1887

Abstract

Purpose. Bartonella is an increasingly isolated emerging pathogen that can cause severe illness in humans, including cat scratch disease (CSD). The bacteria are difficult to grow and thus many detection methods have been developed, especially molecular. We previously developed a PCR method targeting ribC to identify Bartonella sp. A manufactured kit (RealCycler BART, Progenie Molecular) was commercialised shortly thereafter for the detection of Bartonella infection, including Bartonella henselae.

Methodology. We performed a comparison between this test and our in-house PCR assay on 73 lymphadenopathy samples sent to the laboratory for suspicion of CSD.

Results/Key findings. Among the 28 positive samples for Bartonella, 21 were identified by the two PCR assays, and seven by the commercial kit only.

Conclusion. The performance of this commercial kit suggests that it could be a suitable alternative to our in-house PCR assay, highlighting the importance of the molecular methods used to diagnose CSD.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000626
2017-10-25
2019-08-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/11/1596.html?itemId=/content/journal/jmm/10.1099/jmm.0.000626&mimeType=html&fmt=ahah

References

  1. Debré R, Job J-C. La maladie des griffes du chat (cat scratch fever). Acta Paediatr 1954; 43: 386– 389 [CrossRef]
    [Google Scholar]
  2. English CK, Wear DJ, Margileth AM, Lissner CR, Walsh GP. Cat-scratch disease. Isolation and culture of the bacterial agent. JAMA 1988; 259: 1347– 1352 [CrossRef] [PubMed]
    [Google Scholar]
  3. Chomel BB. Cat-scratch disease and bacillary angiomatosis. Rev Sci Tech 1996; 15: 1061– 1073 [CrossRef] [PubMed]
    [Google Scholar]
  4. Brenner DJ, O'Connor SP, Winkler HH, Steigerwalt AG. Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int J Syst Bacteriol 1993; 43: 777– 786 [CrossRef] [PubMed]
    [Google Scholar]
  5. Jacomo V, Kelly PJ, Raoult D. Natural history of Bartonella infections (an exception to Koch's postulate). Clin Diagn Lab Immunol 2002; 9: 8– 18 [PubMed]
    [Google Scholar]
  6. Rolain JM, Brouqui P, Koehler JE, Maguina C, Dolan MJ et al. Recommendations for treatment of human infections caused by Bartonella species. Antimicrob Agents Chemother 2004; 48: 1921– 1933 [CrossRef] [PubMed]
    [Google Scholar]
  7. Zeaiter Z, Fournier PE, Ogata H, Raoult D. Phylogenetic classification of Bartonella species by comparing groEL sequences. Int J Syst Evol Microbiol 2002; 52: 165– 171 [CrossRef] [PubMed]
    [Google Scholar]
  8. Paziewska A, Harris PD, Zwolińska L, Bajer A, Siński E. Recombination within and between species of the alpha proteobacterium Bartonella infecting rodents. Microb Ecol 2011; 61: 134– 145 [CrossRef] [PubMed]
    [Google Scholar]
  9. Johnson G, Ayers M, McClure SC, Richardson SE, Tellier R. Detection and identification of Bartonella species pathogenic for humans by PCR amplification targeting the riboflavin synthase gene (ribC). J Clin Microbiol 2003; 41: 1069– 1072 [CrossRef] [PubMed]
    [Google Scholar]
  10. Zeaiter Z, Fournier PE, Greub G, Raoult D. Diagnosis of Bartonella endocarditis by a real-time nested PCR assay using serum. J Clin Microbiol 2003; 41: 919– 925 [CrossRef] [PubMed]
    [Google Scholar]
  11. Leclainche L, Bourrillon A. Maladies des griffes du chat chez l'enfant immunocompétent. Archives de Pédiatrie 1996; 3: 378– 382 [CrossRef]
    [Google Scholar]
  12. Koehler JE, Sanchez MA, Garrido CS, Whitfeld MJ, Chen FM et al. Molecular epidemiology of Bartonella infections in patients with bacillary angiomatosis-peliosis. N Engl J Med 1997; 337: 1876– 1883 [CrossRef] [PubMed]
    [Google Scholar]
  13. Baty G, Lanotte P, Hocqueloux L, Prazuck T, Bret L et al. PCR rDNA 16S dans le diagnostic étiologique des endocardites infectieuses à hémocultures négatives. Med Mal Infect 2010; 40: 358– 362 [CrossRef]
    [Google Scholar]
  14. Parra E, Segura F, Tijero J, Pons I, Nogueras MM. Development of a real-time PCR for Bartonella spp. detection, a current emerging microorganism. Mol Cell Probes 2017; 32: 55– 59 [CrossRef] [PubMed]
    [Google Scholar]
  15. Bergmans AM, Groothedde JW, Schellekens JF, van Embden JD, Ossewaarde JM et al. Etiology of cat scratch disease: comparison of polymerase chain reaction detection of Bartonella (formerly Rochalimaea) and Afipia felis DNA with serology and skin tests. J Infect Dis 1995; 171: 916– 923 [CrossRef] [PubMed]
    [Google Scholar]
  16. Dupon M, Savin de Larclause AM, Brouqui P, Drancourt M, Raoult D et al. Evaluation of serological response to Bartonella henselae, Bartonella quintana and Afipia felis antigens in 64 patients with suspected cat-scratch disease. Scand J Infect Dis 1996; 28: 361– 366 [CrossRef] [PubMed]
    [Google Scholar]
  17. Houpikian P, Raoult D. Molecular phylogeny of the genus Bartonella: what is the current knowledge?. FEMS Microbiol Lett 2001; 200: 1– 7 [CrossRef] [PubMed]
    [Google Scholar]
  18. Jacobs RF, Schutze GE. Bartonella henselae as a cause of prolonged fever and fever of unknown origin in children. Clin Infect Dis 1998; 26: 80– 84 [CrossRef] [PubMed]
    [Google Scholar]
  19. Melenotte C, Edouard S, Lepidi H, Raoult D. Diagnostic des adénites infectieuses. Rev Médecine Interne 2015; 36: 668– 676 [Crossref]
    [Google Scholar]
  20. Lamps LW, Scott MA. Cat-scratch disease: historic, clinical, and pathologic perspectives. Am J Clin Pathol 2004; 121: S71– S80 [PubMed]
    [Google Scholar]
  21. Fournier PE, Mainardi JL, Raoult D. Value of microimmunofluorescence for diagnosis and follow-up of Bartonella endocarditis. Clin Diagn Lab Immunol 2002; 9: 795– 801 [CrossRef] [PubMed]
    [Google Scholar]
  22. Fowler RS, Bailey JD. Cat scratch disease in childhood. Can Med Assoc J 1961; 84: 1365– 1368 [PubMed]
    [Google Scholar]
  23. Gutiérrez R, Vayssier-Taussat M, Buffet JP, Harrus S. Guidelines for the isolation, molecular detection, and characterization of Bartonella species. Vector Borne Zoonotic Dis 2017; 17: 42– 50 [CrossRef] [PubMed]
    [Google Scholar]
  24. Regnery RL, Anderson BE, Clarridge JE, Rodriguez-Barradas MC, Jones DC et al. Characterization of a novel Rochalimaea species, R. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient. J Clin Microbiol 1992; 30: 265– 274 [PubMed]
    [Google Scholar]
  25. Michael F, Minnick KDB. Identification of Bartonella using PCR; genus- and species-specific primer sets. J Microbiol Methods 1997; 31: 51– 57 [Crossref]
    [Google Scholar]
  26. Norman AF, Regnery R, Jameson P, Greene C, Krause DC. Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J Clin Microbiol 1995; 33: 1797– 1803 [PubMed]
    [Google Scholar]
  27. Jensen WA, Fall MZ, Rooney J, Kordick DL, Breitschwerdt EB. Rapid identification and differentiation of Bartonella species using a single-step PCR assay. J Clin Microbiol 2000; 38: 1717– 1722 [PubMed]
    [Google Scholar]
  28. Houpikian P, Raoult D. 16S/23S rRNA intergenic spacer regions for phylogenetic analysis, identification, and subtyping of Bartonella species. J Clin Microbiol 2001; 39: 2768– 2778 [CrossRef] [PubMed]
    [Google Scholar]
  29. La Scola B, Raoult D. Culture of Bartonella quintana and Bartonella henselae from human samples: a 5-year experience (1993 to 1998). J Clin Microbiol 1999; 37: 1899– 1905 [PubMed]
    [Google Scholar]
  30. Bereswill S, Hinkelmann S, Kist M, Sander A. Molecular analysis of riboflavin synthesis genes in Bartonella henselae and use of the ribC gene for differentiation of Bartonella species by PCR. J Clin Microbiol 1999; 37: 3159– 3166 [PubMed]
    [Google Scholar]
  31. Roux V, Raoult D. Inter- and intraspecies identification of Bartonella (Rochalimaea) species. J Clin Microbiol 1995; 33: 1573– 1579 [PubMed]
    [Google Scholar]
  32. Marston EL, Sumner JW, Regnery RL. Evaluation of intraspecies genetic variation within the 60 kDa heat-shock protein gene (groEL) of Bartonella species. Int J Syst Bacteriol 1999; 49: 1015– 1023 [CrossRef] [PubMed]
    [Google Scholar]
  33. Renesto P, Gouvernet J, Drancourt M, Roux V, Raoult D. Use of rpoB gene analysis for detection and identification of Bartonella species. J Clin Microbiol 2001; 39: 430– 437 [CrossRef] [PubMed]
    [Google Scholar]
  34. Avidor B, Kletter Y, Abulafia S, Golan Y, Ephros M et al. Molecular diagnosis of cat scratch disease: a two-step approach. J Clin Microbiol 1997; 35: 1924– 1930 [PubMed]
    [Google Scholar]
  35. Li W, Raoult D, Fournier PE. Genetic diversity of Bartonella henselae in human infection detected with multispacer typing. Emerg Infect Dis 2007; 13: 1178– 1183 [CrossRef] [PubMed]
    [Google Scholar]
  36. Zeaiter Z, Fournier PE, Raoult D. Genomic variation of Bartonella henselae strains detected in lymph nodes of patients with cat scratch disease. J Clin Microbiol 2002; 40: 1023– 1030 [CrossRef] [PubMed]
    [Google Scholar]
  37. Ridder GJ, Boedeker CC, Technau-Ihling K, Grunow R, Sander A. Role of cat-scratch disease in lymphadenopathy in the head and neck. Clin Infect Dis 2002; 35: 643– 649 [CrossRef] [PubMed]
    [Google Scholar]
  38. Bergmans AM, Peeters MF, Schellekens JF, Vos MC, Sabbe LJ et al. Pitfalls and fallacies of cat scratch disease serology: evaluation of Bartonella henselae-based indirect fluorescence assay and enzyme-linked immunoassay. J Clin Microbiol 1997; 35: 1931– 1937 [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000626
Loading
/content/journal/jmm/10.1099/jmm.0.000626
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error