1887

Abstract

Hepatitis E virus (HEV) is an RNA virus that is an important cause of both acute and chronic hepatitis worldwide. To date, there are eight HEV genotypes that can infect mammals. HEV-1 and HEV-2 infect exclusively humans, while HEV-3 and HEV-4 infect humans and various animals, mainly pigs and deer. Additionally, two new genotypes (HEV-5 and HEV-6) infect mainly wild boar. Recently, newly discovered genotypes HEV-7 and HEV-8 were found to infect camels and possibly humans. Nevertheless, the epidemiological distribution of HEV-7 is not well established. HEV-8 is another newly discovered genotype that was identified in 2016 in Chinese Bactrian camels. Although faecal–oral transmission is the most common route of HEV transmission, HEV can be vertically transmitted from infected mothers to their fetuses. HEV may also spread by zoonotic transmission from infected animals to humans and through person-to-person contact. Nowadays, since the number of reported cases linked to blood donations is increasing annually, HEV is recognized as a transfusion-transmitted virus. Laboratory diagnostic techniques vary in their specificity and sensitivity for HEV detection. Direct techniques allow for detection of the viral proteins, antigens and viral nucleic acid, while HEV-specific IgG and IgM antibodies can help establish a diagnosis in acute and chronic infections. In this review, we will discuss recent technologies in the laboratory diagnosis of HEV, including serological and molecular methods to assess the specificity and sensitivity of currently available HEV commercial assays.

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2018-04-01
2019-12-11
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References

  1. CDC Viral Hepatitis – Hepatitis E Information. www.cdc.gov/hepatitis/hev/
  2. WHO 2013; Regional strategy for the prevention and control of viral hepatitis. www.searo.who.int/entity/emerging_diseases/topics/CD_282.pdf
  3. ICTV: Hepeviridae Study Group 2016;https://talk.ictvonline.org/ictv_wikis/hepeviridae
  4. Emerson SU, Clemente-Casares P, Moiduddin N, Arankalle VA, Torian U et al. Putative neutralization epitopes and broad cross-genotype neutralization of Hepatitis E virus confirmed by a quantitative cell-culture assay. J Gen Virol 2006;87:697–704 [CrossRef][PubMed]
    [Google Scholar]
  5. Engle RE, Yu C, Emerson SU, Meng XJ, Purcell RH. Hepatitis E virus (HEV) capsid antigens derived from viruses of human and swine origin are equally efficient for detecting anti-HEV by enzyme immunoassay. J Clin Microbiol 2002;40:4576–4580 [CrossRef][PubMed]
    [Google Scholar]
  6. Delarocque-Astagneau E, Abravanel F, Moshen A, Le Fouler L, Gad RR et al. Epidemiological and virological characteristics of symptomatic acute hepatitis E in Greater Cairo, Egypt. Clin Microbiol Infect 2012;18:982–988 [CrossRef][PubMed]
    [Google Scholar]
  7. El-Sayed Zaki M, El-Deen Zaghloul MH, El Sayed O. Acute sporadic hepatitis E in children: diagnostic relevance of specific immunoglobulin M and immunoglobulin G compared with nested reverse transcriptase PCR. FEMS Immunol Med Microbiol 2006;48:16–20 [CrossRef][PubMed]
    [Google Scholar]
  8. Centers for Disease Control and Prevention (CDC) Investigation of hepatitis E outbreak among refugees – Upper Nile, South Sudan, 2012–2013. MMWR Morb Mortal Wkly Rep 2013;62:581[PubMed]
    [Google Scholar]
  9. International Federation of Red Cross And Red Crescent Societies 2014; Uganda: Hepatitis E Virus disease outbreak DREF Operation n° MDRUG036-Emergency Plan of Action (EPoA) Final Report. http://reliefweb.int/report/uganda/uganda-hepatitis-e-virus-disease-outbreak-dref-operation-n-mdrug036-emergency-plan-0
  10. Teshale EH, Grytdal SP, Howard C, Barry V, Kamili S et al. Evidence of person-to-person transmission of hepatitis E virus during a large outbreak in Northern Uganda. Clin Infect Dis 2010;50:1006–1010 [CrossRef][PubMed]
    [Google Scholar]
  11. Huang CC, Nguyen D, Fernandez J, Yun KY, Fry KE et al. Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology 1992;191:550–558 [CrossRef][PubMed]
    [Google Scholar]
  12. Bi SL, Purdy MA, McCaustland KA, Margolis HS, Bradley DW. The sequence of hepatitis E virus isolated directly from a single source during an outbreak in China. Virus Res 1993;28:233–247[PubMed][Crossref]
    [Google Scholar]
  13. Labrique AB, Thomas DL, Stoszek SK, Nelson KE. Hepatitis E: an emerging infectious disease. Epidemiol Rev 1999;21:162–179 [CrossRef][PubMed]
    [Google Scholar]
  14. Kamar N, Dalton HR, Abravanel F, Izopet J. Hepatitis E virus infection. Clin Microbiol Rev 2014;27:116–138 [CrossRef][PubMed]
    [Google Scholar]
  15. Cao D, Meng XJ. Molecular biology and replication of hepatitis E virus. Emerg Microbes Infect 2012;1:e17 [CrossRef][PubMed]
    [Google Scholar]
  16. Kantala T. Presence of hepatitis E virus (HEV) and markers for HEV infection in production swine, human patients with unexplained hepatitis, and veterinarians in Finland. Dissertationes Schola Doctoralis Scientiae Circumiectalis, Alimentarie, Biologicae. 2017
  17. Woo PC, Lau SK, Teng JL, Tsang AK, Joseph M et al. New hepatitis E virus genotype in camels, the Middle East. Emerg Infect Dis 2014;20:1044–1048 [CrossRef][PubMed]
    [Google Scholar]
  18. Rasche A, Saqib M, Liljander AM, Bornstein S, Zohaib A et al. Hepatitis E Virus Infection in Dromedaries, North and East Africa, United Arab Emirates, and Pakistan, 1983–2015. Emerg Infect Dis 2016;22:1249–1252 [CrossRef][PubMed]
    [Google Scholar]
  19. Woo PC, Lau SK, Teng JL, Cao KY, Wernery U et al. New Hepatitis E virus genotype in bactrian camels, Xinjiang, China, 2013. Emerg Infect Dis 2016;22:2219–2221 [CrossRef][PubMed]
    [Google Scholar]
  20. Aggarwal R. Diagnosis of hepatitis E. Nat Rev Gastroenterol Hepatol 2013;10:24–33 [CrossRef][PubMed]
    [Google Scholar]
  21. Taherkhani R, Farshadpour F. Epidemiology of hepatitis E in pregnant women and children in Iran: a general overview. J Clin Transl Hepatol 2016;4:269–276 [CrossRef][PubMed]
    [Google Scholar]
  22. El Sayed Zaki M, El Razek MM, El Razek HM. Maternal-fetal hepatitis E transmission: is it underestimated?. J Clin Transl Hepatol 2014;2:117–123 [CrossRef][PubMed]
    [Google Scholar]
  23. Abravanel F, Lhomme S, Rostaing L, Kamar N, Izopet J. Protracted fecal shedding of HEV during ribavirin therapy predicts treatment relapse. Clin Infect Dis 2014;60:96–99 [CrossRef][PubMed]
    [Google Scholar]
  24. Khuroo MS, Kamili S. Association of severity of hepatitis E virus infection in the mother and vertically transmitted infection in the fetus. JK Pract 2006;13:70–74
    [Google Scholar]
  25. Blasco-Perrin H, Madden RG, Stanley A, Crossan C, Hunter JG et al. Hepatitis E virus in patients with decompensated chronic liver disease: a prospective UK/French study. Aliment Pharmacol Ther 2015;42:574–581 [CrossRef][PubMed]
    [Google Scholar]
  26. Boxall E, Herborn A, Kochethu G, Pratt G, Adams D et al. Transfusion-transmitted hepatitis E in a 'nonhyperendemic' country. Transfus Med 2006;16:79–83 [CrossRef][PubMed]
    [Google Scholar]
  27. Colson P, Coze C, Gallian P, Henry M, de Micco P et al. Transfusion-associated hepatitis E, France. Emerg Infect Dis 2007;13:648–649 [CrossRef][PubMed]
    [Google Scholar]
  28. Khuroo MS, Kamili S, Yattoo GN. Hepatitis E virus infection may be transmitted through blood transfusions in an endemic area. J Gastroenterol Hepatol 2004;19:778–784 [CrossRef][PubMed]
    [Google Scholar]
  29. Matsubayashi K, Kang JH, Sakata H, Takahashi K, Shindo M et al. A case of transfusion-transmitted hepatitis E caused by blood from a donor infected with hepatitis E virus via zoonotic food-borne route. Transfusion 2008;48:1368–1375 [CrossRef][PubMed]
    [Google Scholar]
  30. Matsubayashi K, Nagaoka Y, Sakata H, Sato S, Fukai K et al. Transfusion-transmitted hepatitis E caused by apparently indigenous hepatitis E virus strain in Hokkaido, Japan. Transfusion 2004;44:934–940 [CrossRef][PubMed]
    [Google Scholar]
  31. Mitsui T, Tsukamoto Y, Yamazaki C, Masuko K, Tsuda F et al. Prevalence of hepatitis E virus infection among hemodialysis patients in Japan: evidence for infection with a genotype 3 HEV by blood transfusion. J Med Virol 2004;74:563–572 [CrossRef][PubMed]
    [Google Scholar]
  32. Tamura A, Shimizu YK, Tanaka T, Kuroda K, Arakawa Y et al. Persistent infection of hepatitis E virus transmitted by blood transfusion in a patient with T-cell lymphoma. Hepatol Res 2007;37:113–120 [CrossRef][PubMed]
    [Google Scholar]
  33. Recommendations from the expert advisory committee on the Safety of Blood, Tissues and Organs (SaBTO) on measures to protect patients from acquiring hepatitis E virus via transfusion or transplantation. 2016;http://hospital.blood.co.uk/media/28794/sabto-recommendations-nov-2016.pdf
  34. Purcell RH, Emerson SU. Hepatitis E: an emerging awareness of an old disease. J Hepatol 2008;48:494–503 [CrossRef][PubMed]
    [Google Scholar]
  35. Féray C, Pawlotsky JM, Roque-Afonso AM, Samuel D, Dhumeaux D. Should we screen blood products for hepatitis E virus RNA ?. Lancet 2014;383:218 [CrossRef][PubMed]
    [Google Scholar]
  36. Vollmer T, Knabbe C, Dreier J. Comparison of real-time PCR and antigen assays for detection of hepatitis E virus in blood donors. J Clin Microbiol 2014;52:2150–2156 [CrossRef][PubMed]
    [Google Scholar]
  37. Gupta E, Pandey P, Pandey S, Sharma MK, Sarin SK. Role of hepatitis E virus antigen in confirming active viral replication in patients with acute viral hepatitis E infection. J Clin Virol 2013;58:374–377 [CrossRef][PubMed]
    [Google Scholar]
  38. Hewitt PE, Ijaz S, Brailsford SR, Brett R, Dicks S et al. Hepatitis E virus in blood components: a prevalence and transmission study in southeast England. Lancet 2014;384:1766–1773 [CrossRef][PubMed]
    [Google Scholar]
  39. Halbur PG, Kasorndorkbua C, Gilbert C, Guenette D, Potters MB et al. Comparative pathogenesis of infection of pigs with hepatitis E viruses recovered from a pig and a human. J Clin Microbiol 2001;39:918–923 [CrossRef][PubMed]
    [Google Scholar]
  40. Billam P, Huang FF, Sun ZF, Pierson FW, Duncan RB et al. Systematic pathogenesis and replication of avian hepatitis E virus in specific-pathogen-free adult chickens. J Virol 2005;79:3429–3437 [CrossRef][PubMed]
    [Google Scholar]
  41. Nan Y, Zhang YJ. Molecular biology and infection of hepatitis E virus. Front Microbiol 2016;7:1419 [CrossRef][PubMed]
    [Google Scholar]
  42. Aggarwal R, Goel A. Advances in hepatitis E - I: virology, pathogenesis and diagnosis. Expert Rev Gastroenterol Hepatol 2016;10:1053–1063 [CrossRef][PubMed]
    [Google Scholar]
  43. Zhang F, Li X, Li Z, Harrison TJ, Chong H et al. Detection of HEV antigen as a novel marker for the diagnosis of hepatitis E. J Med Virol 2006;78:1441–1448 [CrossRef][PubMed]
    [Google Scholar]
  44. Takahashi M, Yamada K, Hoshino Y, Takahashi H, Ichiyama K et al. Monoclonal antibodies raised against the ORF3 protein of hepatitis E virus (HEV) can capture HEV particles in culture supernatant and serum but not those in feces. Arch Virol 2008;153:1703–1713 [CrossRef][PubMed]
    [Google Scholar]
  45. Aggarwal R, Kini D, Sofat S, Naik SR, Krawczynski K. Duration of viraemia and faecal viral excretion in acute hepatitis E. Lancet 2000;356:1081–1082 [CrossRef][PubMed]
    [Google Scholar]
  46. Geng Y, Zhao C, Huang W, Harrison TJ, Zhang H et al. Detection and assessment of infectivity of hepatitis E virus in urine. J Hepatol 2016;64:37–43 [CrossRef][PubMed]
    [Google Scholar]
  47. Trémeaux P, Lhomme S, Chapuy-Regaud S, Peron JM, Alric L et al. Performance of an antigen assay for diagnosing acute hepatitis E virus genotype 3 infection. J Clin Virol 2016;79:1–5 [CrossRef][PubMed]
    [Google Scholar]
  48. Park HK, Jeong SH, Kim JW, Woo BH, Lee DH et al. Seroprevalence of anti-hepatitis E virus (HEV) in a Korean population: comparison of two commercial anti-HEV assays. BMC Infect Dis 2012;12:142 [CrossRef][PubMed]
    [Google Scholar]
  49. Kmush BL, Labrique AB, Dalton HR, Ahmed ZB, Ticehurst JR et al. Two generations of "Gold Standards": The impact of a decade in hepatitis E virus testing innovation on population seroprevalence. Am J Trop Med Hyg 2015;93:714–717 [CrossRef][PubMed]
    [Google Scholar]
  50. Vernier M, Rossi-Tamisier M, Richet H, Brouqui P, Parola P et al. Anti-hepatitis E virus antibody prevalence in French expatriate workers. Int J Infect Dis 2013;17:e1082-4 [CrossRef][PubMed]
    [Google Scholar]
  51. Bendall R, Ellis V, Ijaz S, Ali R, Dalton H. A comparison of two commercially available anti-HEV IgG kits and a re-evaluation of anti-HEV IgG seroprevalence data in developed countries. J Med Virol 2010;82:799–805 [CrossRef][PubMed]
    [Google Scholar]
  52. Avellon A, Morago L, Garcia-Galera del Carmen M, Munoz M, Echevarría JM. Comparative sensitivity of commercial tests for hepatitis E genotype 3 virus antibody detection. J Med Virol 2015;87:1934–1939 [CrossRef][PubMed]
    [Google Scholar]
  53. Pas SD, Streefkerk RH, Pronk M, de Man RA, Beersma MF et al. Diagnostic performance of selected commercial HEV IgM and IgG ELISAs for immunocompromised and immunocompetent patients. J Clin Virol 2013;58:629–634 [CrossRef][PubMed]
    [Google Scholar]
  54. Abravanel F, Chapuy-Regaud S, Lhomme S, Miedougé M, Peron JM et al. Performance of anti-HEV assays for diagnosing acute hepatitis E in immunocompromised patients. J Clin Virol 2013;58:624–628 [CrossRef][PubMed]
    [Google Scholar]
  55. Schnegg A, Bürgisser P, André C, Kenfak-Foguena A, Canellini G et al. An analysis of the benefit of using HEV genotype 3 antigens in detecting anti-HEV IgG in a European population. PLoS One 2013;8:e62980 [CrossRef][PubMed]
    [Google Scholar]
  56. Sommerkorn FM, Schauer B, Schreiner T, Fickenscher H, Krumbholz A. Performance of hepatitis E virus (HEV)-antibody tests: a comparative analysis based on samples from individuals with direct contact to domestic pigs or wild boar in Germany. Med Microbiol Immunol 2017;206:277–286 [CrossRef][PubMed]
    [Google Scholar]
  57. Favorov MO, Fields HA, Purdy MA, Yashina TL, Aleksandrov AG et al. Serologic identification of hepatitis E virus infections in epidemic and endemic settings. J Med Virol 1992;36:246–250 [CrossRef][PubMed]
    [Google Scholar]
  58. Herremans M, Duizer E, Jusic E, Koopmans MP. Detection of hepatitis E virus-specific immunoglobulin a in patients infected with hepatitis E virus genotype 1 or 3. Clin Vaccine Immunol 2007;14:276–280 [CrossRef][PubMed]
    [Google Scholar]
  59. Zhang S, Tian D, Zhang Z, Xiong J, Yuan Q et al. Clinical significance of anti-HEV IgA in diagnosis of acute genotype 4 hepatitis E virus infection negative for anti-HEV IgM. Dig Dis Sci 2009;54:2512–2518 [CrossRef][PubMed]
    [Google Scholar]
  60. Takahashi M, Kusakai S, Mizuo H, Suzuki K, Fujimura K et al. Simultaneous detection of immunoglobulin A (IgA) and IgM antibodies against hepatitis E virus (HEV) Is highly specific for diagnosis of acute HEV infection. J Clin Microbiol 2005;43:49–56 [CrossRef][PubMed]
    [Google Scholar]
  61. Khudyakov YE, Favorov MO, Khudyakova NS, Cong ME, Holloway BP et al. Artificial mosaic protein containing antigenic epitopes of hepatitis E virus. J Virol 1994;68:7067–7074[PubMed]
    [Google Scholar]
  62. Mast EE, Alter MJ, Holland PV, Purcell RH. Evaluation of assays for antibody to hepatitis E virus by a serum panel. Hepatitis E virus antibody serum panel evaluation group. Hepatology 1998;27:857–861 [CrossRef][PubMed]
    [Google Scholar]
  63. Lin CC, Wu JC, Chang TT, Chang WY, Yu ML et al. Diagnostic value of immunoglobulin G (IgG) and IgM anti-hepatitis E virus (HEV) tests based on HEV RNA in an area where hepatitis E is not endemic. J Clin Microbiol 2000;38:3915–3918[PubMed]
    [Google Scholar]
  64. Shrestha AC, Flower RL, Seed CR, Stramer SL, Faddy HM. A comparative study of assay performance of commercial hepatitis E virus enzyme-linked immunosorbent assay kits in Australian blood donor samples. J Blood Transfus 2016;2016:1–6 [CrossRef][PubMed]
    [Google Scholar]
  65. Hyams C, Mabayoje DA, Copping R, Maranao D, Patel M et al. Serological cross reactivity to CMV and EBV causes problems in the diagnosis of acute hepatitis E virus infection. J Med Virol 2014;86:478–483 [CrossRef][PubMed]
    [Google Scholar]
  66. Chen HY, Lu Y, Howard T, Anderson D, Fong PY et al. Comparison of a new immunochromatographic test to enzyme-linked immunosorbent assay for rapid detection of immunoglobulin m antibodies to hepatitis e virus in human sera. Clin Diagn Lab Immunol 2005;12:593–598 [CrossRef][PubMed]
    [Google Scholar]
  67. Legrand-Abravanel F, Thevenet I, Mansuy JM, Saune K, Vischi F et al. Good performance of immunoglobulin M assays in diagnosing genotype 3 hepatitis E virus infections. Clin Vaccine Immunol 2009;16:772–774 [CrossRef][PubMed]
    [Google Scholar]
  68. Myint KS, Guan M, Chen HY, Lu Y, Anderson D et al. Evaluation of a new rapid immunochromatographic assay for serodiagnosis of acute hepatitis E infection. Am J Trop Med Hyg 2005;73:942–946[PubMed]
    [Google Scholar]
  69. Vollmer T, Diekmann J, Eberhardt M, Knabbe C, Dreier J. Monitoring of anti-hepatitis E virus antibody seroconversion in asymptomatically infected blood donors: systematic comparison of nine commercial anti-HEV IgM and IgG assays. Viruses 2016;8:232 [CrossRef]
    [Google Scholar]
  70. Yu C, Engle RE, Bryan JP, Emerson SU, Purcell RH. Detection of immunoglobulin M antibodies to hepatitis E virus by class capture enzyme immunoassay. Clin Diagn Lab Immunol 2003;10:579–586 [CrossRef][PubMed]
    [Google Scholar]
  71. Wenzel JJ, Preiss J, Schemmerer M, Huber B, Jilg W. Test performance characteristics of Anti-HEV IgG assays strongly influence hepatitis E seroprevalence estimates. J Infect Dis 2013;207:497–500 [CrossRef][PubMed]
    [Google Scholar]
  72. Kodani M, Kamili NA, Tejada-Strop A, Poe A, Denniston MM et al. Variability in the performance characteristics of IgG anti-HEV assays and its impact on reliability of seroprevalence rates of hepatitis E. J Med Virol 2017;89:1055–1061 [CrossRef][PubMed]
    [Google Scholar]
  73. Rossi-Tamisier M, Moal V, Gerolami R, Colson P. Discrepancy between anti-hepatitis E virus immunoglobulin G prevalence assessed by two assays in kidney and liver transplant recipients. J Clin Virol 2013;56:62–64 [CrossRef][PubMed]
    [Google Scholar]
  74. Allain JP. Genomic screening for blood-borne viruses in transfusion settings. Clin Lab Haematol 2000;22:1–10 [CrossRef][PubMed]
    [Google Scholar]
  75. Pawlotsky JM. Hepatitis E screening for blood donations: an urgent need?. Lancet 2014;384:1729–1730 [CrossRef][PubMed]
    [Google Scholar]
  76. Chandnani M, Kaur M, Ramadhas A, Tumarinson T. A case report about the most common yet most forgotten hepatitis E. Am J Case Rep 2016;17:584–586 [CrossRef][PubMed]
    [Google Scholar]
  77. Dawson GJ, Mushahwar IK, Chau KH, Gitnick GL. Detection of long-lasting antibody to hepatitis E virus in a US traveller to Pakistan. Lancet 1992;340:426–427 [CrossRef][PubMed]
    [Google Scholar]
  78. Nasrallah GK, Al Absi ES, Ghandour R, Ali NH, Taleb S et al. Seroprevalence of hepatitis E virus among blood donors in Qatar (2013–2016). Transfusion 2017;57:1801–1807 [CrossRef][PubMed]
    [Google Scholar]
  79. Vollmer T, Diekmann J, Johne R, Eberhardt M, Knabbe C et al. Novel approach for detection of hepatitis E virus infection in German blood donors. J Clin Microbiol 2012;50:2708–2713 [CrossRef][PubMed]
    [Google Scholar]
  80. Fischer C, Hofmann M, Danzer M, Hofer K, Kaar J et al. Seroprevalence and Incidence of hepatitis E in blood donors in upper Austria. PLoS One 2015;10:e0119576 [CrossRef][PubMed]
    [Google Scholar]
  81. Behrendt P, Bremer B, Todt D, Brown RJ, Heim A et al. Hepatitis E virus (HEV) ORF2 antigen levels differentiate between acute and chronic HEV infection. J Infect Dis 2016;214:361–368 [CrossRef][PubMed]
    [Google Scholar]
  82. Pischke S, Suneetha PV, Baechlein C, Barg-Hock H, Heim A et al. Hepatitis E virus infection as a cause of graft hepatitis in liver transplant recipients. Liver Transpl 2010;16:74–82 [CrossRef][PubMed]
    [Google Scholar]
  83. Coilly A, Haïm-Boukobza S, Roche B, Antonini TM, Pause A et al. Posttransplantation hepatitis E: transfusion-transmitted hepatitis rising from the ashes. Transplantation 2013;96:e4-e6 [CrossRef][PubMed]
    [Google Scholar]
  84. Sakata H, Matsubayashi K, Takeda H, Sato S, Kato T et al. A nationwide survey for hepatitis E virus prevalence in Japanese blood donors with elevated alanine aminotransferase. Transfusion 2008;48:2568–2576 [CrossRef][PubMed]
    [Google Scholar]
  85. Guo QS, Yan Q, Xiong JH, Ge SX, Shih JW et al. Prevalence of hepatitis E virus in Chinese blood donors. J Clin Microbiol 2010;48:317–318 [CrossRef][PubMed]
    [Google Scholar]
  86. Petrik J, Lozano M, Seed CR, Faddy HM, Keller AJ et al. Hepatitis E. Vox Sang 2016;110:93–103 [CrossRef][PubMed]
    [Google Scholar]
  87. Adlhoch C, Kaiser M, Pauli G, Koch J, Meisel H. Indigenous hepatitis E virus infection of a plasma donor in Germany. Vox Sang 2009;97:303–308 [CrossRef][PubMed]
    [Google Scholar]
  88. Ijaz S, Szypulska R, Tettmar KI, Kitchen A, Tedder RS. Detection of hepatitis E virus RNA in plasma mini-pools from blood donors in England. Vox Sang 2011;102:272 [CrossRef][PubMed]
    [Google Scholar]
  89. Baylis SA, Gärtner T, Nick S, Ovemyr J, Blümel J. Occurrence of hepatitis E virus RNA in plasma donations from Sweden, Germany and the United States. Vox Sang 2012;103:89–90 [CrossRef][PubMed]
    [Google Scholar]
  90. Cleland A, Smith L, Crossan C, Blatchford O, Dalton HR et al. Hepatitis E virus in Scottish blood donors. Vox Sang 2013;105:283–289 [CrossRef][PubMed]
    [Google Scholar]
  91. Corman VM, Drexler JF, Eckerle I, Roth WK, Drosten C et al. Zoonotic hepatitis E virus strains in German blood donors. Vox Sang 2013;104:179–180 [CrossRef][PubMed]
    [Google Scholar]
  92. Gallian P, Lhomme S, Piquet Y, Sauné K, Abravanel F et al. Hepatitis E virus infections in blood donors, France. Emerg Infect Dis 2014;20:1914–1917 [CrossRef][PubMed]
    [Google Scholar]
  93. Sauleda S, Ong E, Bes M, Janssen A, Cory R et al. Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription-mediated amplification assay in blood donors from Catalonia (Spain). Transfusion 2015;55:972–979 [CrossRef][PubMed]
    [Google Scholar]
  94. Mine H, Emura H, Miyamoto M, Tomono T, Minegishi K et al. High throughput screening of 16 million serologically negative blood donors for hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1 by nucleic acid amplification testing with specific and sensitive multiplex reagent in Japan. J Virol Methods 2003;112:145–151 [CrossRef][PubMed]
    [Google Scholar]
  95. Tavares RG, Staggemeier R, Borges ALP, Rodrigues MT, Castelan LA et al. Molecular techniques for the study and diagnosis of parasite infection. J Venom Anim Toxins Incl Trop Dis 2011;17:239–248 [CrossRef]
    [Google Scholar]
  96. Gallarda JL, Dragon E. Blood screening by nucleic acid amplification technology: current issues, future challenges. Mol Diagn 2000;5:11–22 [CrossRef][PubMed]
    [Google Scholar]
  97. Vyas GN, Yang G, Murphy EL. Transfusion-related transmissible diseases: detection by polymerase chain reaction-amplified genes of the microbial agents. Transfus Med Rev 1994;8:253–266 [CrossRef][PubMed]
    [Google Scholar]
  98. Deepak S, Kottapalli K, Rakwal R, Oros G, Rangappa K et al. Real-Time PCR: revolutionizing detection and expression analysis of genes. Curr Genomics 2007;8:234–251 [CrossRef][PubMed]
    [Google Scholar]
  99. Pripuzova N, Wang R, Tsai S, Li B, Hung GC et al. Development of real-time PCR array for simultaneous detection of eight human blood-borne viral pathogens. PLoS One 2012;7:e43246 [CrossRef][PubMed]
    [Google Scholar]
  100. Gerber PF, Xiao CT, Cao D, Meng XJ, Opriessnig T. Comparison of real-time reverse transcriptase PCR assays for detection of swine hepatitis E virus in fecal samples. J Clin Microbiol 2014;52:1045–1051 [CrossRef][PubMed]
    [Google Scholar]
  101. Zhao Q, Xie S, Sun Y, Chen Y, Gao J et al. Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus. BMC Vet Res 2015;11:195 [CrossRef][PubMed]
    [Google Scholar]
  102. Stensvold CR, Ahmed UN, Andersen LO, Nielsen HV. Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp. J Clin Microbiol 2012;50:1847–1851 [CrossRef][PubMed]
    [Google Scholar]
  103. Pestana E, Belak S, Diallo A, Crowther JR, Viljoen GJ et al. Early, Rapid and Sensitive Veterinary Molecular Diagnostics-Real Time PCR Applications Netherlands: Springer Science & Business Media; 2010;[Crossref]
    [Google Scholar]
  104. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J et al. The real-time polymerase chain reaction. Mol Aspects Med 2006;27:95–125 [CrossRef][PubMed]
    [Google Scholar]
  105. Chhabra P, Vinjé J. Molecular detection methods of foodborne viruses. In Goyal S, Cannon J. (editors) Viruses in Foods Cham: Springer; 2016; pp.303–333[Crossref]
    [Google Scholar]
  106. Acquisition of CEERAM, specialized in the detection of foodborne viruses. www.biomerieux-industry.com/acquisition-ceeram-specialized-detection-foodborne-viruses
  107. Pelosi E, Clarke I. Hepatitis E: a complex and global disease. Emerg Health Threats J 2008;1:e8 [CrossRef][PubMed]
    [Google Scholar]
  108. Nascimento MSJ, Abreu-Silva J, Teixeira J, Oliveira RMS, Mesquita JR et al. Seroprevalence of hepatitis E virus among the Portuguese general population and prevalence of silent infection in blood donors – HEPeCONTROL. J Clin Virol 2016;82:S14–S15 [CrossRef]
    [Google Scholar]
  109. Juhl D, Baylis SA, Blümel J, Görg S, Hennig H. Seroprevalence and incidence of hepatitis E virus infection in German blood donors. Transfusion 2014;54:49–56 [CrossRef][PubMed]
    [Google Scholar]
  110. Baylis SA, Blümel J, Mizusawa S, Matsubayashi K, Sakata H et al. International standard to harmonize assays for detection of hepatitis E virus RNA. 2013
  111. Baylis SA, Hanschmann KM, Blümel J, Nübling CM. Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance. J Clin Microbiol 2011;49:1234–1239 [CrossRef][PubMed]
    [Google Scholar]
  112. Eiken The principle of LAMP method. http://loopamp.eiken.co.jp/e/lamp/principle.html
  113. Notomi T, Mori Y, Tomita N, Kanda H. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J Microbiol 2015;53:1–5 [CrossRef][PubMed]
    [Google Scholar]
  114. Njiru ZK, Mikosza AS, Armstrong T, Enyaru JC, Ndung'u JM et al. Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense. PLoS Negl Trop Dis 2008;2:e147 [CrossRef][PubMed]
    [Google Scholar]
  115. Meridian Bioscience LAMP technology - description of the lamp process. https://vimeo.com/13391184
  116. Lan X, Yang B, Li BY, Yin XP, Li XR et al. Reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus. J Clin Microbiol 2009;47:2304–2306 [CrossRef][PubMed]
    [Google Scholar]
  117. Hologic 2017; Transcription Mediated Amplification (TMA). www.youtube.com/watch?v=qQQtq1LaGL8
  118. Stramer SL, Moritz ED, Foster GA, Ong E, Linnen JM et al. Hepatitis E virus: seroprevalence and frequency of viral RNA detection among US blood donors. Transfusion 2016;56:481–488 [CrossRef][PubMed]
    [Google Scholar]
  119. Baylis SA, Corman VM, Ong E, Linnen JM, Nübling CM et al. Hepatitis E viral loads in plasma pools for fractionation. Transfusion 2016;56:2532–2537 [CrossRef][PubMed]
    [Google Scholar]
  120. Shrestha AC, Flower RL, Seed CR, Keller AJ, Harley R et al. Hepatitis E virus RNA in Australian blood donations. Transfusion 2016;56:3086–3093 [CrossRef][PubMed]
    [Google Scholar]
  121. O'Riordan J, Boland F, Williams P, Donnellan J, Hogema BM et al. Hepatitis E virus infection in the Irish blood donor population. Transfusion 2016;56:2868–2876 [CrossRef][PubMed]
    [Google Scholar]
  122. La Rosa G, Fratini M, Muscillo M, Iaconelli M, Taffon S et al. Molecular characterisation of human hepatitis E virus from Italy: comparative analysis of five reverse transcription-PCR assays. Virol J 2014;11:72 [CrossRef][PubMed]
    [Google Scholar]
  123. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  124. Lu L, Li C, Hagedorn CH. Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol 2006;16:5–36 [CrossRef][PubMed]
    [Google Scholar]
  125. World Health Organization The global prevalence of hepatitis A virus infection and susceptibility: a systematic review. 2010
  126. Gad YZ, Mousa N, Shams M, Elewa A. Seroprevalence of subclinical HEV infection in asymptomatic, apparently healthy, pregnant women in Dakahlya Governorate, Egypt. Asian J Transfus Sci 2011;5:136 [CrossRef][PubMed]
    [Google Scholar]
  127. Mirazo S, Ramos N, Mainardi V, Gerona S, Arbiza J. Transmission, diagnosis, and management of hepatitis E: an update. Hepat Med 2014;6:45 [CrossRef][PubMed]
    [Google Scholar]
  128. Zhou H, Jiang CW, Li LP, Zhao CY, Wang YC et al. Comparison of the reliability of two ELISA kits for detecting IgM antibody against hepatitis E virus. Zhonghua Yu Fang Yi Xue Za Zhi 2008;42:667–671[PubMed]
    [Google Scholar]
  129. Abravanel F, Lhomme S, Chapuy-Regaud S, Peron JM, Alric L et al. Performance of a new rapid test for detecting anti-hepatitis E virus immunoglobulin M in immunocompetent and immunocompromised patients. J Clin Virol 2015;70:101–104 [CrossRef][PubMed]
    [Google Scholar]
  130. Norder H, Karlsson M, Mellgren Å, Konar J, Sandberg E et al. Diagnostic performance of five assays for anti-hepatitis E virus IgG and IgM in a large cohort study. J Clin Microbiol 2016;54:549–555 [CrossRef][PubMed]
    [Google Scholar]
  131. Drobeniuc J, Meng J, Reuter G, Greene-Montfort T, Khudyakova N et al. Serologic assays specific to immunoglobulin M antibodies against hepatitis E virus: pangenotypic evaluation of performances. Clin Infect Dis 2010;51:e24-e27 [CrossRef][PubMed]
    [Google Scholar]
  132. Wu WC, Su CW, Yang JY, Lin SF, Chen JY et al. Application of serologic assays for diagnosing acute hepatitis E in national surveillance of a nonendemic area. J Med Virol 2014;86:720–728 [CrossRef][PubMed]
    [Google Scholar]
  133. Abravanel F, Chapuy-Regaud S, Lhomme S, Miedougé M, Peron JM et al. Performance of anti-HEV assays for diagnosing acute hepatitis E in immunocompromised patients. J Clin Virol 2013;58:624–628 [CrossRef][PubMed]
    [Google Scholar]
  134. Galiana C, Fernández-Barredo S, García A, Gómez MT, Pérez-Gracia MT. Occupational exposure to hepatitis E virus (HEV) in swine workers. Am J Trop Med Hyg 2008;78:1012–1015[PubMed]
    [Google Scholar]
  135. Schnegg A, Bürgisser P, André C, Kenfak-Foguena A, Canellini G et al. A comparison of three anti-HEV IgG EIA screening kits and one confirmatory immunodot assay in blood donor samples in Switzerland. Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) San Francisco CA, USA: 2012
    [Google Scholar]
  136. Yan Q, Du H-l, Wang Y-b, Ge S-x, Huang S-j et al. Comparison of two diagnostic reagents to detect anti-hepatitis E virus IgG antibodies. Chinese Journal of Zoonoses 2008;24:1087–1089
    [Google Scholar]
  137. Garg G, Kumar D, Asim M, Husain SA, das BC et al. Multiplex reverse transcriptase-PCR for simultaneous detection of hepatitis B, C, and E virus. J Clin Exp Hepatol 2016;6:33–39 [CrossRef][PubMed]
    [Google Scholar]
  138. Meng XJ, Purcell RH, Halbur PG, Lehman JR, Webb DM et al. A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 1997;94:9860–9865 [CrossRef][PubMed]
    [Google Scholar]
  139. Wu JC, Sheen IJ, Chiang TY, Sheng WY, Wang YJ et al. The impact of traveling to endemic areas on the spread of hepatitis E virus infection: epidemiological and molecular analyses. Hepatology 1998;27:1415–1420 [CrossRef][PubMed]
    [Google Scholar]
  140. Jothikumar N, Aparna K, Kamatchiammal S, Paulmurugan R, Saravanadevi S et al. Detection of hepatitis E virus in raw and treated wastewater with the polymerase chain reaction. Appl Environ Microbiol 1993;59:2558–2562[PubMed]
    [Google Scholar]
  141. Pas SD, de Man RA, Mulders C, Balk AH, van Hal PT et al. Hepatitis E virus infection among solid organ transplant recipients, the Netherlands. Emerg Infect Dis 2012;18:869–872 [CrossRef][PubMed]
    [Google Scholar]
  142. Slot E, Hogema BM, Riezebos-Brilman A, Kok TM, Molier M et al. Silent hepatitis E virus infection in Dutch blood donors, 2011 to 2012. Euro Surveill 2013;18:20550 [CrossRef][PubMed]
    [Google Scholar]
  143. Abravanel F, Sandres-Saune K, Lhomme S, Dubois M, Mansuy JM et al. Genotype 3 diversity and quantification of hepatitis E virus RNA. J Clin Microbiol 2012;50:897–902 [CrossRef][PubMed]
    [Google Scholar]
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