1887

Journal of Medical Microbiology logo

Volume 66, Issue 12

Short-term incubation of positive blood cultures in brain-heart infusion broth accelerates identification of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry Free

Abstract

Fast identification of bacteria directly from positive blood cultures (BCs) by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) can be achieved either using the MALDI Sepsityper kit (protein extraction method) or after a short-term pre-cultivation step on solid medium. We developed a new method that involves short-term enrichment of positive BCs in brain–heart infusion broth (BHI) prior to MALDI-TOF MS analysis.

Eighty-four BCs flagged as positive were included in this study; these were processed in parallel either directly using the MALDI Sepsityper kit or following a short-term culture either in BHI or on Columbia blood agar with 5 % sheep blood (CBA).

Bacterial species were successfully identified in 91.6, 89.2 and 65.4 % of cases after pre-cultivation for 4 h in BHI, on CBA, or by using the MALDI Sepsityper kit, respectively. Overall, the mean incubation time to correct identification was shorter when pre-cultures were performed in BHI; the mean time for Gram-negative rods was 78.2 min in BHI and 108.2 min on CBA (=0.045), and the mean time for Gram-positive cocci was 128.5 min in BHI and 169.6 min on CBA (=0.013).

Short-term enrichment of BCs in BHI accelerates identification of a number of bacterial species by MALDI-TOF MS. Further prospective studies are needed to validate our method and gauge its potential clinical impact on the management of bloodstream bacterial infections.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): blood cultures , broth incubation , fast identification , MALDI-TOF MS and short-term incubation
© 2017 The Authors
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/content/journal/jmm/10.1099/jmm.0.000643
2017-12-01
2024-04-16
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Short-term incubation of positive blood cultures in brain-heart infusion broth accelerates identification of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry
J. Med. Microbiol. 66, 1752 (2017); https://doi.org/10.1099/jmm.0.000643
/content/journal/jmm/10.1099/jmm.0.000643
/content/journal/jmm/10.1099/jmm.0.000643
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