein with cefotaxime against Klebsiella pneumoniae through inhibiting CTX-M-1 gene expression. BMC Microbiol 2016; 16: 181 [CrossRef] [PubMed]
    [Google Scholar]
  27. Tan CX, Schrader KK, Khan IA, Rimando AM. Activities of wogonin analogs and other flavones against Flavobacterium columnare. Chem Biodivers 2015; 12: 259– 272 [CrossRef] [PubMed]
    [Google Scholar]
  28. Suresh Babu K, Hari Babu T, Srinivas PV, Sastry BS, Hara Kishore K et al. Synthesis and in vitro study of novel 7-O-acyl derivatives of Oroxylin A as antibacterial agents. Bioorg Med Chem Lett 2005; 15: 3953– 3956 [CrossRef] [PubMed]
    [Google Scholar]
  29. Lepilleur CA. "Cationic cassia derivatives and applications therefor". U.S. Patent No. 7,704,934 2010
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