1887

Abstract

Purpose. The decline in methicillin-resistant Staphylococcus aureus (MRSA) isolation rates has become a general observation worldwide, including Japan. We hypothesized that some genetic shift in MRSA might cause this phenomenon, and therefore we investigated the genetic profiles among MRSA clinical isolates obtained from three different epidemic phases in Japan.

Methodology. A total of 353 MRSA isolates were selected from 202 medical facilities in 1990 (pre-epidemic phase), 2004 (epidemic phase) and 2016 (post-epidemic phase). Molecular typing was performed by PCR detection of 22 genes using the polymerase chain reaction (PCR)-based ORF typing (POT) system, including an additional eight genes including small genomic islets and seven toxin genes.

Results. Isolates with a POT1 of score 93, identified as presumed clonal complex (pCC)5-staphylococcal cassette chromosome mec (SCCmec) type II including ST5-SCCmec type II New York/Japan clone, represented the major epidemic MRSA lineage in 1990 and 2004. In 2016, however, a marked decrease in isolates with a POT1 score of 93, along with changes in the epidemiology of toxin genes carried, was noted, where the carriers of tst genes including the tst-sec combination were markedly reduced, and those possessing the seb gene alone were markedly increased. Rather, isolates with a POT1 score of 106, including pCC1 or pCC8 among the isolates with SCCmec type IV, which often links to community-associated MRSA, were predominant. Interestingly, the pCC1 and pCC8 lineages were related to sea and tst-sec carriage, respectively.

Conclusions. Over time, a transition in MRSA genetic profiles from a POT1 score of 93 in 1990 and 2004 to 106 in 2014 was found in Japan.

Keyword(s): clone , east Japan , MRSA , POT and west Japan
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2018-02-02
2019-12-05
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References

  1. Cardo D, Horan T, Andrus M, Dembinski M, Edwards J et al. National nosocomial infections surveillance (NNIS) system report, data summary from January 1992 through June 2004, issued October 2004. Am J Infect Control 2004;32:470–485 [CrossRef][PubMed]
    [Google Scholar]
  2. Christiansen KJ, Bell JM, Turnidge JD, Jones RN. Antimicrobial activities of garenoxacin (BMS 284756) against Asia-Pacific region clinical isolates from the SENTRY program, 1999 to 2001. Antimicrob Agents Chemother 2004;48:2049–2055 [CrossRef][PubMed]
    [Google Scholar]
  3. David MZ, Daum RS. Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clin Microbiol Rev 2010;23:616–687 [CrossRef][PubMed]
    [Google Scholar]
  4. Seybold U, Kourbatova EV, Johnson JG, Halvosa SJ, Wang YF et al. Emergence of community-associated methicillin-resistant Staphylococcus aureus USA300 genotype as a major cause of health care-associated blood stream infections. Clin Infect Dis 2006;42:647–656 [CrossRef][PubMed]
    [Google Scholar]
  5. Kallen AJ, Mu Y, Bulens S, Reingold A, Petit S et al. Health care-associated invasive MRSA infections, 2005-2008. JAMA 2010;304:641–648 [CrossRef][PubMed]
    [Google Scholar]
  6. Chen CJ, Huang YC. New epidemiology of Staphylococcus aureus infection in Asia. Clin Microbiol Infect 2014;20:605–623 [CrossRef][PubMed]
    [Google Scholar]
  7. Mukae H, Iwamoto M, Takase T, Moro N, Ishino T et al. Isolation of methicillin-resistant Staphylococcus aureus (MRSA) at the Hokusho Central Hospital – observation during the recent 4 years. Kansenshogaku Zasshi 1990;64:1275–1286 [CrossRef][PubMed]
    [Google Scholar]
  8. Kimura A, Igarashi H, Ushioda H, Okuzumi K, Kobayashi H et al. Epidemiological study of Staphylococcus aureus isolated from the Japanese National University and Medical College Hospitals with coagulase typing, and production of enterotoxins and toxic shock syndrome toxin-1. Kansenshogaku Zasshi 1992;66:1543–1549 [CrossRef][PubMed]
    [Google Scholar]
  9. Ministry of Health Labour and Welfare The Japan nosocomial infections surveillance. Available athttps://janis.mhlw.go.jp/english/index.asp [Accessed 27 August 2017]
  10. Suzuki M, Tawada Y, Kato M, Hori H, Mamiya N et al. Development of a rapid strain differentiation method for methicillin-resistant Staphylococcus aureus isolated in Japan by detecting phage-derived open-reading frames. J Appl Microbiol 2006;101:938–947 [CrossRef][PubMed]
    [Google Scholar]
  11. Suzuki M, Matsumoto M, Takahashi M, Hayakawa Y, Minagawa H. Identification of the clonal complexes of Staphylococcus aureus strains by determination of the conservation patterns of small genomic islets. J Appl Microbiol 2009;107:1367–1374 [CrossRef][PubMed]
    [Google Scholar]
  12. Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H et al. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect Immun 2002;70:631–641 [CrossRef][PubMed]
    [Google Scholar]
  13. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol 2000;38:1032–1035[PubMed]
    [Google Scholar]
  14. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 2006;23:254–267 [CrossRef][PubMed]
    [Google Scholar]
  15. Maeda T, Saga T, Miyazaki T, Kouyama Y, Harada S et al. Genotyping of skin and soft tissue infection (SSTI)-associated methicillin-resistant Staphylococcus aureus (MRSA) strains among outpatients in a teaching hospital in Japan: application of a phage-open reading frame typing (POT) kit. J Infect Chemother 2012;18:906–914 [CrossRef][PubMed]
    [Google Scholar]
  16. O'Sullivan MV, Kong F, Sintchenko V, Gilbert GL. Rapid identification of methicillin-resistant Staphylococcus aureus transmission in hospitals by use of phage-derived open reading frame typing enhanced by multiplex PCR and reverse line blot assay. J Clin Microbiol 2010;48:2741–2748 [CrossRef][PubMed]
    [Google Scholar]
  17. Köck R, Becker K, Cookson B, van Gemert-Pijnen JE, Harbarth S et al. Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Euro Surveill 2010;15:19688 [CrossRef][PubMed]
    [Google Scholar]
  18. Evans ME, Kralovic SM, Simbartl LA, Jain R, Roselle GA. Eight years of decreased methicillin-resistant Staphylococcus aureus health care-associated infections associated with a Veterans Affairs prevention initiative. Am J Infect Control 2017;45:13–16 [CrossRef][PubMed]
    [Google Scholar]
  19. Huang YC, Lien RI, Su LH, Chou YH, Lin TY. Successful control of methicillin-resistant Staphylococcus aureus in endemic neonatal intensive care units – a 7-year campaign. PLoS One 2011;6:e23001 [CrossRef][PubMed]
    [Google Scholar]
  20. Popovich KJ, Weinstein RA, Hota B. Are community-associated methicillin-resistant Staphylococcus aureus (MRSA) strains replacing traditional nosocomial MRSA strains?. Clin Infect Dis 2008;46:787–794 [CrossRef][PubMed]
    [Google Scholar]
  21. Oliveira DC, Tomasz A, de Lencastre H. Secrets of success of a human pathogen: molecular evolution of pandemic clones of meticillin-resistant Staphylococcus aureus. Lancet Infect Dis 2002;2:180–189 [CrossRef][PubMed]
    [Google Scholar]
  22. Takano T, Hung WC, Shibuya M, Higuchi W, Iwao Y et al. A new local variant (ST764) of the globally disseminated ST5 lineage of hospital-associated methicillin-resistant Staphylococcus aureus (MRSA) carrying the virulence determinants of community-associated MRSA. Antimicrob Agents Chemother 2013;57:1589–1595 [CrossRef][PubMed]
    [Google Scholar]
  23. Isobe H, Takano T, Nishiyama A, Hung WC, Kuniyuki S et al. Evolution and virulence of Panton-Valentine leukocidin-positive ST30 methicillin-resistant Staphylococcus aureus in the past 30 years in Japan. Biomed Res 2012;33:97–109 [CrossRef][PubMed]
    [Google Scholar]
  24. Yamaguchi T, Okamura S, Miura Y, Koyama S, Yanagisawa H et al. Molecular characterization of community-associated methicillin-resistant Staphylococcus aureus isolated from skin and pus samples of outpatients in Japan. Microb Drug Resist 2015;21:441–447 [CrossRef][PubMed]
    [Google Scholar]
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