1887

Abstract

Raw milk has recently been reported as a source of extended-spectrum beta-lactamase (ESBL) and carbapenemase genes. We thus investigated the prevalence of ESBL- and carbapenemase-producing in raw milk in Lebanon in order to assess the risk of transfer of these bacteria to humans. A high prevalence (30.2 %) of CTX-M-15-producing was detected in raw bovine milk. Three main clones were identified by PFGE and MLST typing. Southern blot experiments revealed that one of these clones carried the gene chromosomally. Moreover, one OXA-48-producing ST530 and seven CTX-M-15-producing sharing the same ST were also detected. These findings highlight the spread of dominant CTX-M-15-producing clones and OXA-48-producing isolates in the food chain. Milk, which is mostly consumed raw in Lebanon, may be a source of human exposure to ESBLs and carbapenemases.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000620
2017-11-01
2024-11-11
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/11/1688.html?itemId=/content/journal/jmm/10.1099/jmm.0.000620&mimeType=html&fmt=ahah

References

  1. Zurfluh K, Nüesch-Inderbinen MT, Poirel L, Nordmann P, Hächler H et al. Emergence of Escherichia coli producing OXA-48 β-lactamase in the community in Switzerland. Antimicrob Resist Infect Control 2015; 4:9 [View Article][PubMed]
    [Google Scholar]
  2. Beyrouthy R, Robin F, Dabboussi F, Mallat H, Hamzé M et al. Carbapenemase and virulence factors of Enterobacteriaceae in North Lebanon between 2008 and 2012: evolution via endemic spread of OXA-48. J Antimicrob Chemother 2014; 69:2699–2705 [View Article][PubMed]
    [Google Scholar]
  3. Potron A, Poirel L, Rondinaud E, Nordmann P. Intercontinental spread of OXA-48 beta-lactamase-producing Enterobacteriaceae over a 11-year period, 2001 to 2011. Euro Surveill 2013; 18:20549 [View Article][PubMed]
    [Google Scholar]
  4. Yaici L, Haenni M, Métayer V, Saras E, Mesbah Zekar F et al. Spread of ESBL/AmpC-producing Escherichia coli and Klebsiella pneumoniae in the community through ready-to-eat sandwiches in Algeria. Int J Food Microbiol 2017; 245:66–72 [View Article][PubMed]
    [Google Scholar]
  5. Zurfluh K, Nüesch-Inderbinen M, Morach M, Zihler Berner A, Hächler H et al. Extended-spectrum-β-lactamase-producing Enterobacteriaceae isolated from vegetables imported from the Dominican Republic, India, Thailand, and Vietnam. Appl Environ Microbiol 2015; 81:3115–3120 [View Article][PubMed]
    [Google Scholar]
  6. Reuland EA, Al Naiemi N, Raadsen SA, Savelkoul PH, Kluytmans JA et al. Prevalence of ESBL-producing Enterobacteriaceae in raw vegetables. Eur J Clin Microbiol Infect Dis 2014; 33:1843–1846 [View Article][PubMed]
    [Google Scholar]
  7. Odenthal S, Akineden Ö, Usleber E. Extended-spectrum β-lactamase producing Enterobacteriaceae in bulk tank milk from German dairy farms. Int J Food Microbiol 2016; 238:72–78 [View Article][PubMed]
    [Google Scholar]
  8. Sudarwanto M, Akineden Ö, Odenthal S, Gross M, Usleber E. Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae in bulk tank milk from dairy farms in Indonesia. Foodborne Pathog Dis 2015; 12:585–590 [View Article][PubMed]
    [Google Scholar]
  9. Bhoomika, Shakya S, Patyal A, Gade NE. Occurrence and characteristics of extended-spectrum β-lactamases producing Escherichia coli in foods of animal origin and human clinical samples in Chhattisgarh, India. Vet World 2016; 9:996–1000 [View Article][PubMed]
    [Google Scholar]
  10. Skočková A, Bogdanovičoá K, Koláčková I, Karpíšková R. Antimicrobial-resistant and extended-spectrum β-lactamase-producing Escherichia coli in raw cow's milk. J Food Prot 2015; 78:72–77 [View Article][PubMed]
    [Google Scholar]
  11. Koovapra S, Bandyopadhyay S, Das G, Bhattacharyya D, Banerjee J et al. Molecular signature of extended spectrum β-lactamase producing Klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern India. Infection, Genetics and Evolution 2016; 44:395–402 [View Article]
    [Google Scholar]
  12. Yaici L, Haenni M, Saras E, Boudehouche W, Touati A et al. bla NDM-5-carrying IncX3 plasmid in Escherichia coli ST1284 isolated from raw milk collected in a dairy farm in Algeria. J Antimicrob Chemother 2016; 71:2671–2672 [View Article][PubMed]
    [Google Scholar]
  13. Diab M, Hamze M, Madec JY, Haenni M. High prevalence of non-ST131 CTX-M-15-producing Escherichia coli in healthy cattle in Lebanon. Microb Drug Resist 2017; 23:261–266 [Crossref]
    [Google Scholar]
  14. Al Bayssari C, Olaitan AO, Dabboussi F, Hamze M, Rolain JM. Emergence of OXA-48-producing Escherichia coli clone ST38 in fowl. Antimicrob Agents Chemother 2015; 59:745–746 [View Article][PubMed]
    [Google Scholar]
  15. Munoz MA, Bennett GJ, Ahlström C, Griffiths HM, Schukken YH et al. Cleanliness scores as indicator of Klebsiella exposure in dairy cows. J Dairy Sci 2008; 91:3908–3916 [View Article][PubMed]
    [Google Scholar]
  16. Wang G, Huang T, Surendraiah PK, Wang K, Komal R et al. CTX-M β-lactamase-producing Klebsiella pneumoniae in suburban New York City, New York, USA. Emerg Infect Dis 2013; 19:1803–1810 [View Article][PubMed]
    [Google Scholar]
  17. Löhr IH, Hülter N, Bernhoff E, Johnsen PJ, Sundsfjord A et al. Persistence of a pKPN3-like CTX-M-15-encoding IncFIIK plasmid in a Klebsiella pneumonia ST17 host during two years of intestinal colonization. PLoS One 2015; 10:e0116516 [View Article][PubMed]
    [Google Scholar]
  18. Zhang J, Zhou K, Zheng B, Zhao L, Shen P et al. High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China. Front Microbiol 2016; 7:1830 [View Article][PubMed]
    [Google Scholar]
  19. Rettedal S, Löhr IH, Natås O, Giske CG, Sundsfjord A et al. First outbreak of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in a Norwegian neonatal intensive care unit; associated with contaminated breast milk and resolved by strict cohorting. APMIS 2012; 120:612–621 [View Article][PubMed]
    [Google Scholar]
  20. Chamoun K, Farah M, Araj G, Daoud Z, Moghnieh R et al. Surveillance of antimicrobial resistance in Lebanese hospitals: retrospective nationwide compiled data. Int J Infect Dis 2016; 46:64–70 [View Article][PubMed]
    [Google Scholar]
  21. Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 2011; 70:119–123 [View Article]
    [Google Scholar]
  22. Eckert C, Gautier V, Saladin-Allard M, Hidri N, Verdet C et al. Dissemination of CTX-M-type beta-lactamases among clinical isolates of Enterobacteriaceae in Paris, France. Antimicrob Agents Chemother 2004; 48:1249–1255 [View Article][PubMed]
    [Google Scholar]
  23. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL et al. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 2005; 63:219–228 [View Article][PubMed]
    [Google Scholar]
  24. Mathers AJ, Peirano G, Pitout JD. The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae . Clin Microbiol Rev 2015; 28:565–591 [View Article][PubMed]
    [Google Scholar]
  25. Yang QE, Sun J, Li L, Deng H, Liu BT et al. IncF plasmid diversity in multi-drug resistant Escherichia coli strains from animals in China. Front Microbiol 2015; 6:964 [View Article][PubMed]
    [Google Scholar]
  26. Rodríguez I, Thomas K, Van Essen A, Schink AK, Day M et al. Chromosomal location of bla CTX-M genes in clinical isolates of Escherichia coli from Germany, The Netherlands and the UK. Int J Antimicrob Agents 2014; 43:553–557 [View Article][PubMed]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.000620
Loading
/content/journal/jmm/10.1099/jmm.0.000620
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error