Denture-associated biofilm infection in three-dimensional oral mucosal tissue models Open Access

Abstract

analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The -related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs. The aim of this study was to develop and evaluate the use of tissue models to assess infections by biofilms on acrylic surfaces through tissue damage and virulence gene expression.

models were compared against commercially available tissue equivalents (keratinocyte-only, SkinEthic; full-thickness, MatTek Corporation). An keratinocyte-only tissue was produced using a cancer-derived cell line, TR146, and a full-thickness model incorporating primary fibroblasts and immortalised normal oral keratinocytes was also generated. The full-thickness tissues incorporated keratinocytes and fibroblasts, and have potential for future further development and analysis.

Following polymicrobial infection with biofilms on acrylic surfaces, both in-house developed models were shown to provide equivalent results to the SkinEthic and MatTek models in terms of tissue damage: a significant (<0.05) increase in LDH activity for mixed species biofilms compared to uninfected control, and no significant difference (>0.05) in the expression of most virulence genes when comparing tissue models of the same type.

Our results confirm the feasibility and suitability of using these alternative tissue models for such analyses.

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2018-03-01
2024-03-29
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