1887

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Volume 67, Issue 3

Isolation, characterization and genomic analysis of a novel lytic bacteriophage vB_SsoS-ISF002 infecting and Free

Abstract

Shigellosis is one of the most important food-borne and water-borne diseases worldwide. Although antibiotics are considered as efficient agents for shigellosis treatment, improper use of these has led to the emergence of antibiotic-resistant spp. Therefore, finding a new strategy as alternative treatment seems necessary.

Different samples from a wastewater treatment plant were used to isolate spp. specific phages. Physiological properties were determined, and genomic analysis was also carried out.

A virulent bacteriophage, vB_SsoS-ISF002, was isolated from urban wastewater in Iran and showed infectivity to different isolates of both and . vB_SsoS-ISF002 was stable at different pH values and temperatures. It had a short latent period (15 min), a large burst size (76±9 p.f.u. cell) and appropriate lytic activity especially at high MOI. Its genome (dsDNA) was 50 564 bp with 45.53 % GC content and 76 predicted open reading frames. According to comparative genomic analysis and phylogenic tree construction, vB_SsoS-ISF002 was considered as a member of the genus.

These results indicated that vB_SsoS-ISF002 is a novel virulent phage and may have potential as an alternative treatment for shigellosis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): phage therapy , Shigella flexneri , Shigella sonnei , Shigellosis , Siphoviridae and T1virus
© 2018 The Authors
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2018-03-01
2024-04-19
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Isolation, characterization and genomic analysis of a novel lytic bacteriophage vB_SsoS-ISF002 infecting Shigella sonnei and Shigella flexneri
J. Med. Microbiol. 67, 376 (2018); https://doi.org/10.1099/jmm.0.000683
/content/journal/jmm/10.1099/jmm.0.000683
/content/journal/jmm/10.1099/jmm.0.000683
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