1887

Abstract

Alternatives to antibiotic therapy for mastitis in ruminants are needed. We present an evaluation, in two trials, of the efficacy of an intramammary infusion of a live culture of for the treatment of subclinical and clinical mastitis in ewes.

In total, 67 animals were enrolled: 19 lactating ewes (study 1), including healthy (=6) and coagulase-negative staphylococci (CNS)-infected ewes (=13); and 48 lactating ewes (study 2) with either CNS mastitis (=32), or mastitis (=16), for a total of 123 mammary glands. Intramammary infusions were performed with either or PBS for 3 (study 1) or 7 (study 2) consecutive days. Antibiotic-treated and untreated control glands were included. Milk samples for microbiology, somatic cell analysis and milk production were collected before and after treatment.

rapidly activated the mammary glands' innate immune response and initiated an inflammatory response as evidenced by the recruitment of polymorphonuclear neutrophils and increased somatic cell counts. But while leading to a transient clearance of CNS in the gland, this response caused mild to moderate clinical cases of mastitis characterized by abnormal milk secretions and udder inflammation. Moreover, infections did not improve, and CNS infections tended to relapse.

Under our experimental conditions, the treatment led to a transient clearance of the pathogen in the gland, but also caused mild to moderate clinical cases of mastitis. We believe it is still early to implement bacterial formulations as alternatives in treating mastitis in ruminants and further experimentation is needed.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000641
2017-12-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/12/1798.html?itemId=/content/journal/jmm/10.1099/jmm.0.000641&mimeType=html&fmt=ahah

References

  1. Bergonier D, de Crémoux R, Rupp R, Lagriffoul G, Berthelot X. Mastitis of dairy small ruminants. Vet Res 2003; 34:689–716 [View Article][PubMed]
    [Google Scholar]
  2. Contreras A, Sierra D, Sánchez A, Corrales JC, Marco JC et al. Mastitis in small ruminants. Small Ruminant Research 2007; 68:145–153 [View Article]
    [Google Scholar]
  3. Gonzalo C, Ariznabarreta A, Carriedo JA, San Primitivo F. Mammary pathogens and their relationship to somatic cell count and milk yield losses in dairy ewes. J Dairy Sci 2002; 85:1460–1467 [View Article][PubMed]
    [Google Scholar]
  4. Onni T, Sanna G, Cubeddu GP, Marogna G, Lollai S et al. Identification of coagulase-negative staphylococci isolated from ovine milk samples by PCR-RFLP of 16S rRNA and gap genes. Vet Microbiol 2010; 144:347–352 [View Article][PubMed]
    [Google Scholar]
  5. Gelasakis AI, Mavrogianni VS, Petridis IG, Vasileiou NG, Fthenakis GC. Mastitis in sheep-The last 10 years and the future of research. Vet Microbiol 2015; 181:136–146 [View Article][PubMed]
    [Google Scholar]
  6. Knight CH, Fitzpatrick JL, Logue DN, Platt DJ. Efficacy of two non-antibiotic therapies, oxytocin and topical liniment, against bovine staphylococcal mastitis. Vet Rec 2000; 146:311–316 [View Article][PubMed]
    [Google Scholar]
  7. Werner C, Sobiraj A, Sundrum A. Efficacy of homeopathic and antibiotic treatment strategies in cases of mild and moderate bovine clinical mastitis. J Dairy Res 2010; 77:460–467 [View Article][PubMed]
    [Google Scholar]
  8. Cao LT, Wu JQ, Xie F, Hu SH, Mo Y. Efficacy of nisin in treatment of clinical mastitis in lactating dairy cows. J Dairy Sci 2007; 90:3980–3985 [View Article][PubMed]
    [Google Scholar]
  9. Wu J, Hu S, Cao L. Therapeutic effect of nisin Z on subclinical mastitis in lactating cows. Antimicrob Agents Chemother 2007; 51:3131–3135 [View Article][PubMed]
    [Google Scholar]
  10. Ryan MP, Flynn J, Hill C, Ross RP, Meaney WJ. The natural food grade inhibitor, lacticin 3147, reduced the incidence of mastitis after experimental challenge with Streptococcus dysgalactiae in nonlactating dairy cows. J Dairy Sci 1999; 82:2625–2631 [View Article][PubMed]
    [Google Scholar]
  11. Twomey DP, Wheelock AI, Flynn J, Meaney WJ, Hill C et al. Protection against Staphylococcus aureus mastitis in dairy cows using a bismuth-based teat seal containing the bacteriocin, lacticin 3147. J Dairy Sci 2000; 83:1981–1988 [View Article][PubMed]
    [Google Scholar]
  12. Klostermann K, Crispie F, Flynn J, Ross RP, Hill C et al. Intramammary infusion of a live culture of Lactococcus lactis for treatment of bovine mastitis: comparison with antibiotic treatment in field trials. J Dairy Res 2008; 75:365–373 [View Article][PubMed]
    [Google Scholar]
  13. Fragkou IA, Boscos CM, Fthenakis GC. Diagnosis of clinical or subclinical mastitis in ewes. Small Rumin Res 2014; 118:86–92 [View Article]
    [Google Scholar]
  14. Pinzón-Sánchez C, Ruegg PL. Risk factors associated with short-term post-treatment outcomes of clinical mastitis. J Dairy Sci 2011; 94:3397–3410 [View Article][PubMed]
    [Google Scholar]
  15. Holt JC, Krieg NR, Sneath PHA, Staley JT, Williams ST et al. Bergey's Manual of Determinative Bacteriology, 9th ed. Baltimore, MD: William & Wilkins; 1994
    [Google Scholar]
  16. Crispie F, Alonso-Gómez M, O'Loughlin C, Klostermann K, Flynn J et al. Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live lactococci on the mammary immune response. J Dairy Res 2008; 75:374–384 [View Article][PubMed]
    [Google Scholar]
  17. Beecher C, Daly M, Berry DP, Klostermann K, Flynn J et al. Administration of a live culture of Lactococcus lactis DPC 3147 into the bovine mammary gland stimulates the local host immune response, particularly IL-1beta and IL-8 gene expression. J Dairy Res 2009; 76:340–348 [View Article][PubMed]
    [Google Scholar]
  18. Enriquez-Valencia CE, Serna-Cock L, Campos-Gaona R. Intramammary infusion of Weissella confusa affects somatic cells counts and milk differential leukocyte count. Ciencia Rural 2015; 45:835–841 [Crossref]
    [Google Scholar]
  19. Frola ID, Pellegrino MS, Espeche MC, Giraudo JA, Nader-Macias ME et al. Effects of intramammary inoculation of Lactobacillus perolens CRL1724 in lactating cows' udders. J Dairy Res 2012; 79:84–92 [View Article][PubMed]
    [Google Scholar]
  20. Serna-Cock L, Enriquez CE, Gaona RC, Vasquez A. Cellular response of the bovine mammary gland after Weissella confusa infusion to control Streptococcus agalactiae . Revista Colombiana De Ciencias Pecuarias 2013; 26:280–287
    [Google Scholar]
  21. Armas F, Camperio C, Marianelli C. In Vitro assessment of the probiotic potential of Lactococcus lactis LMG 7930 against ruminant mastitis-causing pathogens. PLoS One 2017; 12:e0169543 [View Article][PubMed]
    [Google Scholar]
  22. Sears PM, Smith BS, English PB, Herer PS, Gonzalez RN. Shedding pattern of Staphylococcus aureus from bovine intramammary infections. J Dairy Sci 1990; 73:2785–2789 [View Article][PubMed]
    [Google Scholar]
  23. Newbould FHS, Neave FK. The recovery of small numbers of Staphylococcus aureus infused into the bovine teat cistern. J Dairy Res 1965; 32:157–162 [View Article]
    [Google Scholar]
  24. Hill AW, Shears AL, Hibbitt KG. The elimination of serum-resistant Escherichia coli from experimentally infected single mammary glands of healthy cows. Res Vet Sci 1978; 25:89–93[PubMed]
    [Google Scholar]
  25. Greene WA, Gano AM, Smith KL, Hogan JS, Todhunter DA. Comparison of probiotic and antibiotic intramammary therapy of cattle with elevated somatic cell counts. J Dairy Sci 1991; 74:2976–2981 [View Article][PubMed]
    [Google Scholar]
  26. Frola ID, Pellegrino MS, Magnano G, Giraudo JA, Espeche MC et al. Histological examination of non-lactating bovine udders inoculated with Lactobacillus perolens CRL 1724. J Dairy Res 2013; 80:28–35 [View Article][PubMed]
    [Google Scholar]
  27. Roumen MP, Sinke M, Gruys E. [Iatrogenic mastitis in sheep]. Tijdschr Diergeneeskd 1987; 112:513–518[PubMed]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.000641
Loading
/content/journal/jmm/10.1099/jmm.0.000641
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error