1887

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Volume 67, Issue 4

Molecular characterization and antifungal susceptibility testing of from southern Brazil Free

Abstract

Cryptococcosis is acquired from the environment by the inhalation of cells and may establish from an asymptomatic latent infection into pneumonia or meningoencephalitis. The genetic diversity of a species complex has been investigated by several molecular tools, such as multi-locus sequence typing, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and microsatellite analysis. This study aimed to investigate the genotype distributions and antifungal susceptibility profiles of isolates from southern Brazil.

We studied 219 isolates with mating- and serotyping, AFLP fingerprinting, microsatellite typing and antifungal susceptibility testing.

Among the isolates, 136 (69 %) were from HIV-positive patients. Only mating-type α and serotype A were observed. AFLP fingerprinting analysis divided the isolates into AFLP1/VNI (=172; 78.5 %), AFLP1A/VNII (=19; 8.7 %), AFLP1B/VNII (=4; 1.8 %) and a new AFLP pattern AFLP1C (=23; 10.5 %). All isolates were susceptible to tested antifungals and no correlation between antifungal susceptibility and genotypes was observed. Through microsatellite analysis, most isolates clustered in a major microsatellite complex and Simpson’s diversity index of this population was =0.9856.

The majority of infections occurred in HIV-positive patients. AFLP1/VNI was the most frequent genotype and all antifungal drugs had high activity against this species. Microsatellite analyses showed a high genetic diversity within the regional population, and correlation between environmental and clinical isolates, as well as a temporal and geographic relationship.

  • Received:
  • Accepted:
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Keyword(s): amplified fragment length polymorphism , antifungal susceptibility , Cryptococcosis , Cryptococcus neoformans , mating-type and microsatellite typing
© 2018 The Authors
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2018-04-01
2024-04-23
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Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
J. Med. Microbiol. 67, 560 (2018); https://doi.org/10.1099/jmm.0.000698
/content/journal/jmm/10.1099/jmm.0.000698
/content/journal/jmm/10.1099/jmm.0.000698
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