1887

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Volume 66, Issue 10

Characterization of vaginal lactobacilli from HIV-negative and HIV-positive Indian women and their association with genital HIV-1 shedding Free

Abstract

One of the crucial determinants for successful administration of lactobacilli to the vaginal niche is the use of appropriate species. In this cross-sectional study 54 human immunodeficiency virus (HIV)-negative and 76 HIV-positive antiretroviral treatment-naïve women were evaluated for culturable vaginal lactobacilli and their association with genital HIV-1 shedding. species were identified by 16S rDNA sequencing while cervical and plasma HIV-1 viral load was determined by Abbott real-time PCR. Lactobacilli were isolated in 77.8 % HIV-negative and 73.7 % HIV-positive women. The mean log plasma and cervical HIV-1 viral loads (RNA copies ml) were 3.73±1.02 and 2.85±0.32 respectively. We observed that presence of , or species was associated with undetectable cervical HIV-1 (=0.046) and reduced genital HIV-1 shedding (=0.048) compared to other species. Our findings endorse using -based strategies to aid the prevention of HIV-1 transmission among Indian women, however confirmation by future prospective studies is indeed warranted.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rDNA sequencing , HIV-1 shedding and vaginal Lactobacillus species
© 2017 The Authors
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2017-10-01
2024-04-19
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Characterization of vaginal lactobacilli from HIV-negative and HIV-positive Indian women and their association with genital HIV-1 shedding
J. Med. Microbiol. 66, 1471 (2017); https://doi.org/10.1099/jmm.0.000599
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